PEPTIDE EXTRACTS FROM TEMPEH PROTECT AGAINST PHOTOAGING VIA DOWN-REGULATING MATRIX METALLOPROTEINASE-3 GENE EXPRESSION

Authors

  • Steffanus Pranoto Hallis Graduate School of Biotechnology, Atma Jaya Catholic University of Indonesia, 12930, Jakarta, Indonesia
  • Yanti Yanti Department of Food Technology, Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, 15345, Tangerang, Indonesia
  • Maggy Thenawidjaja Suhartono Department of Food Science and Technology, Faculty of Agricultural Technology, Bogor Agricultural University, 16680, Bogor, Indonesia
  • Soegianto Ali dFaculty of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, 14440, Jakarta, Indonesia

DOI:

https://doi.org/10.11113/jt.v82.13961

Keywords:

Tempeh, peptide extracts, anti-photoaging activity, UVB-induced mice, MMP-3

Abstract

Photoaging damages skin tissue via activation of matrix metalloproteinases (MMPs) and MMP-3 induces activation of MMP-1 and 9. Tempeh is made from fermented soybean in Indonesia and has been known for its high protein content. This study was aimed to investigate the effectiveness of peptide extracts from tempeh against photoaging at gene level in UVB-treated mice. Peptide was extracted from soybean and black soybean tempeh, and identified for its amino acid profiling. Photoaging model was made by gradient UVB exposure to seven groups of mice for 4 weeks. Mice were supplemented with 100 and 300 mg/kg body weight dosages of peptide extracts from soybean and black soybean tempeh. Dorsal skin tissue was collected for histology staining and quantitative polymerase chain reaction (qPCR). This present study found that both tempeh peptide extracts were rich in glutamic acid and branched-chain amino acids. Histology staining showed that tempeh protected mice skin from photoaging. At 100 and 300 mg/kg, both tempeh peptide extracts significantly attenuated MMP-3gene expression that lead to the decrease level of MMP-1 and MMP-9 gene expression. These results suggest that supplementation of both tempeh peptide extracts may possess anti-photoaging effect via altering MMP-3 gene expression in UVB-treated mice.

 

Author Biography

  • Yanti Yanti, Department of Food Technology, Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, 15345, Tangerang, Indonesia
    Department of Food Technology, Faculty of Biotechnology

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Published

2019-12-04

Issue

Vol. 82 No. 1: January 2020

Section

Science and Engineering

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How to Cite

PEPTIDE EXTRACTS FROM TEMPEH PROTECT AGAINST PHOTOAGING VIA DOWN-REGULATING MATRIX METALLOPROTEINASE-3 GENE EXPRESSION. (2019). Jurnal Teknologi (Sciences & Engineering), 82(1). https://doi.org/10.11113/jt.v82.13961