Rigid and Conductive Dual Nanoprobe for Single Cell Analysis
DOI:
https://doi.org/10.11113/jt.v69.3304Keywords:
Electrical Nano probe, finite element method, microfluidic, single cell analysisAbstract
Electrical property characterization of a single cell can be used to infer about its physiological condition, e.g. cell viability. Due to that, a dual nanoprobe-microfluidic system for electrical properties measurement of single cells has been proposed. This paper is concerned about the mechanical and electrical characterizations of the dual nanoprobe. Electrical and mechanical characterizations were conducted to measure the resistance and the strength of the dual nanoprobe for five different metals i.e. Aluminium, Copper, Silver, Tungsten, and Zinc using finite element approach. From the findings, Tungsten’s nanoprobe has the highest strength while the resistance values for the five materials are not significantly different. Therefore, Tungsten is selected as the most recommended metal for the dual nanoprobe. We also performed single cell electrical measurement to test the functionality of the sensor. This work provides general information of the nanoprobe which can be used as a framework in other applications involving Nano devices i.e. cell surgery and drug delivery.
References
Ahmad, M. R., Nakajima, M., Kojima, M., Kojima, S., Homma, M., and Fukuda, T. 2012. Nanofork for Single Cells Adhesion Measurement via ESEM-Nanomanipulator System. IEEE Transactions on NanoBioscience. 11(1): 70–78.
Ahmad, M. R., Nakajima, M., Kojima, S., Homma, M., and Fukuda, T. 2008. In Situ Single Cell Mechanics Characterization of Yeast Cells Using Nanoneedles Inside Environmental SEM. IEEE Transactions on Nanotechnology. 7(5): 607–616.
Ahmad, M. R., Nakajima, M., Kojima, S., Homma, M., and Fukuda, T. 2010. Nanoindentation Methods to Measure Viscoelastic Properties of Single Cells Using Sharp, Flat, and Buckling Tips Inside ESEM. IEEE Transactions on NanoBioscience. 9(1): 12–23.
Chen, J., Li, J., and Sun, Y. 2012. Microfluidic Approaches for Cancer Cell Detection, Characterization, and Separation. Lab on a Chip. 12(10): 1753–1767.
Chen, J., Zheng, Y., Tan, Q., Zhang, Y. L., Li, J., Geddie, W. R., et al. 2011. A Microfluidic Device for Simultaneous Electrical and Mechanical Measurements on Single Cells. Biomicrofluidics. 5(1): 014113.
Chia-Feng, L., Jen-Yu, J., Ming-Kun, C., Ya-Chun, C., Pin-Chian, W., and Ling-Sheng, J. 2011. Single Cell Impedance Analysis and Electrical Characterization in Micro-fluidic Device. In IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS). 121–126.
Asami, K. 2002. Characterization of Biological Cells by Dielectric Spectroscopy. Journal of Non-Crystalline Solids. 305(1–3): 268–277.
Bot, C. and Prodan, C. 2009. Probing the Membrane Potential Of Living Cells By Dielectric Spectroscopy. European Biophysics Journal. 38(8) 1049–1059.
Caselli, F., Bisegna, P., and Maceri, F. 2010. EIT-Inspired Microfluidic Cytometer for Single-Cell Dielectric Spectroscopy. Journal of Microelectromechanical Systems. 19(5): 1029–1040.
Fricke, H., Schwan, H. P., Li, K. A. M., and Bryson, V. 1956. A Dielectric Study of the Low-Conductance Surface Membrane in E. coli. Nature. 177(4499): 134–135.
Hywel, M., Tao, S., David, H., Shady, G., and Nicolas, G. G. 2007. Single Cell Dielectric Spectroscopy. Journal of Physics D: Applied Physics. 40(1): 61–70.
Mihai, C.-M., Mehedintu, M., and Gheorghiu, E. 1996. The Derivation of Cellular Properties from Dielectric Spectroscopy Data. Bioelectrochemistry and Bioenergetics. 40(2): 187–192.
Prodan, C., Mayo, F., Claycomb, J. R., J. H. Miller, J., and Benedik, M. J. 2004. Low-frequency, Low-field Dielectric Spectroscopy of Living Cell Suspensions. Journal of Applied Physics. 95(7): 3754–3756.
Schwan, H. P. 1984. Electrical and Acoustic Properties of Biological Materials and Biomedical Applications. Biomedical Engineering. 31(12): 873–877.
Dalton, C., Goater, A. D., Burt, J. P. H., and Smith, H. V. 2004. Analysis of Parasites by Electrorotation. Journal of Applied Microbiology. 96(1): 24–32.
Lu, Q., Terray, A., Collins, G. E., and Hart, S. J. 2012. Single Particle Analysis Using Fluidic, Optical and Electrophoretic Force Balance in a Microfluidic System. Lab on a Chip. 12(6): 1128–1134.
Medoro, G., Manaresi, N., Leonardi, A., Altomare, L., Tartagni, M., and Guerrieri, R. 2003. A Lab-on-a-chip for Cell Detection and Manipulation. IEEE Sensors Journal. 3(3): 317–325.
