DUMBO CATFISH (CLARIAS GARIEPINUS) MICROPLASTIC EXPOSURE ON ABNORMALITIES AND LEVEL OF BLOOD COMPONENT USING A COMPLETELY RANDOMIZED DESIGN
DOI:
https://doi.org/10.11113/jurnalteknologi.v85.19764Keywords:
Microplastic, blood structural, abnormality, dumbo catfish, aquaculture.Abstract
Recently, the increase in environmental pollution has been studied by researchers, especially the increase in abnormalities and blood level in aquatic organisms. In this regard, one of the reasons is the presence of plastic causes that are often found in water. The existence of plastic waste in the water is divided into 3 categories, namely macroplastic (>25 mm), mesoplastic (5-25 mm), and microplastic (<5 mm). Microplastics occur from the degradation of macroplastics and mesoplastics in waters. Microplastics in lakes, rivers, and estuaries cause a stress response in the bodies of aquatic organisms including dumbo catfish (Claries gariepinus). The stress response of fish can be seen in the changes in blood components and levels of blood, which are used to determine the condition or health status of fish by counting the number of erythrocytes, leucocytes, hemoglobin, and differential leucocytes. This study aims to analyze the stress response of dumbo catfish caused by microplastic exposure to hematology. This used research method was a completely randomized design (CRD) with one factor and three treatment levels. The results showed that the increase in rearing time was from 0 to 30 days and varied of doses mixture commercial fish feed and microplastic. The results showed the highest erythrocyte value of 2.78(x106) cell/mm3 in 15 days of rearing time by adding mixed commercial feed and microplastics of 15%.
References
Itnawita, H., T. A, A., Khoirul, dan Eriesa 2014. Analisis Kandungan Formaldehid dalam Minuman dengan Kemasan Plastik Polyethylen Tereftalat (PET) yang Beredar di Kodya Pekanbaru. Photon: Jurnal Sain Dan Kesehatan. 4(2): 39-42.
Cordova, M. R., dan Wahyudi, A. J. 2016. Microplastic in the Deep-Sea Sediment of Southwestern Sumatran Waters. Marine Research in Indonesia. 41(1): 27-35.
Huang, J. N., Wen, B., Zhu, J. G., Zhang, Y. S., Gao, J. Z., dan Chen, Z. Z. 2020. Exposure to Microplastics Impairs Digestive Performance, Stimulates Immune Response and Induces Microbiota Dysbiosis in the Gut of Juvenile Guppy (Poecilia reticulata). Science of the Total Environment. 733: 138929.
Hutami, R., Idzni, N., Ranasasmita, R., dan Suprayatmi, M. 2017. DNA Extraction Method for Molecular Detection Gel for Electrophoresis Materials Result. Jurnal Pertanian. 8(2): 106-112.
Klontz, G. W., dan Smith, L. S. 1968. Methods of using Fish as Biological Research Subjects. Methods of Animal Experimentation. 3: 323-383.
Bi, S. H., Jiang, L. L., Doi, L. Y., Zheng, H., Zhang, J., Wang, L. L. 2019. Rh- Incompatible Hemolytic Disease of Newborn in Hefei. World. J. Clin Cases. 7(20): 3202-3207.
Ho, B. T., Roberts, T. K., dan Lucas, S. 2018. An Overview on Biodegradation of Polistirene and Modified Polistirene: the Microbial Approach. Critical Reviews in Biotechnology. 38(2): 308-320.
Prasantini Nahendran, Siti Balkis Budin, Nur Zakiah Mohd Saat, Mohd Faeiz Yusop, Tengku Norita Tengku Yazid, Nur Najmi Mohamad Anuar. 2023. Haemolytic of Newborn Disease-Related Red Blood Cell Alloaantibodies. Jurnal Teknologi. 8591: 1-9.
Hussain, Z., dan Ali, S. 2019. Comparative Study on Breaking Strength of Burnt Clay Bricks Using Novel Based Completely Randomized Design (CRD) Civil Engineering Journal Comparative Study. 5(5).
Preanger, C., Utama, I. H., dan Kardena, I. M. 2016. Gambaran Ulas Darah Ikan Lele di Denpasar Bali. Indonesia Medicus Veterinus. 5(2): 96-103.
Lee, Y. K., & Hur, J. 2020. Adsorption of Microplastic-Derived Organic Matter onto Minerals. Water Research. 187: 116426.
