• M. Z. Zainuddin Faculty of Dentistry, UiTM Sungai Buloh Campus, Jalan Hospital, 47000 Sungai Buloh, Selangor Darul Ehsan, Malaysia
  • Kamisah Y. Departments of Anatomy and Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, W. P., Kuala Lumpur, Malaysia
  • Farida H. Human Biology Department, School of Medicine, International Medical University, Jalan Jalil Perkasa 19, 57000 Bukit Jalil, W. P. Kuala Lumpur, Malaysia
  • Faizah O. Departments of Anatomy and Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, W. P., Kuala Lumpur, Malaysia




Morinda citrifolia, exercise, endurance test, fatigue, swimming


Morinda citrifolia (MC) is a herb that is famous for its various medicinal properties. However, there has been little research on its effect on increased stamina and physical fatigue reduction. The purpose of this study was to determine the effect of high-dose oral intake of 50% ethanol MC leaf extract on exercise performance and physical fatigue in an animal model. Thirty female Sprague-Dawley rats were divided into five groups; Control (C); Sedentary (S); Exercise (EX); MC leaf extract 1000 mg/kg/day (MC); MC leaf extract 1000 mg/kg/day with Exercise (MC+EX). The endurance test showed a significant difference in endurance time between EX and MC groups, whereby intake of MC leaf extract without daily exercise lowered endurance level by 68.75%. These findings correlated with serum fatigue indicator analysis whereby significant increases in CK levels of MC group, and LDH levels in MC and MC+EX groups were noted when compared to other groups. Electron microscopy analysis on cardiac muscle tissue showed ultrastructural injuries in muscle fibers of the EX, MC and MC+EX groups whereas injuries to the gastrocnemius myofibres were not so prominent. Based on the study findings, it can be concluded that the consumption of 1000 mg/kg/day 50% ethanol MC extract disturbed internal physiology and resulted in injury to the muscle tissues thus lowering rats endurance capability. This shows that a high dose has unfavourable effects on an animal model while a lower dose may be more appropriate.


Jason, L. A., et al. 2010. What is fatigue? Pathological and Nonpathological Fatigue. PM R. 2(5): 327-31.

Fuhrer, R. and S. Wessely. 1995. The Epidemiology of Fatigue and Depression: A French Primary-Care Study. Psychol Med. 25(5): 895-905.

Bensing, J. M., R. L. Hulsman, and K. M. Schreurs. 1999. Gender Differences in Fatigue: Biopsychosocial Factors Relating to Fatigue in Men and Women. Med Care. 37(10): 1078-83.

Ballenger, J. C., et al. 2001. Consensus Statement on Generalized Anxiety Disorder from the International Consensus Group on Depression and Anxiety. J Clin Psychiatry. 62 (Suppl 11): 53-8.

Demyttenaere, K., J. De Fruyt, and S. M. Stahl. 2005. The Many Faces of Fatigue in Major Depressive Disorder. Int J Neuropsychopharmacol. 8(1): 93-105.

Morin, C. M. and R. Benca. 2012. Chronic Insomnia. Lancet. 379(9821): 1129-41.

Tanaka, M., et al. 2008. Effects of (-) -epigallocatechin Gallate in Liver of an Animal Model of Combined (Physical and Mental) Fatigue. Nutrition. 24(6): 599-603.

Xu, C., et al. 2013. Effects of Oat Beta-glucan on Endurance Exercise and Its Anti-fatigue Properties in Trained Rats. Carbohydr Polym. 92(2): 1159-65.

Wang, J., et al. 2014. The Effective Mechanism of the Polysaccharides from Panax Ginseng on Chronic Fatigue Syndrome. Arch Pharm Res. 37(4): 530-8.

Afari, N. and D. Buchwald. 2003. Chronic Fatigue Syndrome: A Review. Am J Psychiatry. 160(2): 221-36.

Huang, L. Z., et al. 2011. Bioactivity-guided Fractionation for Anti-fatigue Property of Acanthopanax Senticosus. J Ethnopharmacol. 133(1): 213-9.

Wang, M. Y., et al. 2002. Morinda citrifolia (Noni): A Literature Review and Recent Advances in Noni Research. Acta Pharmacol Sin. 23(12): 1127-41.

Chan-Blanco, Y., et al. 2006. The Noni Fruit (Morinda citrifolia L.): A Review of Agricultural Research, Nutritional and Therapeutic Properties. Journal of Food Composition and Analysis. 19(6-7): 645-654.

Wang, M. Y. and C. Su. 2001. Cancer Preventive Effect of Morinda citrifolia (Noni). Ann N Y Acad Sci. 952: 161-8.

Mandukhail, S. U., N. Aziz, and A. H. Gilani. 2010. Studies on Antidyslipidemic Effects of Morinda Citrifolia (Noni) Fruit, Leaves and Root Extracts. Lipids Health. Dis, 9: 88.

Bhatia, R., K. Thapliyal, and D. Kumar. 2015. Phytochemical and Therapeutic Aspects of Morinda citrifolia L.(Noni Plant): A Review. Research Journal of Pharmacognosy and Phytochemistry. 7(3): 167-174.

Furusawa, E., et al. 2003. Antitumour Potential of a Polysaccharide-rich Substance from the Fruit Juice of Morinda citrifolia (Noni) on Sarcoma 180 Ascites Tumour in Mice. Phytother Res. 17(10): 1158-64.

