EFFECT OF REACTION CONDITIONS ON THE SYNTHESIS OF CYCLODEXTRIN (CD) BY USING IMMOBILIZED ENZYME

Authors

  • Suhaily Suhaimi Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300, Gambang, Pahang, Malaysia
  • Rohaida Che Man Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300, Gambang, Pahang, Malaysia
  • Natassha Jamil Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300, Gambang, Pahang, Malaysia
  • Zatul Iffah Mohd Arshad Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300, Gambang, Pahang, Malaysia
  • Shalyda Md Shaarani Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300, Gambang, Pahang, Malaysia
  • Siti Zubaidah Sulaiman Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300, Gambang, Pahang, Malaysia
  • Siti Kholijah Abdul Mudalip Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300, Gambang, Pahang, Malaysia
  • Aizi Nor Marzila Ramli Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300, Gambang, Pahang, Malaysia

DOI:

https://doi.org/10.11113/jt.v80.11707

Keywords:

Cyclodextrin, cyclodextrin glucanotransferase, enzyme immobilization, hollow fiber membrane

Abstract

The production of cyclodextrin (CD) over the years has been increasing due to the numerous applications in industries such as in food, cosmetic, pharmaceutical and agricultural industries. However, cyclodextrin glucanotransferase (CGTase) which involved in the enzymatic reaction on the production of CD is unstable and easily denatured at extreme conditions resulted in low CD production. Hence, the enzyme immobilization technique is introduced to overcome these problems and subsequently increase the production of CD. In the present study, the CGTase was immobilized on hollow fiber membrane to increase the production of CD during the reaction. The effect of reaction conditions (types of starch, concentration of starch, temperature and pH) of the immobilized enzyme on the production of CD were investigated. Among the three types of starch tested, the soluble potato starch was the most suitable substrate for the production of CD with 4.13 mg/mL. In addition, by using 3% (w/v) of the soluble potato starch, the production of CD was 5.22 mg/mL . The optimal reaction temperature and pH were found to be at 40°C and pH 6 with 5.21 mg/mL and 4.62 mg/ml of CD, respectively. The immobilized enzyme exhibited a 1.3-3-fold increase in CD production compared to the free enzyme. Therefore, the hollow fiber membrane is suitable to be used as a support for enzyme immobilization with the high production of CD.

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Published

2018-02-26

Issue

Vol. 80 No. 3: May 2018

Section

Science and Engineering

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How to Cite

EFFECT OF REACTION CONDITIONS ON THE SYNTHESIS OF CYCLODEXTRIN (CD) BY USING IMMOBILIZED ENZYME. (2018). Jurnal Teknologi, 80(3). https://doi.org/10.11113/jt.v80.11707