IDENTIFICATION OF TROPOMYOSIN AS THE MAJOR ALLERGEN OF BLACK TIGER PRAWN (PENAEUS MONODON) BY AN ALLERGENOMIC APPROACH
DOI:
https://doi.org/10.11113/jt.v77.6750Keywords:
Allergy, black tiger prawn, Penaeus monodon, proteomicsAbstract
Shellfish has been recognized as one of the leading causes of food allergy in both adults and children in Asia Pacific region. In Malaysia, black tiger prawn (Penaeus monodon) is among the most widely consumed species. Our previous studies have successfully identified several major allergens including a thermostable protein of 36 kDa. Thus the aim of this study was to identify the 36 kDa major allergen by an allergenomic approach. Protein extracts of raw prown were prepared and resolved by 2-dimensional electrophoresis (2-DE). Immunoblotting was then performed using sera from patients with prawn allergy. Selected spot from 2-DE was then excised, digested and analyzed by mass spectrometry. The 2-DE profile of the extract revealed approximately 100 protein spots between pH of ~4 to 10 and the size range between (<10 to 250 kDa). The 2-DE immunoblotting has detected numerous IgE-binding spots at 36 kDa. Mass spectrometry analysis of the major IgE-binding spot (spot 1a) has identified the 36 kDa spot as tropomyosin. Our findings indicated that tropomyosin play a major role in allergic reaction to black tiger prawn among local patients with prawn allergy, and should be included in diagnostics and therapeutic strategies of this allergy.
References
Lee, A. J., Gerez, I., Shek, L. P. & Lee, B. W. Shellfish allergy-an Asia-Pacific perspective. Asian Pacific Journal of Allergy and Immunology. 30(2012): 3-10.
Lopata, A. L. & Kamath, S. 2012. Shellfish Allergy Diagnosis- Gaps and Needs. Current Allergy and Clinical Immunology. 25(2): 60-66.
Lehrer, S.B., Ayuso, R. & Reese, G. Seafood Allergy and Allergens: A Review. Journal of Marine Biotechnology. 5(2003): 339-348.
Lopata, L. A. & Lehrer, S. B. 2009. New Insights Into Seafood Allergy. Current Opinion on Allergy and Clinical Immunology. 9: 270-277.
Shahnaz, M., Gendeh, B. S. & Nasuruddin, A. Skin Test Reactivity to Inhalant and Food Allergens in Patients with Allergic Rhinitis. International Medical Research Journal. 5(2001): 69-73.
Yadzir, Z. H. M., Misnan, R., Abdullah, N., Bakhtiar, F., Arip, M. & Murad, S. Identification of the Major Allergen of Macrobrachium Rosenbergii (Giant Freshwater Prawn). Asian Pacific Journal of Tropical Biomedicine. 2(2012): 50-54.
Reese, G., Ayuso, R. & Lehrer, S. B. 1999. Tropomyosin: An Invertebrate Pan-Allergen. International Archives of Allergy and Immunology. 119: 247-58.
Yu, C. J., Lin, Y. F., Chiang, B. L. & Chow, L. P. 2003. Proteomics and Immunological Analysis of a Novel Shrimp Allergen, Pen M 2. Journal of Immunology. 170: 445-453.
Garcia-Orozco, K. D., Aispuro-Hernandez, E., Yepiz-Plascencia, G., Calderon-de-la-Barca, & Sotelo-Mundo, R. R. 2007. Molecular Characterization of Arginine Kinase, An Allergen from Shrimp Litopenaeus vannamei. International Archives of Allergy and Immunology. 144: 23-28.
Ayuso, R.,Grishina, G., Ibanez, M. D., Blanco, C., Carrillo, T., Bencharitiwong, R., Sanchez, S., Wegrzyn, A.N. & Sampson, H. A. 2009. Sarcoplasmic Calcium-Binding Protein is an EF-Hand–Type Protein Identified as a New Shrimp Allergen. Journal of Allergy and Clinical Immunology. 124(1): 114-120.
Ayuso, R., Grishina, G., Bardina L., Carillo, T., Blanco, C., Ibanez, M. D., Sampson, H. A. & Beyer, K. 2008. Myosin Light Chain is a Novel Shrimp Allergen, Lit v 3. Journal of Allergy and Clinical Immunology. 122: 795-802.
Food and Agriculture Organization (FAO). Fishery Statistical Collections: Global Production Year 2012. Accessed April 10, 2014. http://www.fao.org/fishery/statistics/global-production/en.
Syuhaidah, S., Rosmilah, M., Zailatul, H. M. Y., Jamaludin, M., Noormalin, A., Faizal, B. & Shahnaz, M. 2011. Identification of Major and Minor Allergens of Black Tiger Prawn (Penaeus monodon) and King Prawn (Penaeus latisulcatus). Malaysian Journal of Medical Science. 18(3): 27-32.
Kukreja, N., Singh, B. P., Arora, N., Gaur, S. N. & Sridhara, S. (2008). Identification of Epicoccum purpurascens Allergen By Two-Dimensional Immunoblotting and Mass Spectrometry. Immunobiology. 213: 67-73.
Gill, B. V., Rice, T. R., Cartier, A., Gautrin, D., Neis, B., Horth-Susin, L., Jong, M., Swanson, M. & Lehrer, S. B. 2009. Identification of Crab Proteins that Elicit Ige Reactivity in Snow Crab-Processing Workers. Journal of Allergy and Clinical Immunology. 124: 1055-1061.
Abramovitch, J. B., Kamath, S., Varese, N., Zubrinich, C., Lopata, A. L., O'Hehir R. E., Rolland, J. M. 2013. IgE Reactivity of Blue Swimmer Crab (Portunus pelagicus) Tropomyosin, Por p 1, and Other Allergens; Cross-Reactivity with Black Tiger Prawn and Effects of Heating. PLoS One. 8(6): e67487.
Srinroch, C., Srisomsap, C., Chokchaichamnankit, D., Punyarit, P. & Phiriyangkul, P. 2015. Identification of Novel Allergen in Edible Insect, Gryllus Bimaculatus and Its Cross-Reactivity with Macrobrachium spp. allergens. Food Chemistry. 184: 160-166.
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