Potential Use of Liquid Pinapple Waste for Bioethanol Production by Immobilized Bakers’ Yeast

Authors

  • Nor Azimah Mohd Zain Faculty of Biosciences and Bioengineering, Universiti Teknologi Malaysia, 81310, UTM Johor Bahru, Johor Darul Ta’azim, Malaysia
  • Ng Sew Shen Faculty of Biosciences and Bioengineering, Universiti Teknologi Malaysia, 81310, UTM Johor Bahru, Johor Darul Ta’azim, Malaysia
  • Mohd Suardi Suhaimi Faculty of Chemical Engineering, Universiti Teknologi Malaysia, 81310, UTM Johor Bahru, Johor Darul Ta’azim, Malaysia
  • Nor Badzilah Hasan Faculty of Biosciences and Bioengineering, Universiti Teknologi Malaysia, 81310, UTM Johor Bahru, Johor Darul Ta’azim, Malaysia
  • Siti Nurbalqis Aziman Faculty of Biosciences and Bioengineering, Universiti Teknologi Malaysia, 81310, UTM Johor Bahru, Johor Darul Ta’azim, Malaysia

DOI:

https://doi.org/10.11113/jt.v59.1582

Keywords:

Baker’s yeast, immobilization, PVA–alginate beads, bioethanol production, liquid pineapple waste

Abstract

Bioethanol is the most extensively used biofuel for transportation in the world. Nowadays, researchers are focusing in producing bioethanol from crops and agricultural wastes. Malaysia contributes to 1% of world pineapple production which corresponds to an enormous amount of waste generation per year. Utilization of waste from pineapple processing industry for bioethanol production could help to reduce potential environmental issues. In this study, Bakers’ yeast (Saccharomyces cerevisiae) was immobilized in modified PVA–alginate beads to produce bioethanol from liquid pineapple waste. The results revealed that by using 10 % (w/v) of the immobilized cells highest overall productivity of 0.0752 g/L/h and maximum production of 5.4179 g/L of bioethanol could be achieved. Although its maximum productivity of immobilized yeast was 0.0752 g/L/h which appreciably lower than that of the free cells, this deficiency is balanced by its overall productivity which is almost 50 % higher compared to that of free cells.

References

Ibeto, C. N., Ofoefule, A. U. & Agbo, K. E.. 2011. A. Global. Overview

of Biomass Potentials for Bioethanol Production: A Renewable

Alternative Fuel. Trends Appl Sci Res. 6: 410–425.

Ban-Koffi, L., Han, Y. W. 1990. Alcohol Production from Pineapple

Waste. World J Microb Biot. 6: 281–284.

Tanaka, K., Hilary, Z. D., Ishizaki, A. 1999. Investigation of the Utility

of Pineapple Juice and Pineapple Waste Material as Low-cost Substrate

for Ethanol Fermentation by Zymomonas Mobilis. J. Biosci. Bioeng. 87:

–646.

Cardona, C. A., Sánchez, Ó. J. & Gutiérrez, L. F. 2009. Microorganisms

for Ethanol Production. Process Synthesis for Fuel Ethanol Production.

CRC Press.

Claassen, P. A. M., Van Lier,J. B., Lopez Contreras, A. M., Van Niel, E.

W. J., Sijtsma, L., Stams, A. J. M.. 1999. et al. Utilisation of Biomass for

the Supply of Energy Carriers. Appl. Microbiol. Biotechnol. 52: 741–755.

Nigam, J. N. 2000. Continuous Ethanol Production from Pineapple

Cannery Waste Using Immobilized Yeast Cells. J Biotechnol. 80: 189–

Miller, G. 1959. Use of DNS Reagent for Determination of Reducing

Sugars. Anal Chem . 31: 426–428.

Idris, A. and Suzana, W. 2006. Effect of Sodium Alginate Concentration,

Bead Diameter, Initial Ph and Temperature on Lactic Acid Production

From Pineapple Waste Using Immobilized Lactobacillus Delbrueckii.

Process Biochem. 41: 1117–1123.

Zain, N. A. M.2009. Modification of PVA- Alginate Immobilization

Matrix to Immobilize Invertase. Doctor of Philosophy. Universiti

Teknologi Malaysia.

Liu, R., Shen, F. 2008. Impacts of Main Factors on Bioethanol

Fermentation From Stalk Juice of Sweet Sorghum by Immobilized

Saccharomyces Cerevisiae (CICC 1308). Bioresour. Technol. 99: 847–

Drapcho, C. M., Nhuan, N. P., Walker, T. H. 2008. Biofuels Engineering

Process Technology. United States of America. McGraw-Hill

Companies.

Olga, D. S., James, C. D. P., Jacobus, A. 2008. The Alcohol

Dehydrogenases of Saccharomyces Cerevisiae: A Comprehensive

Review. FEMS 8: 967–978.

Knezevic, Z., Bobic, S., Milutinovic, A. 2002. Obradovic B, Mojovic L,

Bugarski B. Alginate-Immobilized Lipase by Electrostatic Extrusion for

the Purpose o Palm Oil Hydrolysis in Lecithin/Isooctane System. Process

Biochem. 38: 313–318.

Lee, K. H., Choi, I. S., Kimb, Y. G., Yang, D. J., Bae, H. J. 2011.

Enhanced Production of Bioethanol and Ultrastructural Characteristics of

Reused Saccharomyces Cerevisiae Immobilized Calcium Alginate Beads.

Bioresource Technol. 102: 8191–8198.

Kourkoutas, Y., Kanellaki, M., Koutinas, A., Tzia, C. 2005. Effect of

Fermentation Conditions and Immobilization Supports on the Wine

Making. J Food Process Eng. 69: 115–123.

Downloads

Published

2012-09-15

How to Cite

Potential Use of Liquid Pinapple Waste for Bioethanol Production by Immobilized Bakers’ Yeast. (2012). Jurnal Teknologi, 59(1). https://doi.org/10.11113/jt.v59.1582