WOUND HEALING PROPERTIES OF SWIETENIA MAHAGONI SEED EXTRACTED USING SCCO2: AN IN VITRO STUDY
DOI:
https://doi.org/10.11113/jt.v81.12195Keywords:
Wound healing, Swietenia mahagoni, supercritical carbon dioxideAbstract
Swietenia mahagoni seed has been traditionally used as a wound healing agent. The aim of this study was to investigate the in vitro healing properties of Swietenia mahagoni seed extract. A supercritical carbon dioxide (SCCO2) extract of Swietenia mahagoni seeds was tested regarding its ability to stimulate the growth of human skin fibroblasts (HSF 1184) in vitro using the colorimetric methylthiazol tetrazolium (MTT) assay and scratch assay in a selected range of extract concentrations. The results show that the Swietenia mahagoni seed extract at a concentration of 0.01 mg/mL was able to stimulate the growth of human skin fibroblasts. The scratch assay showed that all of the extracts led to a significantly higher percentage of wound closure compared to the control. The wound healing capabilities of Swietenia mahagoni seed extract may be due to its fibroblast stimulation activity, which may promote repair of the wounded dermis. Thus, it may serve as a good herbal component to incorporate into products designed to promote wound healing.
References
Ekpo, M., Mbagwu, H., Jackson, C. and Eno, M. 2011. Antimicrobial and Wound Healing Activities of Centrosema pubescens (Leguminosae). Journal of Physics and Chemistry of Solids. 1: 1-6.
Joseph, B and Justin, R. S. 2011. A Comparative Study on Various Properties of Five Medicinally Important Plants. International Journal of Pharmacology. 7(2): 206-211.
Adetutu, A., Morgan, W. A. and Corcoran, O. 2011. Ethnopharmacological Survey and in vitro Evaluation of Wound-Healing Plants Used in South-Western Nigeria. Journal of Ethnopharmacology. 137: 50-56.
Balekar, N., Gangadhar, K. N., Nakpheng, T., Jehtae, K., and Srichana, T. 2012. Evaluation of The Wound Healing Potential of Wedelia trilobata (L) Leaves. Journal of Ethnopharmacology. 141: 817-824.
Chen, Y. Y., Wang, X. N., Fan, C. Q., Yin, S. and Yue, J. M. 2007. Swiemahogins A and B, Two Novel Limnoids from Swietenia mahogany. Tetrahedron Letters. 48: 7480-7484.
Bascal, K., Chavez, L., Diaz, I., Espina, S., Javillo, J., Manzanilla, H., Motalban, J., Panganiban, C., Rodriguez, A., Sumpaico, C., Talip, B., and Yap, S. 1997. The Effect os Swietenia mahagoni (Mahogany) Seed Extract on Indomethacin-induced Gastric Ulcers in Female Sprague-dawley Rats. ActaMed. Philipp. 3: 127-139.
Guevara, A.P., Apilado, A., Sakurai, H., Kozuka, M. and Tokuda, H. 1996. Anti-Inflammatory, Antimutagenic and Antitumor Promoting Activities of Mahogany Seeds, Swietenia macrophylla (Meliaceae). Philippine Journal of Science. 125: 271-278.
Hartati, Salleh, M. I., Ramdan, A., Aziz A. A. and Yunus, M. A. 2014. Supercritical Fluid Extraction of Swietenia mahagoni Seed: Antioxidant and Antimicrobial Activities. Jurnal Teknologi (Science and Engineering). 67(4): 59-62.
Alrdahe, S. S., Abdulla, M. A., Razak, S. A., Kadir, F. A. and Hassandarvish, P. 2010. Gastroprotective Activity of Swietenia Mahagoni Seed Extract on Ethanol-Induced Gastric Mucosal Injury in Rats. World Academyca of Science, Engineering and Technology. 67: 883-887.
Ali, M. A., Sayeed, M. A., Islam, M. S., Yeasmin, M. S., Khan, G. R. M. A. M. and Ida, I. M. 2011. Physicochemical and Antimicrobial Properties of Trichosanthes anguina and Swietenia mahagoni seeds. Bull.Chem. Soc. Ethiop. 25(3): 427-436.
Ranzato, E., Martinotti, S., and Burlando, B. 2011. Wound Healing Properties of Jojoba Liquis Wax: An in Vitro Study. Journal of Ethnopharmacology. 134: 443-449.
Fronza, M., Heinzmann, B., Hamburger, M., Laufer, S. and Merfort, I. 2009. Determination of the Wound Healing Effect of Calendula Extract Using the Scratch Assay With 3T3 Fibroblasts. Journal of Ethnopharmacology. 126: 463-467.
Casas, I., Mantell, C., Rodroguez, M., Torres, A., Macias, F.A. and De la Ossa, E.J.M. 2008. Supercritical Fluid Extraction of Bioactive Compounds from Sunflower Leaves with Carbon dioxide and Water on a Pilot Plant Scale. J. Supercritical Fluids. 45: 37-42.
Liu, J., Han, B., Liu, G.Li., He, .J. and Yang, G. 2001. Solubility of the Non-Ionic Surfactant Tetraethylene Glycol N-Laurel Ether in Supercritical CO2 with N-Pentanol, Fluid Phase Equilibrium. 15: 247-254.
Sahgal, G., Ramanathan, S., Sasidharan, S., Mordi, M. N., Ismail, S. and Mansor, S. M. 2009. Phytochemical and Antimicrobial Activity of Swietenia mahagoni Crude Methanolic Seed Extract. Tropical Biomedicine. 26 (3): 274-279.
Nagalakshmi, M. A. H., Thangadurai, D., Rao, D. M. and Pullailah, T. 2001. Phytochemical and Antimicrobial Study of Churasia tabularis Leaves. Fitoterapia. 72: 62-64.
Barile, F. A., Dierick, P. J, and Kristen, U. 1994. In Vitro Cytotoxicity Testing for Prediction of Acute Human Toxicity. Journal Cell Biology and Toxicology. 10: 155-162.
Wang, Z., Pan, Z., Ma, H. and Atungulu, G. G. 2011. Extract of Phenolics from Pomegranate Peels. The Open Food Science Journal. 5: 17-25.
Schmidt, C., Fronza, M., Goettert, M., Geller, F., Luik, S., Flores, E. M. M., Bittencourt, Zanetti, G. D., Heinzmann, B. M., Laufer, S. and Merfort, I. 2009. Biological Studies on Brazilian Plants used in Wound Healing. Journal of Ethnopharmacology. 122: 523-532.
Downloads
Published
Issue
Section
License
Copyright of articles that appear in Jurnal Teknologi belongs exclusively to Penerbit Universiti Teknologi Malaysia (Penerbit UTM Press). This copyright covers the rights to reproduce the article, including reprints, electronic reproductions, or any other reproductions of similar nature.
