ISOLATION AND IDENTIFICATION OF MICROALGAE FROM HIGH NITRATE LANDFILL LEACHATE
DOI:
https://doi.org/10.11113/jt.v81.13581Keywords:
Microalgae, Chlorella vulgaris, Tetradesmus obliquus, Microalgae BiomassAbstract
This study aims to isolate and identify microalgae capable of growing in high nitrate (N-NO3-) landfill leachate. Source of isolation was collected from a landfill leachate treatment plant and identified according to morphological characteristics and analysis of partial 18S and 28S rRNA genes. The isolates, identified as Chlorella vulgaris UPSI-JRM01 and Tetradesmus obliquus UPSI-JRM02 were capable of growing at high N-NO3- concentration of nitrified landfill leachate, which is up to 1500 mg/L. The biomass productivities of Chlorella vulgaris and Tetradesmus obliquus were 36.28 mg/L/day and 40.49 mg/L/day, with 44% and 37% N-NO3- removal, respectively. The biomass of Chlorella vulgaris and Tetradesmus obliquus consisted of 17.72% and 16.32% lipid and, 21.93% and 25.43% carbohydrate, respectively. The protein contents (>50%) were higher than lipid and carbohydrate contents for both microalgal species. The newly isolated microalgae species will be useful for future applications of high NO3- wastewater treatment and microalgae biomass production.
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