Saludes, J. P., et al. 2002. Antitubercular Constituents from the Hexane Fraction of Morinda Citrifolia Linn. (Rubiaceae). Phytother Res. 16(7): 683-5.

Zin, Z. M., et al. 2006. Antioxidative activities of chromatographic fractions obtained from root, fruit and leaf of Mengkudu (Morinda citrifolia L.). Food Chemistry. 94(2): 169-178.

Zin, Z.M., A. Abdul-Hamid, and A. Osman. 2002. Antioxidative Activity of Extracts from Mengkudu (Morinda citrifolia L.) Root, Fruit and Leaf. Food Chemistry. 78(2): 227-231.

Rasal, V. P., et al. 2008. Wound Healing and Antioxidant Activities of Morinda Citrifolia Leaf Extract in Rats. Iranian Journal of Pharmacology & Therapeutics. 7(1): 49-52.

Nayak, B. S., S. Sandiford, and A. Maxwell. 2009. Evaluation of the Wound-healing Activity of Ethanolic Extract of Morinda citrifolia L. Leaf. Evidence-Based Complementary and Alternative Medicine. 6(3): 351-356.

West, B. J., et al. 2007. Safety Tests and Antinutrient Analyses of Noni (Morinda citrifolia L.) Leaf. J Sci Food Agric. 87(14): 2583-8.

Balci, S. S. and H. Pepe. 2012. Effects of Gender, Endurance Training and Acute Exhaustive Exercise on Oxidative Stress in the Heart and Skeletal Muscle of the Rat. Chin J Physiol. 55(4): 236-44.

Yu, F. R., et al. 2010. Effects of a Flavonoid Extract from Cynomorium Songaricum on the Swimming Endurance of Rats. Am J Chin Med. 38(1): 65-73.

Matsumoto, K., et al. 1996. An Adjustable-current Swimming Pool for the Evaluation of Endurance Capacity of Mice. J Appl Physiol. 81(4): 1843-9.

Mohamad Shalan, N. A., N. M. Mustapha, and S. Mohamed. 2016. Morinda Citrifolia Leaf Enhanced Performance by Improving Angiogenesis, Mitochondrial Biogenesis, Antioxidant, Anti-Inflammatory & Stress Responses. Food Chem. 212: 443-52.

Huang, C. C., et al. 2012. Triterpenoid-Rich Extract from Antrodia camphorata Improves Physical Fatigue and Exercise Performance in Mice. Evid Based Complement Alternat Med. 364741.

Rose, A. J. and E. A. Richter. 2005. Skeletal Muscle Glucose Uptake during Exercise: How is it Regulated? Physiology (Bethesda). 20: 260-70.

Yeh, T.-S., et al. 2014. Angelica Sinensis Improves Exercise Performance and Protects Against Physical Fatigue in Trained Mice. Molecules. 19(4): 3926-3939.

Yeh, T. S., et al. 2014. Astragalus Membranaceus Improves Exercise Performance and Ameliorates Exercise-induced Fatigue in Trained Mice. Molecules. 19(3): 2793-807.

Marler, T. E. and J. Yang. 2013. Risk of Aluminum Exposure from Noni (Morinda Citrifolia L.) Leaf Products. Economic Botany. 67(3): 203-209.

Paul, A. C. and N. Rosenthal. 2002. Different Modes of Hypertrophy in Skeletal Muscle Fibers. The Journal of Cell Biology. 156(4): 751-760.

Huber, M. D. and L. Gerace. 2007. The Size-wise Nucleus: Nuclear Volume Control in Eukaryotes. J Cell Biol. 179(4): 583-4.

Evangelista, F. S., P. C. Brum, and J. E. Krieger. 2003. Duration-controlled Swimming Exercise Training Induces Cardiac Hypertrophy in Mice. Braz J Med Biol Res. 36(12): 1751-9.

Grounds, M. D., et al. 2002. The Role of Stem Cells in Skeletal and Cardiac Muscle Repair. Journal of Histochemistry & Cytochemistry. 50(5): 589-610.

King, D. W. and P. D. Gollnick. 1970. Ultrastructure of Rat Heart and Liver After Exhaustive Exercise. Am J Physiol. 218(4): 1150-5.

Friden, J. and R. L. Lieber. 1992. Structural and Mechanical Basis of Exercise-induced Muscle Injury. Med Sci Sports Exerc. 24(5): 521-30.

Le Grand, F. and M. A. Rudnicki. 2007. Skeletal Muscle Satellite Cells and Adult Myogenesis. Curr Opin Cell Biol. 19(6): 628-33.

Jansen, S., et al. 2000. Aluminium Accumulation in Leaves of Rubiaceae: Systematic and Phylogenetic Implications. Annals of Botany. 85(1): 91-101.

Ibrahim, M. B., et al. 2016. Sub-acute and Chronic Toxicity Profiles Of Markhamia Tomentosa Ethanolic Leaf Extract In Rats. J Ethnopharmacol. 193: 68-75.

Mohamed, E. A., et al. 2011. Toxicity Evaluation of a Standardised 50% Ethanol Extract of Orthosiphon Stamineus. J Ethnopharmacol. 133(2): 358-63.






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