ISOLATION AND SCREENING OF ENDOSULFANDEGRADING FUNGI FROM SOIL

Authors

  • Jericho Victor Lagasca Mercado Chemical Engineering Department, Gokongwei College of Engineering, De La Salle University-Manila
  • Josephine Borja Chemical Engineering Department, Gokongwei College of Engineering, De La Salle University-Manila
  • Susan Gallardo Chemical Engineering Department, Gokongwei College of Engineering, De La Salle University-Manila

DOI:

https://doi.org/10.11113/aej.v1.15320

Abstract

Fungal colonies capable of tolerating and degrading endosulfan were enriched and isolated from soil with history of endosulfan application. The microorganisms were enriched using technical-grade endosulfan with gradual increase in concentration from 10 to 100 mgl-1, each enrichment run lasting for 210 h. Four (4) fungal isolates, labeled as P701, P702, P601 and P801, were obtained by successive plating of enriched colonies. The isolates were screened based on α- and β-endosulfan degradation in nutrient media with endosulfan as sole-carbon source (NM-SCS) and as sole-sulfur source (NM-SSS). Results indicated that P601 grown in the NM-SSS exhibited the highest endosulfan degradation after 14 d (93.85% for α-endosulfan and 90.29% for β-endosulfan). Isolate P601 was identified as Candida tropicalis using the API C AUX yeast identification system.

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Published

2012-09-10

Issue

Vol. 1 No. 1 (2012): June 2012 Part B

Section

Chemical Engineering

How to Cite

ISOLATION AND SCREENING OF ENDOSULFANDEGRADING FUNGI FROM SOIL. (2012). ASEAN Engineering Journal, 1(1), 5-13. https://doi.org/10.11113/aej.v1.15320