VOLATOLOMICS COMBINED TERAHERTZ TIME - DOMAIN SPECTRAL ANALYSES OF COLON CANCER IN VITRO

Authors

  • Ahmad Zulhilmi Arshad Department of Physics, Faculty of Science, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia
  • Yusof Munajat Department of Physics, Faculty of Science, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia
  • Sib Krishna Ghoshal Department of Physics, Faculty of Science, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia
  • Rahman Jamal UKM Medical Molecular Biology Institute, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Batu 9 Cheras, Wilayah Persekutuan Kuala Lumpur, Malaysia
  • Nor Adzimah Johdi UKM Medical Molecular Biology Institute, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Batu 9 Cheras, Wilayah Persekutuan Kuala Lumpur, Malaysia
  • Raja Kamarulzaman Raja Ibrahim
  • Hanif Zulkhairi UKM Medical Molecular Biology Institute, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Batu 9 Cheras, Wilayah Persekutuan Kuala Lumpur, Malaysia

DOI:

https://doi.org/10.11113/jt.v81.13310

Keywords:

Volatolomics analysis, colon cancer, Chromatography, Terahertz spectra, gas analysis

Abstract

Terahertz time-domain spectroscopy (THz-TDS) offers a great advantage for the analysis of biological samples. In this study, we combined THz-TDS with volatolomics analysis and analyzed 2 colon cell lines via in vitro settings. The release of volatile organic compounds (VOCs) was measured from the normal colon (CCD112CoN) and cancer colon (COLO320DM) cell lines which were grown in sealed flasks. Data validation were carried out with principal component analysis (PCA) and partial least square (PLS) scores while the chemometric analyses were performed using Camo Unscrambler X software. In-depth THz-TDS spectral analysis of the cancer colon (COLO320DM) cell line shows significant traces of benzamide gas when validated using gas chromatography-mass selective detection (GC-MSD) system. This preliminary data shows the potential use of identification and quantification of benzamide compound in the cancer colon cells and this could provide useful insight towards cancer drug design and therapy.

Author Biography

  • Yusof Munajat, Department of Physics, Faculty of Science, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia
    Physics Department

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Published

2019-08-19

Issue

Section

Science and Engineering

How to Cite

VOLATOLOMICS COMBINED TERAHERTZ TIME - DOMAIN SPECTRAL ANALYSES OF COLON CANCER IN VITRO. (2019). Jurnal Teknologi (Sciences & Engineering), 81(5). https://doi.org/10.11113/jt.v81.13310