CHARACTERIZATION OF THERMOSTABLE BETA-1,4-GALACTANASE AND ITS APPLICATION IN HYDROLYSIS OF PECTIN FROM SWEET POTATO (Ipomoea batatas (L.) Lam) PEELS

Authors

  • Noor Faizah Ismail ᵃSchool of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia ᵇFisheries Research Institute, FRI Glami Lemi, 71650 Titi, Negeri Sembilan, Malaysia https://orcid.org/0000-0001-8689-1278
  • Dayang Norulfairuz Abang Zaidel ᵃSchool of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia ᶜInstitute of Bioproduct Development, Faculty of Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia https://orcid.org/0000-0003-3043-6713
  • Mohd Noor Mat Isa Malaysia Genome Institute, 43000 Kajang, Selangor, Malaysia https://orcid.org/0000-0001-6426-9832

DOI:

https://doi.org/10.11113/jurnalteknologi.v83.17198

Keywords:

Beta-1,4-galactanase, thermophilic, pectin, galactan, galactooligosaccharides

Abstract

Galactooligosaccharides (GOS) synthesis has received much attention due to its prebiotic function. Beta-1,4-galactanase responsible for the hydrolysis of galactan plays an important role in producing GOS from biodegradation of this pectin component. In this study, beta-1,4-galactanase (BgcGC) from a thermophilic Geobacillus mahadii Geo-05 was heterologously expressed in Escherichia coli (E. coli) and characterized. The optimum temperature of BgcGC was at 60°C and stable from 20-60°C while optimum pH was at 6 and stable from pH 4-10. BgcGC showed high catalytic efficiency towards potato galactan (873.8 ml mg-1 s-1) and lupin galactan (1694.4 ml mg-1 s-1). The activity of BgcGC was not significantly affected with the presence of 100 mM K+, Tween-20 and 2-mercaptoethanol. Application of BgcGC towards pectin-containing galactan oligomer extracted from sweet potato peels resulted in galactose and GOS synthesis as revealed by high performance liquid chromatography analysis. Thus, this enzyme has a potential to be one of the enzyme candidates involves in pectin complex degradation to produce GOS.

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Published

2021-08-01

How to Cite

Ismail, N. F., Abang Zaidel, D. N., & Mat Isa, M. N. (2021). CHARACTERIZATION OF THERMOSTABLE BETA-1,4-GALACTANASE AND ITS APPLICATION IN HYDROLYSIS OF PECTIN FROM SWEET POTATO (Ipomoea batatas (L.) Lam) PEELS . Jurnal Teknologi, 83(5), 57-65. https://doi.org/10.11113/jurnalteknologi.v83.17198

Issue

Section

Science and Engineering