Isolation of Pectin from Nephrolepis Biserrata Leaves at Different Extraction Time

Authors

  • Halifah Pagarra Department of Bioprocess Engineering Faculty of Chemical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia
  • Roshanida A. Rahman Department of Bioprocess Engineering Faculty of Chemical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia
  • Mazura Jusoh Department of Chemical Engineering Faculty of Chemical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia

DOI:

https://doi.org/10.11113/jt.v69.3196

Keywords:

Nephrolepis biserrata leaves, fractionation, Lignocellulose content, extraction time, pectin

Abstract

Based on a previous test (fractionation) on Nephrolepis biserrata, lignocellulose contains 34.27% of pectin and oligosacharides, 1.78% of hemicellulose, 20.66% of cellulose and 43.29% of lignin. The optimum time for extraction of pectin from Nephrolepis biserrata leaves was investigated. Extraction time of 60, 90, 120 minutes at pH 1.5 and temperature of 80°C were found to significantly affect the extraction of pectin from Nephrolepis biserrata leaves. Maximum pectin yield is 15.8%, which was obtained in sulphuric acid solution of pH 1.5 at 80°C for 120 minutes. 

References

W.G.T. Willats, L. McCarney, W. Mackei, J.P. Knox. 2001. Pectin: Cell Biology for Functional Analysis. Plant. Mol. Bio. 47: 9‒27.

Y. Eiji and K. Osamu. 2007. Extraction and Characterization of the Pectic Substances from Japanese Pepper (Zanthoxylum piperitum dc.) Fruit. Int. J. Food Prop. 10: 505‒513.

D. Constenla and J. E. Lozano. 2003. Latin Am. Appl. Res. 33: 91‒96.

Z.U. Rehman. 2004. Utilization of Mango Peels as a Source of Pectin. J. Chem. Soc. Pakistan. 26:73‒76.

S. Levigne, M.C. Ralet, J.-F. Thibault. 2002. Characterisation of Pectins Extracted from Fresh Sugar Beet Under Different Conditions Using an Experimental Design. Carbohyd. Polym. 49: 145–153.

Budiyono and Yulianingsih. 2008. Effect of Temperature and Time of Extraction on Characters of Pectin Extracted from Siam Citrus Pulp (Citrus nobilis L). Jurnal Pasca Panen 5: 37‒44.

C. Mollea, F. Chiampo, R. Conti. 2008. Extraction and Characterization of Pectin from Cocoa Husks: A Preliminary Study. Food Chem. 107: 1353–1356.

W. Sijin, C. Fang, W. Jihong, W. Zhengfu, L. Xiaojun, H. Xiaosong. 2007. Optimization of Pectin Extraction Assisted by Microwave from Apple Pomace Using Response Surface Methodology. J. Food Eng. 78: 693–700.

O.J. Babayemi, M.A. Bamikole, A.B. Omojola. 2006. Evaluation of the Nutritive Value and Free Choice Intake of Two Aquatic (Nephrolepis biserrata and Spirodelapolyrhyza) by Westafrican Dwarft Goats. Trop. Subtrop. Agroecosyst. 6: 15‒21.

H.H. Yeoh, Y.C. Wee. 1998. Total Amino Acid Compositions of Tropical Ferns and Gymnosperms. Asian Journal of Tropical. 3: 13‒18.

Ratin Datta, 1978. Acidogenic Fermentation of Lignocellulose-Acid Yield and Conversion of Components. Biotechnol. Bioeng. 23: 2167‒2170.

N. Berardini, M. Knödler, A. Schieber, R. Carle. 2005. Utilization of Mango Peels as a Source of Pectin and Polyphenol. Innov. Food Sci. Emerg. Tech. 6: 442‒452.

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Published

2014-07-02

How to Cite

Isolation of Pectin from Nephrolepis Biserrata Leaves at Different Extraction Time. (2014). Jurnal Teknologi (Sciences & Engineering), 69(5). https://doi.org/10.11113/jt.v69.3196