Lusher, A., Hollman, P., dan Mandoza-Hill, J. 2017. Microplastics in Fisheries and Aquaculture. FAO Fisheries and Aquaculture Technical Paper. 615(7).
Bernstam, V. A. 1992. CRC Handbook of Gene Level Diagnostics in Clinical Practice. CRC Press.
López, J., Angelica-López-Téllez, N., dan Rodríguez-Canul, R. 2017. Presence of Infectious Hypodermal and Haematopoietic Necrosis Virus (IHHNV) in Native Shrimps from Southern Mexico. Open Journal of Marine Science. 7(03): 424.
Mead, K. F., dan Borysenko, M. 1984. Surface Immunoglobulin on Granular and Agranular Leukocytes in the Thymus and Spleen of the snapping turtle, Chelydraserpentina. Developmental dan Comparative Immunology. 8(1): 109-120.
Hoffman, R., Benz, E. J., Silberstein, L. E., Heslop, H. E., Weitz, J. I., Anastasi, and Abutalib, S. 2017. Hematology: Basic Principles and Practice. Elsevier Inc. 10.
Arief, M., Fitriani, N., dan Subekti, S. 2014. Pengaruh Pemberian Probiotik Berbeda Pada Pakan Komersial Terhadap Pertumbuhan dan Efisiensi Pakan Ikan Lele Sangkuriang (Clarias sp.). Jurnal Ilmiah Perikanan dan Kelautan. 6(1): 49-53.
M. Marlisa, Diana, H., Yerizam, M., Junaidi, R., and Fadarina. 2020. Dalam Pembuatan Tepung Mocaf (Modified Cassava Flour). Jurnal Polsri. 1(1): 87-91.
Utami, D. P., and Herdina, I. N. 2021. Pengukuran Kualitas Sumber Air Media Pemeliharaan Ikan di Balai Riset Pemuliaan Ikan. Buletin Teknik Litkayasa Akuakultur. 19(1): 19-24.
Adrial, A., Nur, I., dan Yusnaini. 2018. Potensi Tepung Kulit Buah Manggis (Garcinia Mangostana) yang Ditambahkan dalam Pakan terhadap Profil Darah Ikan Komet (Carrasius Auratus). Media Akuatika. 3(3): 689-701.
Rahma, F. W., G. Mahasri dan L. Surmartiwi. 2015. Pengaruh Pemberian Ekstrak Sargassum sp. dengan Pelarut Metanol pada Pakan terhadap Jumlah Eritrosit dan Differensial Leukosit Ikan Lele Dumbo (Clarias gariepinus). Jurnal Ilmiah Perikanan dan Kelautan. 7(2): 213-218.
Arsal, L. M., M. Yuhana, S. Nuryati, dan Alimuddin. 2014. Sintasan Ikan Mas Turunan Ketiga Pembawa marka Cyca-DAB1*05 Ditantang dengan Aeromonas Hydrophila. Jurnal Akuakultur Indonesia. 13(2): 167-178.
Qiang, L., dan Cheng, J. 2021. Exposure to Polistirene Microplastics Impairs Gonads of Zebrafish (Danio rerio). Chemosphere. 263: 128161.
Wootton, R. J., dan Smith, C. 2014. Reproductive Biology of Teleost Fishes. Wiley.
Zheng, M. Z., Richard, J. L., dan Binder, J. 2006. A Review of Rapid Methods for the Analysis of Mycotoxins. Mycopathologia. 161(5): 261-273.
Sapto P. Putro, Nuke Hawariziq, Widowati, Satriyo Adhy 2022. Utilization of Dominant and Opportunistic Taxa of Microbenthic Assemblages Inhabiting Sediments under Fish Farms for the Environmental Status Assessment. Jurnal Teknologi. 84(6): 171-179.
Yu, A., Sun, X., Tang, S., Zhang, Y., Li, M., & Wang, X. 2021. Adsorption Mechanism of Cadmium on Polystyrene Microplastics Containing Hexabromocyclododecane. Environmental Technology & Innovation. 24: 102036.
Zong, X., Zhang, J., Zhu, J., Zhang, L., Jiang, L., Yin, Y., & Guo, H. 2021. Effects of Polystyrene Microplastic on Uptake and Toxicity of Copper and Cadmium in Hydroponic Wheat Seedlings (Triticum aestivum L.). Ecotoxicology and Environmental Safety. 217: 112217.
