Effect of 2,4-D on Embryogenic Callus Induction of Malaysian indica Rice (Oryza sativa L.) Cultivars MR123 and MR127
DOI:
https://doi.org/10.11113/jt.v64.2048Keywords:
Malaysian rice, indica, Callus induction, embryogenic, 2, 4- Dicholophenoxyacetic-acid, viabilityAbstract
The aim of this study is to study the effect of various concentrations of 2,4-D on embryogenic callus induction of indica rice MR123 cultivar and MR127 cultivar using mature seeds. Optimal media for induction of callus of both cultivars was MS basal media supplemented with 30g/L sucrose, 500mg/L glutamine and 500mg/L proline supplemented with 2.5 mg/L 2,4-D. The highest percentage of callus induction was 70% and 76% for MR123 and MR127 respectively. Both cultivars produced an embryogenic callus from scutellum after a week in culture with white-yellowish in color. The viability tested using Evans blue show callus obtained was embryogenic. This simple protocol using staining method could be used for screening embryogenic callus before further experiments can be conducted.
References
Abe, T and Futsuhara, Y. 1986. Genotypic Variability for Callus Induction and Plant Regeneration in Rice (Oryza sativa). Theoretical and Applied Genetic. 72(1): 3–10.
Datta, S. K. 2004. Rice Biotechnology: A Need for Developing Countries. AgBioForum. 7(1&2): 31–35.
FAOSTAT. 2012. Food and Agricultural Commodities Production. http://faostat.fao.org.
Fernandez-Da S. R. and Menéndez-Yuffá A. 2006. Viability in Protoplasts and Cell Suspensions of Coffea arabica cv. Catimor. Electronic Journal of Biotechnology. 9: 593–597.
Hou, B. H. and Lin, C. G. 1996. Rapid Optimization of Electroporation Conditions for Soybean and Tomato Suspension Cell Cultures. Plant Physiology. 111(2: Supplement): 166
Huang, C. N., Cornejo, M. J., Bush, D. S. and Jones R. L. 1986. Estimating Viability of Plant Protoplast Using Double and Single Staining. Protoplasma. 135: 80–87.
Kartikayan, A., Pandian, S. T. K. and Ramesh, M. 2009. High Frequency of Plant Regeneration from Embryogenic Callus of Popular Indica Rice (O. sativa L.). Physiology and Molecular Biology of Plants. 15(4): 371–375.
Khaleda L. and Al-Forkan M. 2006. Genotypic Variability in Callus Induction and Plant Regeneration Through Somatic Embryogenesis of Five Deepwater Rice (Oryza sativa L.) cultivars of Bangladesh. African Journal of Biotechnology. 5(16): 1435–1440.
Khanna, H. K. and Raina, S. K. 1999. Agrobacterium-mediated Transformation of Indica Rice Cultivars Using Binary and Super Binary Vectors. Australian journal of Plant Physiology. 26: 311–324.
Kumria, R., Waie, B., Pujni, D. and Rajam, M. V. 2000. Biotechnology of Rice; Present Limitations and Future Prospects. Plant Cell Biotech Mol. Biol. 1: 1–12.
Lee K., Jeon H. and Kim M. 2002. Optimization of a Mature Embryo-Based In Vitro Culture System For High-Frequency Somatic Embryogenesis Callus Induction And Plant Regeneration From Japonica Rice Cultivars. Plant Cell, Tissue and Organ Culture. 71: 237–244.
Murashige, T. and Skoog, F. 1962. A Revised Medium for Rapid Growth and Bioassay with Tobacco Tissue Culture. Physiologia Plantarum. 15(3): 473–497.
Narciso, J. O. and Hattori, K. 2010. Genotypic Differences in Morphology and Ultrastructures of Callus Derived from Selected Rice Varieties. Phillipine Science Letters. 3(1): 59–65.
Ramesh, M., Murugiah, V. and Gupta, A. K. 2009. Efficient Plant Regeneration from Leaf Base Segments of Indica Rice. Indian Journal of Experimental Biology. 47: 68–74.
Silva, L. C. Paiva R., Corrêa Da Silva D. P., Barbosa P., Herrera R. C., Davide L.C. and Duarte De Oliveira Paiva, P. 2012. Characterization of Pro-embryogenic Calli and SomaticEmbryogenesis of Byrsonima. intermedia A. Juss. Journal of Agricultural Science and Technology. 2: 962–970
Smith, B. A., Reider, M. L. and Fletcher, J. S. 1982. Relationship Between Vital Staining and Subculture Growth During the Senescence of Plant Tissue Culture. Plant Physiology. 70: 1228–1230.
Sahoo K. K, Tripathi A. K, Pareek A, Sopory S. K and Singla-Pareek S.L. 2011. An improved Protocol for Efficient Transformation and Regeneration of Diverse indica Rice Cultivars. Plant Method. 7: 49–59.
Swain, D. and De, D. N. 1994. Vital Staining, A Technique for Rapid Screening of Plant Protoplast Viability. Indian Journal Experimental Biology. 32: 501–506.
Syaiful, B. P., Siti, N. A. A., Maheran, A. A., Sariah, M. and Othman, O. 2009. Somatic Embryogenesis from a Scultellar Embryo of Oryza sativa L. var MR219. Pertanika Journal of Tropical Agricultural Science. 32(2): 185–194.
Tey, Y-S and Radam, A. 2011. Demand Patterns of Rice Imports in Malaysia: Implications for Food Security. Food Security. 3: 253–261.
Vega, R., Vasquez, N., Espiniza, A. M. Gatical, A.M. and Valdez-Melara, M. 2009. Histology of Somatic Embryogenesis in Rice (Oryza sativa cv 5272). Revista de Biologia Tropical. 57(1): 141–150.
Visarada, K. B. R. S., Sailaja, M. and Sarma, N. P. 2002. Effect of Callus Induction Media on Morphology of Embryogenic Calli in Rice Genotypes. Biologia Plantarum. 45(4): 495–502.
Widholm, J. M. 1972. The use of Fluorescent Diacetate and Phenosafranine for Determining Viability of Cultured Plant Cell. Stain Technology. 47(4): 89–94.
Zuraida, A. R., Suri, W., Wan Zaliha, W.S., Sreeraramanan, S. 2010. Regeneration of Malaysian indica rice (Oryza sativa) variety MR232 via Optimized Somatic Embryogenesis System. Journal of Phytology. 2(3): 30–38.
Zuraida A. R., Naziah B., and Zamri, Z. 2011. Efficient Plant Regeneration of Malaysian Indica Rice MR219 and 232 Via Somatic Embryogenesis System. Acta Physiologiae Plantarum. 33: 1913–1921.
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