IN VITRO PRESERVATION OF RODENT TUBER (TYPHONIUM FLAGELLIFORME LODD.) PEKALONGAN ACCESSION WITH PACLOBUTRAZOL
DOI:
https://doi.org/10.11113/jt.v81.12818Keywords:
Typhonium flagelliforme (Lodd.), in vitro, paclobutrazol, normal shoot regenerationAbstract
Rodent tuber plant (Typhonium flagelliforme Lodd.), as one of the most potent medicinal plants, has to be developed as an active ingredient of degenerative drugs, including cancer drugs. However, this enormous potential must be supported by sustainable cultivation of the plant. The conventional preservation of rodent tuber can be done by planting various accessions in the field, but it will need land availability and intensive plant maintenance. Preservation through in vitro culture is an alternative method that can be used. The aim of this study was to determine the effect of paclobutrazol in suppressing the growth of rodent tuber cultures, and test the ability of culture regeneration after being preserved with paclobutrazol. The media formulation used was Murashige and Skoog (1962) (MS)Â with the addition of paclobutrazol at concentrations of 4, 5, 6, and 7 mg L-1. The results showed that using paclobutrazol at 5 mg L-1 was the best concentration that can inhibit the elongation of buds, seedling formation, leaf formation, and root elongation until 5 months. Cultures of paclobutrazol treatment at 5 mg L-1 had shoot heights, number of shoots, number of leaves, and root lengths of 0.49, 2.33, 7.23, and 0.3 cm, respectively. Paclobutrazol could inhibit the growth of in vitroculture of rodent tuber and prolong the shelf life of culture up to 5 months. The culture of rodent tuber from paclobutrazol treatment had normal growth and regenerative ability after transfer to the medium regeneration.
References
Cathey, H. M. 1975. Comparative Plant Growth Retarding Activities of Ancymidol with ACPC, Phosfon, Chlormequat, and SADH on Ornamental Plant Species. Horticultural Sciences. 10(3): 204-215.
Chan, L. K., Koh, W. Y., and Tengku-Muhammad, T. S. 2005. Comparison of Cytotoxic Activities between In Vitro and Field Grown Plants of Typhonium flagelliforme (Lodd.) Blume. Journal of Plant Biology. 48(1): 25-31.
Department of Agricultural Resources. 2012. Active Ingredient Paclobutrazol: Review Conducted by MDAR and MassDEP for Use in Sensitive Areas of Rights of Way in Massachusetts. Executive office of Energy and Environmental Affairs: Massachusetts.
Essai. 1986. Medicinal Herbs Index in Indonesia. PT Essai Indonesia, Jakarta.
George, E. F., and Sherington, P. D. 1984. Plant Propagation by Tissue Culture. England: Eastern Press.
Graebe, J. E. 1987. Gibberellin Biosynthesis and Control. Annual Review in Plant Physiology. 38: 419-465.
Hassan, M. L., Behrooz, E., and Esmaiel, C. 2011. Hinokitiol and Activated Charcoal Influence the Microtuberization and Growth of Potato (Solanum tuberasum cv. Agria) Plantlets in vitro. Australian Journal of Crop Science. 5(11): 1481-1485.
Huang, P., Karagianis, G., and Waterman, P. G. 2004. Chemical Constituents from Typhonium flagelliforme. Journal of Chinese Medicine Materials. 27(3): 173-175.
Kucharska, D., and Orlikowska, T. 2008. The Influence of Paclobutrazol in the Rooting Medium on the Quality of Chrysanthemum Vitroplants. Journal of Fruit and Ornamental Plant Research. 16: 417-424.
Lai, C. S., Mas, R. H., Nair, N. K., Majid, M. I., Mansor, S. M., and Navaratnam, V. 2008. Typhonium flagelliforme Inhibits Cancer Cell Growth In Vitro and Induces Apoptosis: An Evaluation by the Bioactivity Guided Approach. Journal of Ethnopharmacology. 118(1): 14-20.
Lai, C. S., Mas, R. H., Nair, N.K., Mansor, S. M., and Navaratnam, V. 2010. Chemical Constituents and In Vitro Anticancer Activity of Typhonium flagelliforme (Araceae). Journal of Ethnopharmacology. 127(2): 486-494.
Lestari, E. G., and Purnamaningsih, R. 2005. Konservasi In Vitro Tanaman Obat Daun Dewa Melalui Pertumbuhan Minimal. Jurnal AgroBiogen. 1(2): 68-72.
Mankaran, S., Dinesh, K., Deepak, S., and Gurmeet, S. 2013. Typhonium flagelliforme: A Multipurpose Plant. International Research Journal of Pharmacy. 4(3): 45-48.
Hamama, L., Voisine, L., Naouar, A., Gala, R., Cesbron, D., Michel, G., Leplat, F., Foucher, F., Oyant, H.S., and Dorion, N. 2012. Effect of GA3 and Paclobutrazol on Adventitious Shoot regeneration of Two Pelargonium sp. Proceeding 7th IS on In vitro Culture and Horticultural Breeding. 187-194.