Matthews, B. and Judy, J. W. 2006. Design and Fabrication of a Micromachined Planar Patch-clamp Substrate with Integrated Microfluidics for Single-cell Measurements. Journal of Microelectromechanical Systems. 15(1): 214–222.
Cho, Y. H., Yamamoto, T., Sakai, Y., Fujii, T., and Beomjoon, K. 2006. Development of Microfluidic Device for Electrical/Physical Characterization of Single Cell. Journal of Microelectromechanical Systems. 15(2): 287–295.
Ahmad, M. R., Nakajima, M., Fukuda, T., Kojima, S., and Homma, M. 2009. Single Cells Electrical Characterizations Using Nanoprobe via ESEM-nanomanipulator System. In 9th IEEE Conference on Nanotechnology. 589–592.
Ahmad, M. R., Nakajima, M., Kojima, M., Kojima, S., Homma, M., and Fukuda, T. 2012. Instantaneous and Quantitative Single Cells Viability Determination Using Dual Nanoprobe Inside ESEM. IEEE Transactions on Nanotechnology 11(2): 298–306.
Bonora, A. and Mares, D. 1982. A Simple Colorimetric Method for Detecting Cell Viability in Cultures of Eukaryotic Microorganisms. Current Microbiology. 7(4): 217–221.
Rubinsky, B., and Huang, Y. 2005. Cell Viability Detection using Electrical Measurement. United States Patent, 2005.
Zheng, Y., Shojaei-Baghini, E., Wang, C., and Sun, Y. 2013. Microfluidic Characterization of Specific Membrane Capacitance and Cytoplasm Conductivity of Single Cells. Biosensors and Bioelectronics. 42(1): 496–502.
Bianchi, H. and Fernández-Prini, R. 1993. The Conductivity of Dilute Electrolyte Solutions: Expanded Lee and Wheaton Equation for Symmetrical, Unsymmetrical and Mixed Electrolytes. Journal of Solution Chemistry. 22(6): 557–570.
Fichtner, W. 2008. Overview of Technology Computer-Aided Design Tools and Applications in Technology Development, Manufacturing and Design. Journal of Computational and Theoretical Nanoscience. 5(6): 1089–1105.
Arnst, M. and Ghanem, R. 2009. Probabilistic Electromechanical Modeling of Nanostructures with Random Geometry. Journal of Computational and Theoretical Nanoscience. 6(10): 2256–2272.
Ghasemi-Nejhad, M. N. and Askari, D. 2005. Mechanical Properties Modeling of Carbon Single-Walled Nanotubes: A Finite Element Method. Journal of Computational and Theoretical Nanoscience. 2(2): 298–318.
Kalamkarov, A. L., Veedu, V. P., and Ghasemi-Nejhad, M. N. 2005. Mechanical Properties Modeling of Carbon Single-Walled Nanotubes: An Asymptotic Homogenization Method. Journal of Computational and Theoretical Nanoscience. 2(1): 124–131.
Jafari, A., Khatibi, A. A., and Mashhadi, M. M. 2012. Evaluation of Mechanical and Piezoelectric Properties of Boron Nitride Nanotube: A Novel Electrostructural Analogy Approach. Journal of Computational and Theoretical Nanoscience. 9(3): 461–468.
Gere, J. M. 2001. Mechanics of Material. USA: Brooks/Cole.
Sulaiman, A. H. M. and Ahmad, M. R. 2012. Modeling and Simulation of Novel Method of Single Cell Viability Detection via Electrical Measurement Using Dual Nanoprobes. In International Conference on Enabling Science and Nanotechnology (ESciNano). 1–2.
Stenson, J. D., Hartley, P., Wang, C., and Thomas, C. R. 2011. Determining the Mechanical Properties of Yeast Cell Walls. Biotechnology Progress. 27(2): 505–512.
Raicu, V., Raicu, G., and Turcu, G. 1996. Dielectric Properties of Yeast Cells as Simulated by the Two-shell Model. Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1274(3): 143–148.
Hölzel, R. and Lamprecht, I. 1992. Dielectric Properties of Yeast Cells as Determined by Electrorotation. Biochimica et Biophysica Acta (BBA) - Biomembranes. 1104(1): 195–200.
Walker, G. M. 2009. Yeasts. In Encyclopedia of Microbiology (Third Edition), S. Editor-in-Chief: Moselio, Ed., ed Oxford: Academic Press. 478–491.
Sidorov, I. A., Blumenthal, R., and Dimitrov, D. S. 2006. A Model of Drug Delivery to Normal and Cancer Cells by Antibody-Targeted Nanoliposomes. Journal of Computational and Theoretical Nanoscience. 3(3): 405–411.
Downloads
Published
Issue
Section
License
Copyright of articles that appear in Jurnal Teknologi belongs exclusively to Penerbit Universiti Teknologi Malaysia (Penerbit UTM Press). This copyright covers the rights to reproduce the article, including reprints, electronic reproductions, or any other reproductions of similar nature.
