IN VITRO CYTOTOXIC EVALUATION AND APOPTOTIC EFFECTS OF DILLAPIOLE ON HUMAN NASAL SQUAMOUS CELL CARCINOMA

Authors

  • Nur Batrisyia Ruslan Centre of Preclinical Sciences Studies, Faculty of Dentistry, Universiti Teknologi MARA (UiTM), Sungai Buloh, Selangor, Malaysia
  • Indah Mohd Amin Centre of Preclinical Sciences Studies, Faculty of Dentistry, Universiti Teknologi MARA (UiTM), Sungai Buloh, Selangor, Malaysia https://orcid.org/0000-0001-6964-9017
  • Narimah Abdul Hamid Hasani Department of Biochemistry and Molecular Medicine, Faculty of Medicine, Universiti Teknologi MARA (UiTM), Sungai Buloh, Selangor, Malaysia https://orcid.org/0000-0002-0056-3909
  • Vivi Noryati Ahmad Centre of Preclinical Sciences Studies, Faculty of Dentistry, Universiti Teknologi MARA (UiTM), Sungai Buloh, Selangor, Malaysia
  • Farizan Aris School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM) Shah Alam, Selangor, Malaysia https://orcid.org/0000-0002-7973-4845
  • Goot Heah Khor Centre of Preclinical Sciences Studies, Faculty of Dentistry, Universiti Teknologi MARA (UiTM), Sungai Buloh, Selangor, Malaysia https://orcid.org/0000-0002-4686-7668

DOI:

https://doi.org/10.11113/jurnalteknologi.v83.17077

Keywords:

RPMI 2650 cells, dillapiole, cytotoxicity, apoptosis, Caspase 8 and BCL2 proteins

Abstract

Dillapiole is one of the major compounds in Peperomia pellucida. Dillapiole exerts cytotoxicity effect on several cancer cells such as breast and colon with a minimal effect on normal cells. However, its effect on cancers in head and neck region remains ambiguous. Therefore, in the current study, the cytotoxicity effect of dillapiole on human nasal squamous cell carcinoma, RPMI 2650 and the underlying mechanism were investigated. Normal human gingival fibroblast, HGnF cells was used as comparison. Cisplatin and untreated cells were used as positive and negative controls, respectively. Cell cytotoxicity was determined using WST-1 assay; and validated by trypan blue exclusion assays. Cell death mechanism was determined using Annexin V-FITC detection kit and analyzed by flow cytometer. The cytotoxic effect of dillapiole on RPMI 2650 cells was shown significantly increased with the increasing amount of dillapiole from 25.93 ± 6.39 % to 83.87 ± 8.43 % (p<0.05, n=3) with the respective IC50 and IC75 of 46 µM and 125 µM. None of both IC50 and IC75 were obtained for HGnF cells up to 150 µM. Loss of normal shape, cytoplasm shrinkage and reduction in cell volume were detected in RPMI 2650 cells after dillapiole treatment at respective IC50 (46 µM) and IC75 (125 µM), indicating apoptosis. These findings indicate that dillapiole cytotoxicity effect is more selective towards RPMI 2650 compared to HGnF cells and the mechanism of death was through the induction of apoptosis.

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Published

2021-08-08

How to Cite

Ruslan, N. B., Mohd Amin, I., Abdul Hamid Hasani, N., Ahmad, V. N. ., Aris, F. ., & Khor, G. H. (2021). IN VITRO CYTOTOXIC EVALUATION AND APOPTOTIC EFFECTS OF DILLAPIOLE ON HUMAN NASAL SQUAMOUS CELL CARCINOMA. Jurnal Teknologi, 83(5), 93-99. https://doi.org/10.11113/jurnalteknologi.v83.17077

Issue

Section

Science and Engineering