Witeska, M. 2005. Stress in Fish-hematological and Immunological Effects of Heavy Metals. Electronic Journal of Ichthyology. 1(1): 35-41.
Moseley, P. L. 1997. Heat Shock Proteins and Heat Adaptation of the Whole Organism. Journal of Applied Physiology. 83(5): 1413-1417.
Ekvall, M. T., Lundqvist, M., Kelpsiene, E., Šileikis, E., Gunnarsson, S. B., dan Cedervall, T. 2019. Nanoplastics Formed during the Mechanical Breakdown of Daily-use Polystyrene Products. Nanoscale Advances. 1(3): 1055-1061.
Cerlina, M., Riauwaty, M., & Syawal, H. 2021. Gambaran Eritrosit Ikan Lele Dumbo (Clarias gariepinus) yang Terinfeksi Aeromonas hydrophila dan Diobati dengan Larutan Daun Salam (Syzygium polyantha). Jurnal Perikanan dan Kelautan. 27(1): 105-113.
Chen, C. Y., Lu, T. H., Yang, Y. F., dan Liao, C. M. 2020. Toxicokinetic/toxicodynamic-based Risk Assessment of Freshwater Fish Health Posed by Microplastics at Environmentally Relevant Concentrations. Science of the Total Environment. 144013.
Ding, J., Zhang, S., Razanajatovo, R. M., Zou, H., dan Zhu, W. 2018. Accumulation, Tissue Distribution, and Biochemical Effects of Polistirene Microplastics in the Freshwater Fish Red Tilapia (Oreochromis niloticus). Environmental Pollution. 238: 1-9.
Espinosa, C., Beltrán, J. M. G., Esteban, M. A., dan Cuesta, A. 2018. In Vitro Effects of Virgin Microplastics on Fish Head-kidney Leucocyte Activities. Environmental Pollution. 235: 30-38.
Cong, Y., Jin, F., Tian, M., Wang, J., Shi, H., Wang, Y., dan Mu, J. 2019. Ingestion, Egestion and Post-exposure Effects of Polistirene Microspheres on Marine Medaka (Oryzias melastigma). Chemosphere. 228: 93-100.
Tanbiyaskur, T., Yulisman, Y. and Yonarta, D. 2019. Uji LC50 Ekstrak Akar Tuba dan Pengaruhnya Terhadap Status Kesehatan Ikan Nila (Oreochromis niloticus). Journal of Aquaculture and Fish Health. 8(3): Hal. 129-138.
Thummabancha, K., Onparn, N., dan Srisapoome, P. 2016. Analysis of Hematologic Alterations, Immune Responses and Metallothionein Gene Expression in Nile Tilapia (Oreochromis niloticus) Exposed to Silver Nanoparticles. Journal of Immunotoxicology. 13(6): 909-917.
Azhari, M., Handayani, L., & Nurhayati, N. 2020. Pengaruh Penambahan Arang Aktif Tulang Ikan pada Pakan terhadap Gambaran Darah Ikan Nila (oreochromis niloticus). Jurnal Tilapia. 1(2): 19-27.
Banaee, M., Soltanian, S., Sureda, A., Gholamhosseini, A., Haghi, B. N., Akhlaghi, M., dan Derikvandy, A. 2019. Evaluation of Single and Combined Effects of Cadmium and Micro-plastic Particles on Biochemical and Immunological Parameters of Common Carp (Cyprinus carpio). Chemosphere. 236: 124335.
Muntasiroh, S., Purbomartono, C., & Mulia, D. S. 2020. Kombinasi Ekstrak Rumput Laut Cokelat (Padina sp.) dan Vitamin C Melalui Pakan Terhadap Imun Non-Spesifik Lele Dumbo (Clarias gariepinus). Sainteks. 17(1): 7-17.
Schirinzi, G. F., Llorca, M., Seró, R., Moyano, E., Barceló, D., Abad, E., dan Farré, M. 2019. Trace Analysis of Polistirene Microplastics in Natural Waters. Chemosphere. 236.
Downloads
Published
Issue
Section
License
Copyright of articles that appear in Jurnal Teknologi belongs exclusively to Penerbit Universiti Teknologi Malaysia (Penerbit UTM Press). This copyright covers the rights to reproduce the article, including reprints, electronic reproductions, or any other reproductions of similar nature.
