Effects of the Initial Rice Bran Concentration on the Production of Lactobacillus casei as Digestive Bio-regulator


  • Siti Marsilawati Mohamed Esivan Department of Bioprocess Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia
  • Roslina Rashid Department of Bioprocess Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia
  • Nor Azrini Nadiha Azmi Department of Bioprocess Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia
  • Nor Athirah Zaharudin Department of Bioprocess Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia
  • Norasikin Othman Centre of Lipid Engineering & Applied Research (CLEAR), Ibnu Sina Institute for Scientific and Industrial Research, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia




Lactobacillus casei, viability, probiotics, rice bran, agro waste


Digestive bio-regulator or probiotic is defined as live bacteria with clinically documented health effects in humans and animals. Nowadays, there is increasing interest in probiotics from all over the world. However, the cost of producing probiotics products is still high. To reduce the cost, there is a need to study the usage of agro waste as an inexpensive substrate. The viability of the cell was counted in colony unit per mL (CFU/mL) and the growth was measured using dry weight measurement (g/mL). The sugar concentration was measured using glucose analyzer. At initial substrate concentration of 20% (w/v) and at incubation time of 10hr, the viability cell was 3 x 108 CFU/mL and cell dry weight was 0.0076 g/mL. From the results of this study, it is found that when the initial substrate concentration increased, the viability and growth of Lactobacillus casei increased.


Fuller, R. 1995. Probiotics: Their Development and Use. In R. Fuller, P.J. Heidt, V. Rusch, D.V.D. Waaij (Eds.). Old Herborn University Seminar Monograph 8. Probiotics: Prospects of use in Opportunistic Infections Herborn-Dill, Germany: The Institute for Microbiology and Biochemistry. 1–8.

Fooks, L. J., Fuller, R., Gibson, G. R. 1999. Prebiotics, Probiotics and Human Gut Microbiology. International Dairy Journal. 9: 53–61.

Patterson, J. A. 2012. The Commensal Microbiota. In T. R. Callaway, S. C. Ricke (Eds.). Direct Fed Microbials and Prebiotics for Animals 1st Edition. London: Springer Science+Business Media. 3–11.

Fuller, R. 1989. Probiotics in Man and Animal. J. Appl. Bacteriol. 66: 365–378.

Malik, R. and Bandla, S. 2010. Effect of Source and Dose of Probiotics and Exogenous Fibrolytic Enzymes (EFE) on Intake, Feed Efficiency, and Growth of Male Buffalo (Bubalus bubalis) calves. Trop. Anim. Health Prod. 42: 1263–1269.

Seo, J. K., Kim, S. W., Kim, M. H., Upadhaya, S. D., Kam, D. K., Ha, J. K. 2010. Direct-fed Microbial for Ruminant Animals. Asian-Aust. J. Anim. Sci. 23(12): 1657–1667.

Cheng, G., Hao, H., Xie, S., Wang, X., Dai, M., Huang, L., Yuan, Z. 2014. Antibiotic Alternatives: The Substitution of Antibiotics in Animal Husbandry? Frontiers in Microbiology. 5: 1–15. doi:10.3389/fmicb.2014.00217.

Stanton, T. B. 2013. A Call for Antibiotics Alternatives Research. Trends in Microbiology. 21: 111–113. doi:10.1016/j.tim.2012.11.002.

Allen, H. K., Levine, U. Y., Looft, T., Bandrick, M., and Casey, T. A. 2013. Treatment, Promotion, Commotion: Antibiotic Alternatives in Food-Producing Animals. Trends in Microbiology. 21(3): 114–119.

Castanon, J. J. 2007. History of the Use of Antibiotic as Growth Promoters in European Poultry Feeds. Poult. Sci. 86: 2466–2471. doi:10.3382/ps.2007-00249.

Adams, M. C., Luo, J., Rayward, D., King, S., Gibson, R., Moghaddam, G. H. 2008. Selection of a Novel Direct-Fed Microbial to Enhance Weight Gain in Intensively Reared Calves. Animal Feed Science and Technology. 145: 41–52.

Nocek, J. E. and W. P. Kautz. 2006. Direct-fed Microbial Supplementation on Ruminal Digestion, Health, and Performance of Pre- and Postpartum Dairy Cattle. J. Dairy Sci. 89: 260–266.

Weiss, W. P., D. J. Wyatt and T. R. McKelvey. 2008. Effect of Feeding Propionibacteria on Milk Production by Early Lactation Dairy Cows. J. Dairy Sci. 91: 646–652.

Bernaedeau, M. and Vernoux, J.-P. 2013. Overview of Differences between Microbial Feed Additives and Probitics for Food Regarding Regulation, Growth Promotion Effects, and Health Properties and Consequences for Extrapolation of Farm Animals to Humans. Clinical Microbiology and Infection. 19: 321–330.

Frizzo, L. S., L. P. Sotto, M. V. Zbrun, E. Bertozzi, G. Sequeira, R. R. Armesto and M. R. Rosmini. 2010. Lactic Acid Bacteria to Improve Growth Performance in Young Calves Fed Milk Replacer and Spray-Dried Whey Powder. Anim. Feed Sci. Technol. 157: 159–167.

