STATISTICAL OPTIMIZATION OF GELATIN IMMOBILISATION ON MODIFIED SURFACE PCL MICROCARRIER TO IMPROVE PCL MICROCARRIER COMPATIBILITY

Authors

  • Nurhusna Samsudin Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia, P.O. Box 10, 50728 Kuala Lumpur, Malaysia
  • Yumi Zuhanis Has-Yun Hashim International Institute for Halal Research and Training (INHART), Level 3, Kulliyyah of ICT Building, International Islamic University Malaysia, P.O. Box 50728, Kuala Lumpur, Malaysia
  • Mohd Azmir Arifin Faculty of Engineering Technology, University Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang, Malaysia
  • Maizirwan Mel Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia, P.O. Box 10, 50728 Kuala Lumpur, Malaysia
  • Hamzah Mohd. Salleh Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia, P.O. Box 10, 50728 Kuala Lumpur, Malaysia
  • Norshariza Nordin Genetics and Regenerative Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, Malaysia
  • Iis Sopyan Department of Manufacturing and Material Engineering, Kulliyyah of Engineering, International Islamic University Malaysia, P.O. Box 10, 50728 Kuala Lumpur, Malaysia
  • Dzun Noraini Jimat Department of Manufacturing and Material Engineering, Kulliyyah of Engineering, International Islamic University Malaysia, P.O. Box 10, 50728 Kuala Lumpur, Malaysia

DOI:

https://doi.org/10.11113/jt.v79.10417

Keywords:

Microcarrier, gelatin immobilization, polycaprolactone, statistical optimization

Abstract

Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excellent mechanical properties and degradation kinetics that suit various applications, but its non-polar hydrocarbon moiety renders it sub-optimal for cell attachment. In this present study, the aim was to improve biocompatibility of PCL microcarrier by introducing oxygen functional group via ultraviolet irradiation and ozone aeration (UV/O3 system) to allow covalent immobilization of gelatin on the PCL microcarrier surface. Respond surface methodology was used as a statistical approach to optimized parameters that effect the immobilization of gelatin. The parameters used to maximized amount of gelatin immobilize were the mol ratio of COOH:EDAC, NHS concentration and gelatin concentration. The optimum conditions for maximum amount of gelatin (1797.33 µg/g) on the surface of PCL were as follows: 1.5 of COOH:EDAC ratio, 10 mM NHS concentration and, 80 mg/ml gelatin. The result shows that gelatin coated PCL microcarrier promote more and rapid cell adhesion with density of  as compared to raw PCL microcarrier (  and UV/O3 treated PCL microcarrier ( . Therefore, immobilization of gelatin with optimized parameters onto PCL microcarrier improved biocompatibility of PCL microcarrier.

Author Biography

  • Nurhusna Samsudin, Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia, P.O. Box 10, 50728 Kuala Lumpur, Malaysia

    FACULTY OF ENGINEERING, IIUM
    GOMBAK 53100 KL

References

Brun-Graeppi, A., Richard, C., Michel, S., Daniel, M. & Otto-Wilhelm. 2001. Cell Microcarriers and Microcapsules of Stimuli-responsive Polymers. Journal Of Controlled Release. 149: 209-224.

Tan, H., Huang, D., Lao, L., & Gao, C. 2009. RGD Modified PLGA/Gelatin Microspheres as Microcarriers for Chondrocyte Delivery. Journal of Biomedical Materials Research. 91: 228-238.

Nikam, V. K., Gudsoorkar, V. R., Hiremath, S. N., Dolas, R. T., & Kashid, V. A. 2012. Microspheres - A Novel Drug Delivery System. International Journal of Pharmaceutical and Chemical Science. 1: 113-128.

Ma, Z., Gao, C., Ji, J., & Shen, J. 2002. Protein Immobilisation on the Surface of Poly-L-Lactic Acid Films for Improvement of Cellular Interactions. European Polymer Journal. 38: 2279-2284.

