ANTI-INFLAMMATORY AND ORAL TOXICITY ASSESSMENT OF R-38TM: A TRADITIONAL HERBAL SUPPLEMENT FOR ARTHRITIS

Authors

  • Anis Amirah Mohamad Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu Darul Iman, Malaysia https://orcid.org/0000-0002-2178-3580
  • Fadzilah Adibah Abdul Majid Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu Darul Iman, Malaysia
  • Suvik Assaw School of Fisheries and Food Science, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu Darul Iman, Malaysia
  • Fadhilah Zainal Abidin Kulliyyah of Dentistry, International Islamic University Malaysia, Indera Mahkota, 25200 Kuantan, Pahang, Malaysia https://orcid.org/0000-0002-5848-3581
  • Anis Fadhlina Institute of Food Security and Sustainable Agriculture, Universiti Malaysia Kelantan, 17600 Jeli, Kelantan, Malaysia
  • Sudheer Kumar Aluru Division of Animal Biotechnology, Department of Zoology, Sri Venkateswara University, Tirupati-517506, Andhra Pradesh, India https://orcid.org/0000-0002-4619-2488
  • Siti Nurazwa Zainol Proliv Life Sciences Sdn Bhd, D-1-15, Residensi Bistaria, Jln Ulu Kelang, Taman Ukay Bistari, 68000 Ampang, Selangor Darul Ehsan, Malaysia.
  • Najat Jabbar Ahmed Erbil Technical Health College, Erbil Polytechnic University, Erbil, Iraq

DOI:

https://doi.org/10.11113/jurnalteknologi.v84.16919

Keywords:

Botanical medicine, R-38TM, anti-inflammatory, oral toxicity, rosmarinic acid, arthritis

Abstract

R-38TM is a traditional herbal supplement for treating arthritis-related conditions. High-performance liquid chromatography (HPLC) analysis was performed for identification and quantification of rosmarinic acid in the R-38TM water extract. The anti-arthritic potential of the R-38TM water extract was investigated by measuring the production of IL-6 and TNF-α in inflamed cells. Xanthine oxidase (XO) and cyclooxygenase-2 (COX-2) inhibition assays were also conducted. The cytotoxic effect of R-38TM water extract was investigated on HSF1184 cell line. Acute and subacute oral toxicity studies were conducted on female Sprague-Dawley (SD) rats. The rosmarinic acid was identified at 1.208 min (3.61 %w/w). The inflamed cells showed a decrease in the production of IL-6 (55.9%) and TNF-α (52.13%). The COX-2 and XO enzymes were moderately inhibited by R-38TM water extract. The cytotoxicity analysis showed no cytotoxic effect on the cell. The acute and subacute oral toxicity studies revealed no mortality and normal body weight at all doses. There were no significant differences (p > 0.05) in organ weight, hematological and biochemical parameters, and histology of liver and kidneys with the control group. In conclusion, R-38TM water extract exhibited no toxic effect orally and may possess potential therapeutic properties against pro-inflammatory diseases including arthritis.

Author Biography

Fadzilah Adibah Abdul Majid, Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu Darul Iman, Malaysia

   

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Published

2021-12-14

How to Cite

Mohamad, A. A. ., Abdul Majid, F. A., Assaw, S. ., Zainal Abidin, F. ., Fadhlina, A. ., Kumar Aluru, S., Zainol, S. N. ., & Ahmed, N. J. . (2021). ANTI-INFLAMMATORY AND ORAL TOXICITY ASSESSMENT OF R-38TM: A TRADITIONAL HERBAL SUPPLEMENT FOR ARTHRITIS. Jurnal Teknologi, 84(1), 249-262. https://doi.org/10.11113/jurnalteknologi.v84.16919

Issue

Section

Science and Engineering