Karunasena, E., Kurkure, P. C., Lackey, R. D., McMahon, K. W., Kiernan, E. P., Graham, S., Alabady, M. S., Campos, D. L., Tatum, O. L., Brashears, M. M. 2013. Effects of the Probiotic Lactobacillus animalis in Murine Mycobacterium avium subspecies paratuberculosis Infection. BMC Microbiology. 13: 8.


EFSA. 2007. European Food Safety Authority. Introduction of a Qualified Presumption Of Safety (QPS) Approach for Assessment of Selected Microorganisms Referred to EFSA Opinion of the Scientific Committee (Question No EFSA-Q-2005-293) Adopted on 19 November 2007. EFSA Journal. 587: 1–16.

Millette, M., Luquet, F. M., and Lacroix, M. 2007. In Vitro Growth of Selected Pathogens by Lactobacillus acidophilus- and Lactobacillus casei- Fermented Milk. Letters in Applied Microbiology. 44: 314–319.

Todhanakasem T, Nikapong P. 2012. Use of Rice Hull Hydrolyzate in the Cultivation of Lactobacillus acidophilus. KKU Research Journal. 17: 778–786.

Anvari, M., Khayati, G., Rostami. 2014. Optimisation of Medium Composition for Probiotic Biomass Production using Response Surface Methodology. Journal of Dairy Research. 81: 59–64.

Tomás, M. S. J., Bru, E., Wiese, B., Nader-Macías, M. E. F. 2010. Optimization of Low-Cost Culture Media for the Production of Biomass and Bacteriocin by Urogenital Lactobacillus salivarius Strain. Probiotics & Antimicro. Prot. 2: 2–11.

Yoon, K. Y., Woodams, E. E., Hang, Y. D. 2006. Production of Probiotic Cabbage Juice by Lactic Acid Bacteria. Bioresource Technology. 97: 1427–1430.

Elmarzugi, N., Enshasy, H. E., Abd Malek, R., Othman, Z., Sarmidi, M. R., Abdel Aziz, R. 2010. Optimization of Cell Mass Production of the Probiotic Strain Lactococcus lactis in a Batch and Fed-batch Culture in Pilot Scale Levels. In A. Mèndez-Vilas (Ed.). Current Research, Technology and Education Topics in Applied Microbiology and Microbial Technology Vol. 2. Spain: Formatex Research Centre, Badajoz. 873–879.

Aguirre-Ezkauriatza, E. J., Aguilar-Yanez, J. M., Ramirez-Medrano, A., Alvarez, M. M. 2010. Production of Probiotic Biomass (Lactobacillus casei) in Goat Milk Whey: Comparison of Batch, Continuous and Fed-Batch Cultures. Bioresource Technology. 101: 2837–2844.

Gao, M-T., Kaneko, M., Hirata, M., Toorisaka, E., and Hano, T. 2008. Utilization of Rice Bran as Nutrient Source for Fermentative Lactic Acid Production. Bioresource Technology. 99: 3659–3664.

Rosniyana, A., Hashifah, M. A., and Sharifah Norin, S. A. 2009. Nutritional Content and Storage Stability of Stabilized Rice Bran–MR 220. J. Trop. Agric. and Fd. Sc. 37(2): 163–170.

Faria, S. A. S. C., Bassinello, P. Z., Penteado, M. V. C. 2012. Nutritional Composition of Rice Bran Submitted to Different Stabilization Procedures. Brazillian Journal of Pharmaceutical Sciences. 48(4): 651–657.

Abdul-Hamid, A., Raja Sulaiman, R. R., Osman, A., Saari, N. 2007. Preliminary Study of the Chemical Composition of Rice Milling Fractions Stabilized by Microwave Heating. Journal of Food Composition and Analysis. 20: 627–637.

Elok Zubaidah, Nurcholis, M., Wulan, S. N., and Kusuma, A. 2012. Comparative Study on Synbiotic Effect of Fermented Rice Bran by Probiotic Lactic Acid Bacteria Lactobacillus casei and Newly Isolated Lactobacillus plantarum B2 in Winstar rats. APCBEE Procedia. 2: 170-177. ICBFS 2012: April 7-8, 2012, Bangkok, Thailand.

Tanaka, T., Hoshina, M., Tanabe, S., Sakai, K., Ohtsuba, S., Taniguchi, M. 2006. Production of D-lactic acid from Defatted Rice Bran by Simultaneous Saccharification and Fermentation. Bioresource Technology. 97: 211–217.

Watanabe, M., Makino, M., Kaku, N., Koyama, M., Nakamura, K., Sasano, K. 2013. Fermentative L-(+)-lactic Acid Production From Non-sterilized Rice Washing Drainage Containing Rice Bran by a Newly Isolated Lactic Acid Bacteria Without Any Additions of Nutrients. Journal of Bioscience and Bioengineering. 115(4): 449–452.

Shah, N. P. 2000. Probiotic Bacteria: Selective Enumeration and Survival in Dairy Foods. Journal of Dairy Science. 83: 894–907.




How to Cite

Mohamed Esivan, S. M., Rashid, R., Azmi, N. A. N., Zaharudin, N. A., & Othman, N. (2015). Effects of the Initial Rice Bran Concentration on the Production of Lactobacillus casei as Digestive Bio-regulator. Jurnal Teknologi, 74(7). https://doi.org/10.11113/jt.v74.4691



Science and Engineering