Purification and Characterization of Extracellular Polymeric Substances (EPS) with Antimicrobial Properties from s Marine Epibiotic Pseudoalteromonas sp.


  • Ainul Masliana Ismail Faculty of Biosciences and Bioengineering, Universiti Teknologi Malaysia
  • Wan Nur Atirah Wan Azemin Faculty of Biosciences and Bioengineering, Universiti Teknologi Malaysia
  • Azmi Rani Brackishwater Culture Research Centre, Department of Fisheries, Ministry of Agriculture and Agro–based Industry, Malaysia
  • Mohd Shahir Shamsir Faculty of Biosciences and Bioengineering, Universiti Teknologi Malaysia




Extracellular polymeric substances, pseudoalteromonas, fish mucus, colorimetric analysis, antimicrobial activity, antimicrobial components


We present a study to purify and characterize the extracellular polymeric substances (EPS) produced by Pseudoalteromonas sp.isolated from fish epidermal surface. The purified EPS was recovered from the culture supernatant by using cold ethanol precipitation and dialysis method. Colorimetric analysis of EPS revealed that it was primarily composed of proteins, 0.0251g/L, carbohydrates, 0.0561g/L and uronic acid, 0.0217 g/L. The EPS also exhibited high emulsification activity when being tested with hydrophobic substances such as paraffin oil, (94.5 % and 79.4%), kerosene, (56.1% and 37.2%) and Tween 80 (46.6% and 32.9%). In addition, the EPS demonstrated discernible antimicrobial activity against Gram–negative bacteria like E.coli, P.aeruginosa and E.aerogenes. The SDS–PAGE profile revealed up to eight types of unknown proteins were detected within the EPS, with the molecular weight ranging from 15.486 kDa to 113.058 kDa. The results of the study would contribute to the elucidation of antimicrobial components and its mechanism inPseudoalteromonas sp.


Bernadsky, G. and E. Rosenberg. 1992. Drag-Reducing Properties of

Bacteria from the Skin Mucus of the Cornetfish (Fistularia commersonii.

Microbial Ecology. 24(1): 63–76.

Bhaskar, P. V. and N. B. Bhosle. 2005. Microbial Extracellular

Polymeric Substances in Marine Biogeochemical Processes. Current

Science. 88(1): 45–53.

Bramhachari, P. V., P. B. Kishor, et al. 2007. Isolation and

Characterization of Mucous Exopolysaccharide (EPS) Produced by

Vibrio Furnissii Strain VB0S3. Journal of Microbiology and

Biotechnology. 17(1): 44–51.

Fazio, S. A., D. J. Uhlinger, et al. 1982. Estimations of Uronic Acids as

Quantitative Measures of Extracellular and Cell Wall Polysaccharide

Polymers from Environmental Samples. Applied Environmental

Microbiology. 43(5): 1151–1159.

Flemming, H. C. and J. Wingender. 2001. Relevance of Microbial

Extracellular Polymeric Substances (Epss)--Part II: Technical Aspects.

Water Science and Technology. 43(6): 9–16.

Longeon, A., J. Peduzzi, et al. 2004. Purification and Partial

Identification of Novel Antimicrobial Protein from Marine Bacterium

Pseudoalteromonas Species Strain X153. Marine Biotechnology. 6(6):