BREAKDOWN OF GLUCOMANNAN ISOLATED FROM AMORPHOPHALLUS MUELLERI: OPTIMIZATION OF ENZYMATIC HYDROLYSIS CONDITIONS
DOI:
https://doi.org/10.11113/jurnalteknologi.v88.25608Abstract
This study investigated the hydrolysis of glucomannan from Amorphophallus muelleri using β-mannanase under various reaction conditions, such as reaction time, pH, temperature and enzyme substrate (E/S) ratio. Response Surface Methodology (RSM) was utilized to identify optimal parameters for maximizing total reducing sugar (TRS) production. The results indicated that the E/S ratio, pH, and reaction time significantly affected hydrolysis efficiency (p < 0.05), while temperature had an insignificant impact (p > 0.05). The ideal conditions were determined to be an E/S ratio of 1:500 (v/v), pH 5.73, 41.5 °C, and 5 h, resulting in a TRS yield of 4.409 ± 0.030 mg/mL, with a difference of merely 1.46% from the expected values. This study demonstrates the viability of A. muelleri as a sustainable resource for oligosaccharides production and confirms that enzymatic hydrolysis coupled with RSM is an effective approach for process optimization.
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