Yuan, S., Xiong, G., Roguin, A., & Choong, C. 2012. Immobilisation of Gelatin onto Poly(Glycidyl Methacrylate)-grafted Polycaprolactone Substrates for Improved Cell-material Interactions. Biointerphases. 7(30): 1-4.

Khan, W., Kapoor, M., & Kumar, N. 2007. Covalent Attachment of Proteins to Functionalized Polypyrrole-coated Metallic Surfaces for Improved Biocompatibility. ActaBiomaterialia. 3: 541-549.

Zhu, Y., Gao, C., Liu, X., & Shen, J. 2002. Surface Modification of Polycaprolactone Membrane via Aminolysis and Biomacromolecule Immobilisation for Promoting Cytocompatibility of Human Endothelial Cells. Biomacromolecules. 3(6): 1312-9. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/12425670.

Mojarradi, H. 2011. Coupling of Substances Containing a Primary Amine to Hyaluronan via Carbodiimide-mediated Amidation. Uppsala University Publications. 1-44.

Wahid, Z., & Nadir, N. 2013. Improvement of on Factor at a Time Through Design of Experiment. World Appl. Sci. J. 21(Mathematical Application in Engineering): 56-61.

Hinkelmann, K., and Kempthorne, O. 2008. Design of Experiment: Introduction to Experimental Design. vol 2. John Wiley and Sons, New York.

Prockop and Udenfriend. 1960. A Specific Method for the Analysis of Hydroxyproline in Tissues and Urine. Anal. Bicchem. 1(3): 228-239.

Hermanson, G. T. 2008. Bioconjugate Techniques. 2nd edd. San Diego: Academic Press.

Gomathi, N., & Neogi, S. 2009. Surface Modification of Polypropylene Using Argon Plasma: Statistical Optimization of the Process Variables. Applied Surface Science. 255(17): 7590-7600.

Kemala, T., Budianto, E., & Soegiyono, B. 2012. Preparation and Characterisation of Microspheres Based on Blend of Poly(Lactic Acid) and Poly(E-Caprolactone) with Poly(Vinyl Alcohol) as Emulsifier. Arabian Journal of Chemistry. 5(1): 103-108.

Goh, T. K.-P., Zhang, Z.-Y., Chen, A. K.-L., Reuveny, S., Choolani, M., Chan, J. K. Y., & Oh, S. K.-W. 2013. Microcarrier Culture for Efficient Expansion and Osteogenic Differentiation of Human Fetal Mesenchymal Stem Cells. BioResearch Open Access. 2(2): 84-97.

Yang, Y., Porte, M., Marmey, P., El Hai, A.J., Amedee, J., & Baquey, C. 2003. Covalent Bonding of Collagen on Poly(L-Lactic Acid) by Gamma Irradiation. Nuclear Instrumentation Methods. 207: 165-174.

Arifin, M. A., Mel, M., Samsudin, N., Hashim, Y. Z. H.-Y., Salleh, H. M., Sopyan, I. and Nordin, N. 2015. Ultraviolet/ozone Treated Polystyrene Microcarriers for Animal Cell Culture. J. Chem. Technol. Biotechnol. 90: 2607-2619.

Downloads

Published

2017-08-28

Issue

Vol. 79 No. 6: September 2017

Section

Science and Engineering

License

Copyright of articles that appear in Jurnal Teknologi belongs exclusively to Penerbit Universiti Teknologi Malaysia (Penerbit UTM Press). This copyright covers the rights to reproduce the article, including reprints, electronic reproductions, or any other reproductions of similar nature.

How to Cite

STATISTICAL OPTIMIZATION OF GELATIN IMMOBILISATION ON MODIFIED SURFACE PCL MICROCARRIER TO IMPROVE PCL MICROCARRIER COMPATIBILITY. (2017). Jurnal Teknologi, 79(6). https://doi.org/10.11113/jt.v79.10417