Supercritical Fluid Extraction of Swietenia mahagoni Seed: Antioxidant and Antimicrobial Activities

Authors

  • Hartati Hartati Department of Bioprocess Engineering, Faculty of Chemical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia
  • Liza Md Salleh Department of Bioprocess Engineering, Faculty of Chemical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia
  • Ahmad Ramdan Ismail Department of Bioprocess Engineering, Faculty of Chemical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia
  • Mohd. Azizi Che Yunus Centre of Lipid Engineering and Applied Research (CLEAR), Faculty of Chemical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia
  • Azila Abd. Aziz Department of Bioprocess Engineering, Faculty of Chemical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia

DOI:

https://doi.org/10.11113/jt.v67.2799

Keywords:

Supercritical fluid extraction (SFE), Swietenia mahagoni, antioxidant, antimicrobial

Abstract

Swietenia mahagoni seeds have been used as folk medicine for a treatment of hypertension, malaria and diabetes. This research was conducted to obtain extracts from the Swietenia mahagoni using supercritical fluid extraction (SFE) with pure CO2 solvent, in order to evaluate the high- pressure method in terms of process yield and biological activity. The various conditions namely flow rate of CO2 were set up at 2, 3, and 4 ml/min; at constant pressures (P) and temperature (T). The sample extracts obtained by SFE with CO2 flow rate of 4 ml/min showed the highest percentage yield (19.67%) compared to the others. The antioxidant potential of the extracts was evaluated by the DPPH method and Folin- Ciocalteu method. The solvent flow rate of 2 ml/min gave the lowest percentage of yield, but good results in antioxidant activity and total phenolic content. The antimicrobial activity of the extracts against Gram-positive (Bacillus subtilis, Staphylococcus aureus) and Gram-negative (Escherichia coli) was evaluated based on the inhibition zone using disc diffusion assay. These results ensured that Swietenia mahagoni seed extract had inhibitory effects on the growth of Bacillus subtilis, Staphylococcus aureus and Escherichia coli at concentration of 100 mg/ml.

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Published

2014-03-15

Issue

Vol. 67 No. 4: Special Issue on Sustainable Green Separation Towards Future Challenges Vol. 1

Section

Science and Engineering

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Copyright of articles that appear in Jurnal Teknologi belongs exclusively to Penerbit Universiti Teknologi Malaysia (Penerbit UTM Press). This copyright covers the rights to reproduce the article, including reprints, electronic reproductions, or any other reproductions of similar nature.

How to Cite

Supercritical Fluid Extraction of Swietenia mahagoni Seed: Antioxidant and Antimicrobial Activities. (2014). Jurnal Teknologi, 67(4). https://doi.org/10.11113/jt.v67.2799