Fractionation of Labisia Pumila using Solid-Phase Extraction for Extraction of Gallic Acid
DOI:
https://doi.org/10.11113/jt.v69.3176Keywords:
Solid phase extraction, gallic acid, fractionationAbstract
Phenolics are the most ample secondary metabolites of plants and have drawn increasing attention in their applications in health supplement and functional cosmetic. This is due to their strong antioxidant properties and their marked effects in the inhibition of several oxidative stress associated metabolic disorder such as cancer and aging. In Labisia pumila, gallic acid (3,4,5-Trihydroxybenzoic acid) was found to be one of the most abundant secondary metabolite. It has been proposed as a biomarker in cosmetic and food supplement applications. In this research the identification and isolation of gallic acid were carried out. Different sorbent of solid-phase extraction (SPE) column were used for the fractionation of Labisia pumila var Alata water extract and the amount of gallic acid present in the fractions were quantified. The aim is to identify the better sorbent-solvent combination in SPE that result in higher recovery of gallic acid from Labisia pumila var Alata water extract. A range of solvent mixtures of 20%, 40%, 60% and 80% methanol (Me Oh)-water was used. Gallic acid was successfully extracted and identified from Labisia pumila. From this discovery, C18 silica reversed phase solid phase extraction column could extracts highest gallic acid from Labisia pumila var Alata water extract. Retention of analyte from  the sample from second elution using 80% methanol-water Solvent may be used as the best method to extract the compound from Labisia Pumila var Alata since it has been reported to have various bioactivities such as high antioxidant activity and cytotoxic against many diseases. Using C18 silica reversed phase in SPE as a fractionation and extraction of gallic acid from Labisia pumila var Alata can be achieved for application purpose or future studies.
References
Pattiram, P. D., O. Lasekan, C. P. Tan and I. S. M. Zaidul. 2011. Identification of The Aroma-active Constituents of the Essential Oils of Water Dropwort (Oenanthe Javanica) and Kacip Fatimah. 18(3): 1021–1026.
Choi, H., D. Kim, J. W. Kim, S. Ngadiran, M. R. Sarmidi and C. S. Park. 2010. Labisia Pumila Extract Protects Skin Cells from Photoaging Caused by UVB Irradiation. Journal Biosci. Bioeng. 109(3): 291–6.
Yusoff, M. M. and A. C. Iwansyah. 2011. Comparative Evaluation of Total Phenolics and Free Radical Scavenging Activity of Aqueous Extracts of Labisia pumila var.alata from Malaysia and Indonesia. No. ICBFS. 4–8.
Norhaiza, M., M. Maziah and M. Hakiman. 2009. Antioxidative Properties of Leaf Extracts of a Popular Malaysian Herb. Labisia Pumila. 3(4): 217–223.
Zhang, Q. and M. Ye. 2009. Chemical Analysis of the Chinese Herbal Medicine Gan-Cao (Licorice). 1216: 1954–1969.
Jaafar, H. Z. E., M. H. Ibrahim and E. Karimi. 2012. Phenolics and Flavonoids Compounds, Phenylanine Ammonia Lyase and Antioxidant Activity Responses to Elevated CO₂ in Labisia Pumila (Myrisinaceae). Molecules. 17(6): 6331–47.
Karimi, E., H. Z. E. Jaafar and S. Ahmad. 2011. Phytochemical Analysis and Antimicrobial Activities of Methanolic Extracts of Leaf, Stem and Root from Different Varieties of Labisa Pumila Benth. Molecules. 16(6): 4438–50.
Ibrahim, M. H., H. Z. E. Jaafar, E. Karimi and A. Ghasemzadeh. 2012. Primary, Secondary Metabolites, Photosynthetic Capacity and Antioxidant Activity of the Malaysian Herb Kacip Fatimah (Labisia Pumila Benth) Exposed to Potassium Fertilization under Greenhouse Conditions. International Journar of Molecular Science. 13(11): 15321–42.
Arabbi, P. R., M. I. Genovese and F. M. Lajolo. 2004. Flavonoids in Vegetable Foods Commonly Consumed in Brazil and Estimated Ingestion by the Brazilian Population. Journal of Agricultural and Food Chemical. 52(5): 1124–1131.
Chua, L. S., N. A. Latiff, S. Y. Lee, C. T. Lee, M. R. Sarmidi and R. A. Aziz. 2011. Flavonoids and Phenolic Acids from Labisia Pumila (Kacip Fatimah). Food Chemistry. 127(3): 1186–1192.
Kole, P. L., G. Venkatesh, J. Kotecha and R. Sheshala. 2011. Recent Advances in Sample Preparation Techniques for Effective Bioanalytical Methods. Biomedical Chromatography. 25(1–2): 199–217.
Picariello, G., F. Bonomi, S. Iametti, P. Rasmussen, C. Pepe, S. Lilla and P. Ferranti. 2011. Proteomic and Peptidomic Characterisation of Beer: Immunological and Technological Implications. Food Chemistry. 124(4): 1718–1726.
Hernández-Ledesma, B., B. Miralles, L. Amigo, M. Ramos and I. Recio. 2005. Identification of Antioxidant and ACE-inhibitory Peptides in Fermented Milk. Journal of the Science of Food and Agriculture. 85(6): 1041–1048.
Muguruma, M., M. A. Ahhmed, K. Katayama, S. Kawahara, M. Maruyama and T. Nakamura. 2009. Identification of Pro-drug Type ACE Inhibitory Peptide Sourced from Porcine Myosin B: Evaluation of its Antihypertensive Effects in Vivo. Food Chemistry. 114 (2): 516–522.
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