Stability Study of Water-in-Oil Emulsion Containing Anthocyanins from Red Cabbage

Authors

  • Nur Shakira Sahat Department of Bioprocess Engineering, Faculty of Chemical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor Malaysia
  • Dayang Norulfairuz Abang Zaidel Department of Bioprocess Engineering, Faculty of Chemical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor Malaysia
  • Ida Idayu Muhamad Department of Bioprocess Engineering, Faculty of Chemical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor Malaysia
  • Muhd Nazrul Hisham Zainal Alam Department of Bioprocess Engineering, Faculty of Chemical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor Malaysia

DOI:

https://doi.org/10.11113/jt.v69.3163

Keywords:

Anthocyanin, emulsion, margarine, red cabbage, stability

Abstract

One of the main factors in the formulation and control of water-in-oil emulsion is the development of desirable solid-to-liquid ratios, achieved by hydrogenation and blending. Formulation of water-in-oil emulsion with bioactive compound could provide additional value and stability to the emulsion for food application for example in margarine. The objectives of this study were to formulate water-in-oil emulsions i.e. margarine containing bioactive compound (anthocyanins) extracted from red cabbage (Brassica oleracea L.) and to study the storage and stability characteristics of the emulsions. The anthocyanins were extracted using hot water extraction method at 60°C with three replications, and encapsulation of anthocyanins was performed using microwave technique to study the dispersion and stability of anthocyanins in the emulsions. In this study, water-in-oil emulsions were formulated with three different oil-to-aqueous ratios (75:25, 80:20 and 85:15) at 60°C for 15 minutes during blending and at 0°C for 30 minutes during crystallization process. The stability of the formulated emulsion was determined by using Solid Fat Content analysis, thermal and phase stability analysis. Total concentration of anthocyanin extracted from red cabbage was 152.33±2.0 mg/L. After 14 days of storage, the emulsion stability was achieved at oil-to-aqueous ratio of 85:15 and with addition of encapsulated anthocyanins. This formulation produced stable emulsion with high melting completion temperature and low onset crystallization temperature.  

References

Vaisey-Genser, M. 2003. Composition and Analysis of Margarine. The University of Manitoba: Elsevier Science Ltd. 3714.

Ushibuko, F., Y. and R. L. Cunha. 2014. Stability mechanism of liquid water-in-oil emulsions. Food Hydrocolloids. 34: 145–153.

Beltran-Orozco, M. C., T. G. Oliva-Coba, T. Gallardo-Velazquez and G. Osorio-Revilla. 2009. Ascorbic Acid, Phenolic Content, and Antioxidant Capacity Of Red, Cherry, Yellow And White Types Of Pitaya Cactus Fruit (Stenocereus stellatus Riccobono). Agrociencia. 43: 153–162.

Dembitsky, V. M., S. Poovarodom, H. Leontowicz, M. Leontowicz, S. Vearasilp, S. Trakhtenberg and S. Gorinstein. 2011. The Multiple Nutrition Properties of Some Exotic Fruit: Biological Activity and Active Metabolites. Food Research International. 44: 1671–1701.

Mohd-Esa, N., F. S. Hern, A. Ismail and C. L. Yee. 2010. Antioxidant Activity in Different Parts of Roselle (Hibiscus Sabdariffa L.) Extracts and Potential Exploitation of the Seeds. Food Chemistry. 122: 1055–1060.

Markakis, P., G. E. Livingstone and G. R. Fillers. 1957. Quantitative Aspects of Strawberry Pigments Degradation. Food Research. 22: 117–130.

Duangmal, K., B. Saicheuaa and S. Sueeprasan. 2004. Roselle Anthocyanins as a Natural Food Colourants and Improvement of its Colour Stability. AIC 2004 Colour and Paints, Interim Meeting of the International Colour Associations. 155–158.

Ersus, S. and U. Yurdagel. 2006. Microencapsulation of Anthocyanin Pigments of Black Carrot (Daucuscarota L.) by Spray Drier. Journal of Food Engineering. 80: 805–812.

Looi, M. L. 2008. Nano Encapsulation of Anthocyanins from Brassica Oleracea. Universiti Teknologi Malaysia. MSc Thesis.

Abbasi, S. and S. Rahimi. 2008. Microwave-assisted Encapsulation of Citric Acid using Hydrocolloids. International Journal of Food Science and Technology. 43: 1226–1232.

Young, D. K. and C. V. Morr. 1996. Microencapsulation Properties of Gum Arabic and Several Food Proteins: Spray-Dried Orange Oil Emulsion Particles. Journal of Agriculture and Food Chemistry. 44: 1314–1320.

Saadi, S., A. A. Ariffin, H. M. Ghazali, M. S. Abdulkarim, C. B. Huey and M. S. Miskandar. 2012. Crystallisation Regime of w/o Emulsion [e.g. Multipurpose Margarine] Models During Storage. Food Chemistry. 133: 1485–1493.

Knoth A., I. Scherze and G. Muschiolik. 2005. Stability of Water-in-Oil-Emulsions Containing Phosphatidylcholine-depleted Lecithin. Food Hydrocolloids. 19: 635–640.

Hui, K. H. 2009. Microwave-assisted Encapsulation of Anthocyanins from Roselle (Hibicus Sabdarifa L.) and its Thermal Stability Analysis. Universiti Teknologi Malaysia. MSc Thesis.

Rao, R., K. U. Sankar, K. Sambaiah and B. R. Lokesh. 2001. Differential Scanning Calorimetric Studies on Structured Lipids from Coconut Oil Trigryceride Containing Stearic Acid. European Food Research Technology. 212: 334–343.

Dollimore, D. 1996. Thermal analysis. Analytical Chemistry, 68: 63–71.

Tan, Y., Xu, K., Niu, C., Liu, C., Li, Y., Wang, P. and Binks, B. P. 2014. Triglyceride-water Emulsions Stabilised by Starch-based Nanoparticles. Food Hydrocolloids. 36: 70–75.

Downloads

Published

2014-06-25

How to Cite

Stability Study of Water-in-Oil Emulsion Containing Anthocyanins from Red Cabbage. (2014). Jurnal Teknologi (Sciences & Engineering), 69(4). https://doi.org/10.11113/jt.v69.3163