Meijon, M., Canal, M. J., Valledor, L., Rodriguez, R., and Feito, I. 2011. Epigenetic and Physiological Effects of Gibberellin Inhibitors and Chemical Pruners on the Floral Transition of Azalea. Physiologia Plantarum.141: 276-288.
Mitoi, E. M., Holobiuc, I., and Blindu, R. 2009. The Effect of Mannitol on Antioxidative Enzymes In Vitro Long Term Cultures of Dianthus tenuifolius and Dianthus spiculifolius. Romanian Journal of Biology Plant Biology. 54(1): 25-33.
Mohan, S., Abdul, A. B., Abdelwahab, S. I., Al-Zubairi, A. S., Aspollah, S. M., Abdullah, R., Tahan, M. M., Beng, N. K., and Isa, N. M. 2010. Typhonium flagelliforme Inhibits the Proliferation of Murine Leukemia WEHI-3 Cells In Vitro and Induces Apoptosis In Vivo. Leukemia Research. 34(11): 1483-1492.
Nobakht, G. M., Kadir, M. A., and Stanslas, J. 2010. Analysis of Preliminary Phytochemical Screening of Typhonium flagelliforme. African Journal of Biotechnology. 9(11): 1655-1657.
Purnamaningsih, R., Mariska, I., and Supriati, Y. 2009. Penggunaan Paclobutrazol da ABA dalam Perbanyakan Nenas Simadu Melalui Kultur In Vitro. Berita Biologi. 9: 6.
Purnamaningsih, R., and Sianipar, N. F. 2018. Analysis of Bioactive Compounds and Morphological Traits in Indonesian Rodent Tuber Mutant Clones of Pekalongan Accession Using GC-MS. Jurnal Teknologi. 80(2): 131-136.
Roostika, I., Purnamaningsih, R., and Noviati, A. V. 2008. Pengaruh Sumber Karbon dan Kondisi Inkubasi Terhadap Pertumbuhan In Vitro Purwoceng (Pimpinella pruatjan Molk.). Jurnal AgroBiogen. 4(2): 65-69.
Roostika, I., Purnamaningsih, R., and Darwati, I. 2009. Penyimpanan In Vitro Tanaman Purwoceng (Pimpinella pruatjan Molk.) Melalui Aplikasi Pengenceran Media dan Paclobutrazol. Jurnal Littri. 15(2): 84-90.
Sianipar, N. F., Rustikawati, Maarisit, W., Wantho, A., and Sidabutar, D. N. R. 2011. Embryonic Calli Induction, Proliferation and Regeneration of Rodent Tuber Plant (Typhonium flagelliforme Lodd.) by Single Node Culture. International Conference on Biological Science, Faculty of Biology,Universitas Gadjah Mada. 84-92.
Sianipar, N. F., Maarisit, W., and Valencia, A. 2013. Toxic Activity of Hexane Extract and Its Fraction Column Chromatography of Rodent Tuber Plant (Typhonium flagelliforme Lodd.) on Artemia salina. Hayati Journal of Biosciences. 14(1): 1-7.
Somasundaram, R., Jaleel, C. A., Abraham, S. S., Azooz, M. M., and Panneerselvam, R. 2009. Role of Paclobutrazol and ABA in Drought Stress Amelioration in Sesamum indicum L. Global Journal of Molecular Sciences. 4(2): 56-62.
Subotic, A., Jevremovic, S., Trifunovic, M., Petric, M., Milosevic, S., and Grubisic, D. 2009. The Influence of Gibberellic Acid and Paclobutrazol on Induction of Somatic Embryogenesis in Wild Type and Hairy Root Cultures of Centaurium erythraea Gillib. African Journal of Biotechnology. 8(14): 3223-3228.
Syahid, S. F. 2007. Pengaruh Retardan Paclobutrazol Terhadap Pertumbuhan Temu Lawak (Curcuma xanthorrhiza) Selama Konservasi In Vitro. Jurnal Littri. 13(3): 93-97.
Syahid, S. F., and Kristina, N. N. 2007. Induksi dan Regenerasi Kalus keladi tikus (Typonium flagelliforme Lodd) Secara In Vitro. Jurnal Littri. 13(4): 142-146.
Surachman, D. 2009. Penggunaan Beberapa Taraf Konservasi Paclobutrazol dalam Konservasi Keladi Tikus (Typonium flagelliforme Lodd) In Vitro. Buletin Teknik Pertanian. 14(1): 31-33.
Te-chato, S., Nujeen, P., and Muangsorn, S. 2009. Paclobutrazol Enhance Budbreak and Flowering of Friederick’s Dendrobium Orchid In Vitro. Journal of Agricultural Technology. 5(1): 157-165.
Widyastuti, N. 2000. Pelestarian Tanaman Pangan dengan Teknik Kultur In Vitro. Jurnal Teknologi Lingkungan. 1(3): 206-211.
Downloads
Published
Issue
Section
License
Copyright of articles that appear in Jurnal Teknologi belongs exclusively to Penerbit Universiti Teknologi Malaysia (Penerbit UTM Press). This copyright covers the rights to reproduce the article, including reprints, electronic reproductions, or any other reproductions of similar nature.
