EFFECTS OF ETHANOL EXTRACTS OF GYNURA PROCUMBENS ON IN-VIVO PHAGOCYTOSIS OF WISTAR ALBINO RATS
DOI:
https://doi.org/10.11113/jt.v78.9090Keywords:
Gynura procumbens, wistar albino rat, immunomodulatory, phagocytic activity, haematological analysis, biochemical analysisAbstract
Gynura procumbens is a traditional medicinal plant that is commonly used in Southeast Asia for treatment of various ailments. Nonetheless, there is very limited information on the effect of Gynura procumbens on the immune system. This preliminary study evaluates Gynura procumbens immunomodulatory properties of ethanol extracts at four dose levels ranging from 100 to 400 mg/kg body weight by focusing on the effects on phagocytic activity of macrophages on healthy female Wistar albino rat models. Assessments on phagocytosis enhancement were evaluated using carbon clearance test together with the examination on the mortality, behavioral changes, blood cells count, and serum biochemistry. The result from carbon clearance test showed significant increase of phagocytic index (P<0.05) on Gynura procumbens extract treated group of all doses. Furthermore, this study also demonstrates that there were no significant changes in the general condition, haematological counts, and biochemistry values from the Gynura procumbens treated groups in comparison to the control group. These findings demonstrate the potential of Gynura procumbens as an immunomodulatory agent.
References
Shruti Shukla, Vivek K. Bajpai, Myunghee Kim. 2012. Plants as Potential Sources of Natural Immunomodulators. Reviews in Environmental Science and Bio/Technology. 1.
L. M. Perry. 1980. Attributed Properties and Uses. Medicinal Plants of East and Southeast Asia. London, UK: MIT Press.
Dwijayanti, Dinia Rizqi and Muhaimin Rifa. 2014. Immunomodulator Testing on Ethanol Extract of Gynura procumbens Leaves to Mus Musculus Adaptive Immune System: In Vitro Study. J. Exp. Life Sci. 4(1): 10-14.
Khalid Algariri, Item Justin Atangwho, Kuong Yow Meng, Mohd Zaini Asmawi, Amirin Sadikun and Vikneswaran Murugaiyah. 2014. Antihyperglycaemia. Tropical Life Sciences Research. 25(1): 75-93.
Hew, C. S. and L. H. Gam. 2014. The Identification of High Abundant Proteins in the Leaves of Gynura Procumbens. Biotechnology & Biotechnological Equipment. 24: 2132-36.
Zahra, A. A. et al. 2011. Acute Toxicity Study and Wound Healing Potential of Gynura procumbens Leaf Extract in Rats. Journal of Medicinal Plants Research. 5(12): 2551-58.
Mahmood, A. A., Abdalbasit A. Mariod, Fouad Al-bayaty, and Siddig Ibrahim Abdel-wahab. 2010. Anti-Ulcerogenic Activity of Gynura procumbens Leaf Extract against Experimentally-Induced Gastric Lesions in Rats. Journal of Medicinal Plants Research. 4(8): 685-91.
Rosidah et al. 2009. Toxicology Evaluation of Standardized Methanol Extract of Gynura Procumbens. Journal of ethnopharmacology. 123(2): 244-49.
Akowuah, G. A., Sadikun, A., & Mariam, A. 2002. Flavonoid Identification and Hypoglycaemic Studies of the Butanol Fraction from Gynura Procumbens. Pharmaceutical Biology. 40(6): 405-410.
Akinmoladun, Afolabi C., Ibukun, E. O., Afor, Emmanuel, Obuotor, E. M., & Farombi, E. O. 2007. Phytochemical Constituent and Antioxidant Activity of Extract from the Leaves of Ocimum Gratissimum. Sci. Res. Essay. 2(5): 163-166.
Mohamed, E. A. H., Lim, C. P., Ebrika, O. S., Asmawi, M. Z., Sadikun, A., Yam, M. F. 2011. Toxicity Evaluation of a Standardised 50% Ethanol Extract of Orthosiphon Stamineus. Journal of Ethnopharmacology. 133: 358-363.
Huong, N. T. T., Matsumoto, K., Nham, N. T., Quang, N. H., Duc, N. M., Yamasaki, K., & Watanabe, H. 1997. Effect of Vietnamese Ginseng on the phagocytosis in Vitro and in Vivo. Phytomedicine. 4(4): 341-346.
Shwter, Abdrabuh N. et al. 2014. Chemoprevention of Colonic Aberrant Crypt Foci by Gynura Procumbens in Rats. Journal of Ethnopharmacology. 151(3): 1194-1201.
Adeneye, A. A., Ajagbonna, O. P.,Adeleke, T. I., Bello, S. O. 2006. Preliminary Toxicity and Phytochemical Studies of the Stem Bark Aqueous Extract of Musanga Cecropioides in Rats. Journal of Ethnopharmacology. 105: 374-379.
Hilaly, J., El. Israili, Z., Lyoussi, B. 2004. Acute and Chronic Toxicological Studies of Ajuga Iva in Experimental Animals. Journal of Ethnopharmacology. 91: 43-50.
Das, M., Dasgupta, S. C., Gomes, A. 1998. Immunomodulatory and Anti-Neoplastic Activity of Common Indian Toad (Bufo Melanostictus Schneider) Skin Extract. Indian J Pharmacol. 30: 311-317.
Jayathirtha, M. G., Mishra, S. H. 2004. Preliminary Immunomodulatory Activities of Methanol Extracts of Eclipta Alba And Centella Asiatica. Phytomedicine; 11: 361-365.
Dashputre, N. L., & Naikwade, N. S. 2010. Immunomodulatory Activity of Abutilon Indicum linn on Albino Mice. 1(3): 178-184.
Kaewseejan, N., & Siriamornpun, S. 2015. Bioactive Components and Properties of Ethanolic Extract and Its Fractions from Gynura Procumbens Leaves. Industrial Crops and Products. 74: 271-278.
Kolodziej, H., & Kiderlen, A. F. 2005. Antileishmanial Activity and Immune Modulatory Effects of Tannins and Related Compounds on Leishmania Parasitised RAW 264.7 Cells. Phytochemistry. 66(17): 2056-71.
Soehnlein, O. and Lindbom, L. 2010. Phagocyte Partnership during the Onset and Resolution of Inflammation. Nat. Rev. Immunol. 10: 427-439.
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