NOVEL COMPOSITE OF SILVER-BACTERIAL CELLULOSE (Ag-BC) FROM SIWALAN SAP (Borassus flabellifer) AND ITS ANTIBACTERIAL ACTIVITY
DOI:
https://doi.org/10.11113/jurnalteknologi.v83.14363Keywords:
Bacterial cellulose, silver, composite, antibacterial activity, Borassus flabelliferAbstract
Recently, a wound healing from natural composite with excellent properties is in a high demand. In this study, a novel composite of bacterial cellulose made from Siwalan sap (Borassus flabellifer) was achieved. Siwalan is a common plant in Java Island of Indonesia and the application is very limited for beverage only. This study aims to determine the effect of the AgNO3/NaBH4 concentration ratio in the development of Ag-BC composites and its antibacterial properties from Siwalan sap. Ag-BC composites were prepared by impregnating the silver solution into the BC matrix through the reduction process with NaBH4. Characterization of Ag-BC composites conducted using Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and Energy Dispersive X-ray (EDX). Antibacterial assay was performed using disc diffusion method against Salmonella typhimurium (Gram-negative) and Staphylococcus aureus (Gram-positive) bacteria. The characterization of Ag-BC composite shows the nanostructure of BC with a length of fiber around 35-60 nm in width. The SEM-EDX micrograph showed that silver particles were impregnated into the BC matrix. Antibacterial activity test results showed that the Ag-BC composite had the ability to inhibit the bacteria S. typhimurium and S. aureus with good inhibition. This result showed the potential application of Ag-BC composite from Siwalan plant as a natural material for medical and pharmaceutical purpose, especially as an antibacterial agent.
References
Almaida I F, Pereira T, Silva N H C S, Gomes F P, Silvestre A J D, Freire C S R, Sousa Lobo J M, and Costa P C 2014 Bacterial cellulose membranes as drug delivery systems: An in vivo skin compatibility study Eur. J. Pharm. Biopharm. 86. 332–336
Brown, R.M., Willson. J.H., Richardson, C.L.,1976. Cellulose biosynthesis in Acetobacter xylinum: Visualization of the site of synthesis and direct measurement of the in vivo proses. Proceedings of the National Academy of sciences of the United States of America. 73(12): 4565-4569.
Chaloupka K, Malam Y & Seifalian AM . 2010. Nanosilver as a new generation of nanoproduct in biomedical applications. Journal Trends Biotechnol , 28: 580–588.
Halib, N., Amin, M.C.I.M., dan Ahmad, I., 2012. Physicochemical properties and characterization of Nata de coco from local food industries a source of cellulose. Sains Malaysiana, 41(2): 205-211
He, J., Kunitake, T., dan Nakao, A. (2003). Facile in situ synthesis of noble metal nanoparticles in porous cellulose fibers. Chemistry of Materials, 15: 4401-4406.
Indrianingsih, W., Rosyida, V.T., Jatmiko, T.H., Prasetyo, D.J., Poeloengsih, C.D., Apriyana, W., Nisa, K., Nurhayati, S., Hermawan., Darsih, C., Pratiwi, D., Suwanto, A., Ratih, D., 2017. Premilinary Study On Biosynthesis And Characterization Of Bacteria Cellulose Films From Coconut Water. IOP Conf. Series: Earth and Enviromental Science., doi:10.1088/1755-1315/101/1/012010
Indrianingsih, A.W., Maryana, R., Rosyida, V.T., Apriyana, W., Jatmiko, T.H., Hayati, S.N., 2017. Antibacterial Activity Of Silver-Modified Bacterial Cellulose Produced From Coconut Water for Wound Dressing Application. .Chemistry Research Journal, 2(6): 251-261
Kurosumi,A., Sasaki, C., Yamashita, Nakamura, Y., 2009. Utilization of various fruit juice as carbon source for production of bacterial cellulose by Acetobacter xylinum NBRC 13693. Carbohydrate Polymer. 76 (2): 333-335
Maneerung, T., Tokura, S., Rujiravanit, R., 2007. Impregnation of silver nanoparticles into bacterial cellulose for antimicrobial wound dressing. Carbohydrate Polymers (72): 43-51
Mardiyah,S., 2017. Pengaruh Lama Pemanasan Terhadap Kadar Alkohol Pada Nira Siwalan (Borassus flabellifer). The journal Of Muhamadiyah Medical Laboratory Technologist, 2(1): 99-15
Maulana, M.I., Syahbanu, I., Harlia., 2017. Sintesis dan karakterisasi material konduktif film komposit polipirol (Ppy)/selulosa bakteri. JKK, 6(3): 11-18
Mohite, Bhavana & Patil, Satis V. 2016. In situ development of nanosilver-impregnated bacterial cellulose for sustainable released antimicrobial wound dressing. Biomater Funct Mater, 14( 1): 53 – 58
Pal,S., Nisi, R., Stoppa, M., Licciulli, A., 2017. Silver-Functionalized Bacterial Cellulose as Antibacterial Membrane for wound-Healing Applications. American Journal Chemical Sociaty, (2): 3632-3639
Prasad, S.B. 2013. Current Understanding of Synthesis and Pharmacological Aspects of Silver Nanoparticles. American Journal of Phytomedicine and Clinical Therapeutics, 1(7): 536-547.
Salasa, D., Aritonang, H., Kamu, V.S., 2016. Sintesis Nanopartikel Perak (Ag) dengan Reduktor Natrium Borohidrat (NaBH4) Menggunakan Matriks Nata de coco. Chem. Prog, 9(2): 40-47
Shidlovskiy, I.P., Shumilova, A.A., dan Shishatskaya, E.I., 2017. Preparation and caracterization of Bacterial Cellulose Composites with Silver Nanoparticles. Journal of Siberian Federal University, Biologi, 10(2): 255-264
Volova, T.G., Shumilova, A.A., Shidlovskiy, I.P., Nikolaeva, E.D., 2018. Antibacterial properties of film of cellulose composites with silver nanoparticles and antibiotics. Polymer Testingi, 65: 54-68
Wu, J., Zheng, Y., Song, W., Luan, J., Wen, X., Wu, Z., Chen, X., Wang, Q., Guo, S., 2013. In situ synthesis of silver-nanoparticles/bacterial cellulose composites for slow-released antimicrobial wound dressing. Carbohydrate Polymers.
Yang W, Shen C, Ji Q, An H, Wang J, Liu Q & Zhang Z. 2009. Food storage material silver nanoparticles interfere with DNA replication fidelity and bind with DNA. Nanotechnology (20): 0957–4484.
Yunita, M., Hendrawan, Y., Yulianingsih, R., 2015. Analisis Kuantitatif Mikrobiologi Pada Makanan Penerbangan (Aerofood ACS) Garuda Indonesia Berdasarkan TPC (Total Plate Count) dengan Metode Pour Plate. Jurnal Keteknikan Pertanian Tropis dan Biosistem,3(3): 237-248. DOI : http://dx.doi.org/10.11113/jt.v79.9987
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