In Vitro Regeneration and Antioxidant Propertiesof Lycium Barbarum L. (Goji)
DOI:
https://doi.org/10.11113/jt.v62.1876Keywords:
In vitro regeneration, antioxidant, DPPH assay, Lycium barbarum L.Abstract
Ethnobotanical studies has documented the consumption of goji by Chinese tribes since ancient times. Goji or scientifically known as Lycium barbarum L. belongs to Solanaceae family and native to some areas of China. The present study was conducted to determine the best explant and corresponding hormonal compositions for Goji in vitro regeneration. In addition, the age and organ of in vitro seedlings with the optimum level of antioxidant activity were also identified. For in vitro regeneration, leaves and nodes were used as explants and cultured on in vitro regeneration media with varying hormonal concentration, combinations of α-napthaleneacetic acid (NAA) and 6-benzyl amino purine (BAP). The optimum combination for in vitro regeneration was found in leaf explants treated with equal concentration of 0.5 mg/L NAA and BAP each. DPPH assay for antioxidant activity screening done on methanolic extracts of samples from two through five month-old in vitro seedlings.shows the highest antioxidant activity in two-month old leaf and stem samples with the EC50 value of 0.08 mg/ml. Indeed, the results revealed that L. barbarum has the potency to be excellently micropropagated that possesses an outstanding antioxidant activity that is essential in phytomedicines.
References
H. J. Feng, Y. C. Jie, P. L. Yun & Z. L. Gui. 2001. The Effect of Lycium Barbarum (Wolfberry) on Antioxidant Activity in the Retina of Diabetic Rats. Retrieved June 3,2010 from http://www.richnature.com.
Q. Luo, Y. Cai, J. Yan, M. Sun & H. Corke. 2004. Hypoglycemic and Hypolipidemic Effects and Antioxidant Activity of Fruit Extracts from Lycium Barbarum. Life Sci. 76(2): 137–149.
H. Zhao, A. Alexeev, E. Chang, G. Greenburg & K. Bojanowski. 2005. Lycium Barbarum Glycoconjugates: Effect on Human Skin and Cultured Dermal Fibroblasts. Phytomedicine. 12: 131–137.
J. Zhu, L. H. Zhao, X. P. Zhao & Z. Chen. 2007. Lycium Barbarum Polysaccharides Regulate Phenotypic and Functional Maturation of Murine Dendritic Cells. Cell Biology International. 31: 615–619.
L. Jing & L. Yin. 2010. Antihyperglycemic activity of polysaccharide from Lycium barbarum. Journal of Medicinal Plants Research. 4(1): 23–26.
G. E. Thomson. 2010. Further Consideration of Asian Medicinal Plants in Treating Common Chronic Diseases in the West. Journal of Medicinal Plants Research. 4(2): 125–130.
J. Pighin. 2003. Your guide to plant cell culture. The Science Creative Quarterly, 4. Retrieved 6 December 2009, from http://www.scq.ubc.ca/your-guide-to-plant-cell-culture/
K. Thaipong, U. Boonprakob, K. Crosby, L. Cisneros-Zevallos & D. H. Byrne. 2006. Comparison of ABTS, DPPH, FRAP, and ORAC Assays for Estimating Antioxidant Activity from Guava Fruit Extracts. Journal of Food Composition and Analysi. 19: 669–675.
O. Azizah, M. J. Abbi-Maleyki, K. W. Kong, I. Amin, A. G. Nawalyah, & A. Ilham. 2010. Epicatechin content and antioxidant capacity of cocoa beans from four different countries. Afr J. Biotechnol. 9(7): 1052–1059.
R. Nurliyana, I. Syed-Zahir, K. Mustapha-Suleiman, M. R. 'Aisyah, & K. Kamarul-Rahim. 2010. Antioxidant Study of Pulps and Peels of Dragon Fruits: A Comparative Study. International Food Research Journal. 2010(17): 367–375.
P. Molyneux. 2004. The use of Free Radical Diphenylpicryl-Hydrayl (DPPH) for Estimating Antioxidant Activity. Songklanarin J. Sci. Technol. 26 (2): 211–219.
N. P. Peteros & M. M. Uy. 2010. Antioxidant and Cytotoxic Activities and Phytochemical Screening of Four Philippine Medicinal Plants. J Med Plants Res, 4(5): 407–414.
Downloads
Published
Issue
Section
License
Copyright of articles that appear in Jurnal Teknologi belongs exclusively to Penerbit Universiti Teknologi Malaysia (Penerbit UTM Press). This copyright covers the rights to reproduce the article, including reprints, electronic reproductions, or any other reproductions of similar nature.
