• Noorrasyidah Mohd Sarmin Industrial Biotechnology Research Centre, SIRIM Berhad, 1 Persiaran Dato’ Menteri, P.O Box 7035, 40700 Shah Alam, Selangor, Malaysia
  • Shazana Azfar Razali Department of Engineering and Build Environment, National University of Malaysia, 43600 UKM, Bangi Selangor, Malaysia
  • Masturah Markom Department of Engineering and Build Environment, National University of Malaysia, 43600 UKM, Bangi Selangor, Malaysia




Expanded Liquid Organic Solution (DELOS), Supercritical CO2, Citrus Hystrix L. Oil (CHO), Response surface methodology (RSM), microencapsulation


Response surface methodology (RSM) was used to optimise the microencapsulation process of Citrus Hystrix L. Oil (CHO) by depressurization of an expanded liquid organic solution (DELOS) sub and supercritical CO2. The particle size and yield (%) was studied with considered to three key factors variables including pressure (30-80 bar), temperature (40-60ËšC) and resident time (10-60 minutes) on the microencapsulation process. The optimum result from RSM is 549.4 nanometer for the particle size and 38.193% yield at optimum pressure 54.14bar, temperature 59.65ËšC and resident time 58 minutes. The results clearly show that the interactions between pressure, temperature and time have a significant effect on the microencapsulation process. Thus, the microencapsulated formulation has potential to be applied to other volatile compounds.


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