Biodegration of Dye Using Free Laccase and Sol-gel Laccase

Abstract

Malachite green oxalate dyes are of synthetic origin and their environmental existence is not well understood. They are resistant to direct aerobic bacterial degradation and form potentially carcinogenic aromatic amines. This study shows that applying the oxidative processes of enzymatic treatment with free laccase and sol-gel laccase could lead to dye degradation. The degradation of dye malachite green oxalate using free laccase and sol-gel laccase, respectively were 37% and 13% at 1 hour reaction. The optimum pH for the free laccase and sol-gel laccase respectively were at pH 5 and pH 6. The optimal temperature for degradation of dye by sol-gel laccase was at 40°C. These results showed that free laccase and sol-gel laccase have good performance in the degradation of malachite green oxalate dyes. Pewarna oxalate hijau Malachite adalah sintetik asli dan kewujudan semulajadinya tidak begitu diketahui. Ia bersifat kalis degradasi bakteria aerobik dan membentuk amina aromatik yang berpotensi karsinogen. Kajian ini menunjukkan bahawa penggunaan proses oksidatif untuk rawatan enzim dengan lakase bebas dan lakase sol-gel boleh membawa kepada degradasi pewarna. Degradasi pewarna malachite hijau oxalate yang menggunakan lakase bebas dan lakase sol-gel, masing-masing adalah sebanyak 37% dan 13% pada 1 jam reaksi. pH optimum untuk lakase bebas and lakase sol gel, masing-masing adalah pada pH 5 dan pH 6. Suhu optimum bagi degradasi pewarna oleh lakase sol-gel adalah pada 40°C.