Extraction of 2-Acetyl-1-Pyrroline (2AP) in Pandan Leaves (Pandanus Amaryllifolius Roxb.) Via Solvent Extraction Method: Effect of Solvent

Authors

  • Norzita Ngadi Department of Chemical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia
  • Noor Yahida Yahya Department of Chemical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia

DOI:

https://doi.org/10.11113/jt.v67.2735

Keywords:

Extraction, 2-acetyl-1-pyrroline, pandan leaves, solvent

Abstract

Pandan (Pandanus amaryllifolius Roxb.) leaves are widely used in Malaysia as a source of natural flavoring.  The major compound contributing to the characteristic flavour of Pandan is 2-acetyl-1-pyrroline (2AP). As the consumer requirement for use of natural flavours, extraction of components from natural sources has been sought. In this study, solvent extraction of 2AP from Pandan leaves was performed. The effect of solvent used during extraction process (i.e. methanol, ethanol, propanol) towards the yield of 2AP was investigated. The presence of 2AP was determined using GCMS. The results obtained showed that ethanol was the best solvent to extract 2AP from Pandan leaves compared to methanol as higher 2AP peak arises from ethanol chromatogram.  However there is no 2AP detected when propanol was used as solvent.  It is believed that polarity of the solvent plays an important role in the extraction of 2AP.

References

Mohsin, H. F., I. Abdul Wahab, and J. F. W. Abdullah. 2010. A Derivative of Furan-2,3-Diol from Pandan Extract. International Conferences on Science and Social Research (CSSR 2010). 5-7 December. Kuala Lumpur, Malaysia: 169–171.

Routray, W. and K. Rayaguru. 2010. Chemical Constituents and Post-Harvest Prospects of Pandanus amaryllifolius Leaves: A Review. Food Reviews International. 26: 230–245.

Yahya, F., T. Lu, R.C.D. Santos, P.J. Fryer, and S. Bakalis. 2010. Supercritical Carbon dioxide and Solvent Extraction of 2-acetyl-1-pyrroline from Pandan Leaf: The Effect of Pre-Treatment. Journal of Supercritical Fluids. 55: 200–207.

Chong, H. Z., R. Asmah, A. Abdah Md, M. A. Norjahan Banu, O. Fauziah, and C. L. Gwendoline Ee. 2010. Chemical Analysis of Pandan Leaves (Pandanus Amaryllifolius). International Journal of Natural Product and Pharmaceutical Sciences. 1: 7–10.

Buttery, G. R., L. C. Ling, B. O. Juliano, and J. G. Turnbaugh. 1983. Cooked Rice Aroma and 2-acetyl-1-pyrroline. Journal of Agricultural and Food Chemistry. 31(4): 823–826.

Buttery, G. R., L. C. Ling, and B. O. Juliano, 1985. U. S. Patent No. 4,522,838.

Laohakunjit, N. and O. Kerdchoechuen. 2007. Aroma Enrichment and the Change During Storage of Non-Aromatic Milled Rice Coated with Extracted Natural Flavor. Food Chemistry. 101: 339–344.

Buttery, G. R., B. O. Juliano, and L. C. Ling. 1982. Identification of Rice Aroma Compound 2-acetyl-1-pyrroline in Pandan Leaves. Chemistry and Industry. 23: 478.

Lin, C. F., T. C. Y. Hsieh, and B. J. Hoff. 1989. Identification and Quantification of Popcorn-Like Aroma in Louisiana Aromatic Della Rice (Oryza sativa I.). Journal of Food Science. 55: 1466–1467.

Itani, T., M. Tamaki, Y. Hayata, T. Fushimi, and K. Hashizume. 2004. Variation of 2-acetyl-1-pyrroline Concentration in Aromatic Rice Grains Collected in the Same Region in Japan and Factors Affecting Its Concentration. Plant Production Science. 7(2): 178–183.

Paule, C. M. and J. Powers. 1989. Sensory and Chemical Examination of Aromatic and Nonaromatic Rices. Journal of Food Science. 54: 343–347.

Apintanapong, M. and A. Noomhorm. 2003. The Use of Spray Drying to Microencapsulate 2-acetyl-1-pyrroline, a Major Flavour Component of Aromatic Rice. International Journal of Food Science and Technology. 38: 95–102.

Mohamad, S. N. H. 2011. Bacterial Cellulose Membrane Coated with Pyrroline for Hexavalent Chromium Removal in Wastewater Treatment. Master Degree. Universiti Teknologi Malaysia, Skudai.

Markoma, M., M. Hasan, W.R. Wan Daud, H. Singh, and J. Md Jahim. 2007. Extraction of Hydrolysable Tannins from Phyllanthus niruri Linn.: Effects of Solvents and Extraction Methods. Separation and Purification Technology. 52: 487–496.

Laohakunjit, N. and A. Noomhorm. 2004. Supercritical Carbon dioxide Extraction of 2-acetyl-1-pyrroline and Volatile Components from Pandan Leaves. Flavour and Fragrance Journal. 19: 251–259.

Bhattacharjee, P., A. Kshirsagar, and R.S. Singhal. 2005. Supercritical Carbon Dioxide Extraction of 2-acetyl-1-pyrroline from Pandanus amaryliffolius Roxb. Food Chemistry. 91: 255–259.

Downloads

Published

2014-02-28

How to Cite

Extraction of 2-Acetyl-1-Pyrroline (2AP) in Pandan Leaves (Pandanus Amaryllifolius Roxb.) Via Solvent Extraction Method: Effect of Solvent. (2014). Jurnal Teknologi, 67(2). https://doi.org/10.11113/jt.v67.2735