Purification and Characterization of Extracellular Polymeric Substances (EPS) with Antimicrobial Properties from s Marine Epibiotic Pseudoalteromonas sp.
Keywords:Extracellular polymeric substances, pseudoalteromonas, fish mucus, colorimetric analysis, antimicrobial activity, antimicrobial components
AbstractWe present a study to purify and characterize the extracellular polymeric substances (EPS) produced by Pseudoalteromonas sp.isolated from fish epidermal surface. The purified EPS was recovered from the culture supernatant by using cold ethanol precipitation and dialysis method. Colorimetric analysis of EPS revealed that it was primarily composed of proteins, 0.0251g/L, carbohydrates, 0.0561g/L and uronic acid, 0.0217 g/L. The EPS also exhibited high emulsification activity when being tested with hydrophobic substances such as paraffin oil, (94.5 % and 79.4%), kerosene, (56.1% and 37.2%) and Tween 80 (46.6% and 32.9%). In addition, the EPS demonstrated discernible antimicrobial activity against Gramâ€“negative bacteria like E.coli, P.aeruginosa and E.aerogenes. The SDSâ€“PAGE profile revealed up to eight types of unknown proteins were detected within the EPS, with the molecular weight ranging from 15.486 kDa to 113.058 kDa. The results of the study would contribute to the elucidation of antimicrobial components and its mechanism inPseudoalteromonas sp.
Bernadsky, G. and E. Rosenberg. 1992. Drag-Reducing Properties of
Bacteria from the Skin Mucus of the Cornetfish (Fistularia commersonii.
Microbial Ecology. 24(1): 63â€“76.
Bhaskar, P. V. and N. B. Bhosle. 2005. Microbial Extracellular
Polymeric Substances in Marine Biogeochemical Processes. Current
Science. 88(1): 45â€“53.
Bramhachari, P. V., P. B. Kishor, et al. 2007. Isolation and
Characterization of Mucous Exopolysaccharide (EPS) Produced by
Vibrio Furnissii Strain VB0S3. Journal of Microbiology and
Biotechnology. 17(1): 44â€“51.
Fazio, S. A., D. J. Uhlinger, et al. 1982. Estimations of Uronic Acids as
Quantitative Measures of Extracellular and Cell Wall Polysaccharide
Polymers from Environmental Samples. Applied Environmental
Microbiology. 43(5): 1151â€“1159.
Flemming, H. C. and J. Wingender. 2001. Relevance of Microbial
Extracellular Polymeric Substances (Epss)--Part II: Technical Aspects.
Water Science and Technology. 43(6): 9â€“16.
Longeon, A., J. Peduzzi, et al. 2004. Purification and Partial
Identification of Novel Antimicrobial Protein from Marine Bacterium
Pseudoalteromonas Species Strain X153. Marine Biotechnology. 6(6):
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