STATISTICAL OPTIMIZATION OF GELATIN IMMOBILISATION ON MODIFIED SURFACE PCL MICROCARRIER TO IMPROVE PCL MICROCARRIER COMPATIBILITY

Authors

  • Nurhusna Samsudin Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia, P.O. Box 10, 50728 Kuala Lumpur, Malaysia
  • Yumi Zuhanis Has-Yun Hashim International Institute for Halal Research and Training (INHART), Level 3, Kulliyyah of ICT Building, International Islamic University Malaysia, P.O. Box 50728, Kuala Lumpur, Malaysia
  • Mohd Azmir Arifin Faculty of Engineering Technology, University Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang, Malaysia
  • Maizirwan Mel Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia, P.O. Box 10, 50728 Kuala Lumpur, Malaysia
  • Hamzah Mohd. Salleh Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia, P.O. Box 10, 50728 Kuala Lumpur, Malaysia
  • Norshariza Nordin Genetics and Regenerative Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, Malaysia
  • Iis Sopyan Department of Manufacturing and Material Engineering, Kulliyyah of Engineering, International Islamic University Malaysia, P.O. Box 10, 50728 Kuala Lumpur, Malaysia
  • Dzun Noraini Jimat Department of Manufacturing and Material Engineering, Kulliyyah of Engineering, International Islamic University Malaysia, P.O. Box 10, 50728 Kuala Lumpur, Malaysia

DOI:

https://doi.org/10.11113/jt.v79.10417

Keywords:

Microcarrier, gelatin immobilization, polycaprolactone, statistical optimization

Abstract

Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excellent mechanical properties and degradation kinetics that suit various applications, but its non-polar hydrocarbon moiety renders it sub-optimal for cell attachment. In this present study, the aim was to improve biocompatibility of PCL microcarrier by introducing oxygen functional group via ultraviolet irradiation and ozone aeration (UV/O3 system) to allow covalent immobilization of gelatin on the PCL microcarrier surface. Respond surface methodology was used as a statistical approach to optimized parameters that effect the immobilization of gelatin. The parameters used to maximized amount of gelatin immobilize were the mol ratio of COOH:EDAC, NHS concentration and gelatin concentration. The optimum conditions for maximum amount of gelatin (1797.33 µg/g) on the surface of PCL were as follows: 1.5 of COOH:EDAC ratio, 10 mM NHS concentration and, 80 mg/ml gelatin. The result shows that gelatin coated PCL microcarrier promote more and rapid cell adhesion with density of  as compared to raw PCL microcarrier (  and UV/O3 treated PCL microcarrier ( . Therefore, immobilization of gelatin with optimized parameters onto PCL microcarrier improved biocompatibility of PCL microcarrier.

Author Biography

  • Nurhusna Samsudin, Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia, P.O. Box 10, 50728 Kuala Lumpur, Malaysia

    FACULTY OF ENGINEERING, IIUM
    GOMBAK 53100 KL

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Published

2017-08-28

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Section

Science and Engineering

How to Cite

STATISTICAL OPTIMIZATION OF GELATIN IMMOBILISATION ON MODIFIED SURFACE PCL MICROCARRIER TO IMPROVE PCL MICROCARRIER COMPATIBILITY. (2017). Jurnal Teknologi (Sciences & Engineering), 79(6). https://doi.org/10.11113/jt.v79.10417