Ciri Morfologi, Fisiko-Kimia dan Kerintangan Dua Titisan Padi Kemandulan Jantan Sitoplasma (CMS) Baru

Authors

  • Elixon Sunian Pusat Penyelidikan Padi dan Tanaman Industri, MARDI Seberang Perai, Pulau Pinang, Malaysia
  • Mohd. Solihen Jamal Stesen Penyelidikan Padi Hibrid, MARDI Perlis, Tambun Tulang, Perlis, Malaysia
  • Asfaliza Ramli Pusat Penyelidikan Padi dan Tanaman Industri, MARDI Seberang Perai, Pulau Pinang, Malaysia
  • Othman Omar Pusat Penyelidikan Padi dan Tanaman Industri, MARDI Seberang Perai, Pulau Pinang, Malaysia
  • Habibbudin Hashim Pusat Penyelidikan Padi dan Tanaman Industri, MARDI Seberang Perai, Pulau Pinang, Malaysia
  • Siti Norsuha Misman Pusat Penyelidikan Padi dan Tanaman Industri, MARDI Seberang Perai, Pulau Pinang, Malaysia
  • Maisarah Mohd Saad Pusat Penyelidikan Padi dan Tanaman Industri, MARDI Seberang Perai, Pulau Pinang, Malaysia

DOI:

https://doi.org/10.11113/jt.v70.3530

Keywords:

Yield, Cytoplasmic male sterile, backcross, hybrid rice, physical and chemical qualities, pest and disease, maintainer, restorer

Abstract

CMS (cytoplasmic male sterile) line is one of the most important hybrid parents in hybrid rice production technology using Three-Line systems. Test-crosses for selection of candidate local maintainer lines were conducted using 24 rice varieties and two CMS-WA (wild abortive) lines IR78374A/B and 1A/B. The F1 of the two combinations, namely IR78374A/P519 and 1A/MR243 had less than 10% spikelet fertility (6.4 and 7.2%, respectively), indicated that P519 and MR243 are partially maintainers and could be used for the development of new locally adapted CMS lines. As a results, two CMS lines, namely 0025A/B and 0047A/B were generated after 5 to 6 successive backcrossing of IR78374A with P519 and 1A with MR243. The CMS 0025A/B and 0047A/B lines had maturation age which is comparable to other commonly grown inbred varieties. The percentage of stigma exertion rate for CMS 0025A/B and 0047A/B were 17.9 and 21.3%, respectively, lower than that of IR78374A/B (43.2%). Both showed poor out-crossing rate. CMS 0025A/B and 0047A/B are classified as long and slender grain. They had intermediate amylose content. Their gel consistency was hard, comparable to that of MR263 (medium).  CMS 0025A/B and 0047A/B lines were also moderately resistant to foliar blast disease and brown planthopper infestation.

References

Biju, S., Thiyagarajan, K. and Manonmani, S. 2007. Cytoplasmic Male Sterile Lines for Hybrid Rice Production. Journ. of app. Sci. Res. 3(10): 935–937.

Eckardt, N. A`. 2006. CytoplasmicMale Sterility and Fertility Restoration. The Plant Cell. 18: 515–517.

Feng, W., Wuge L., Yisheng H. and Zhenrong L. 2009. Improving Grain Quality in Hybrid Rice. In: Accelerating Hybrid Rice Development. Edited by F. Xie and B. Hardy. Publisher IRRI. 366–375.

Fu, F. H, Wang, F., Huang, W. J, Peng, H. P, Wu, Y. Y. and Huang, D. J. 1994. Genetic Analysis on Grain Characters in Hybrid Rice. Acta Agron. Sin. 20(1): 39–45.

Golam, F., Mohamad, O., Hadzim, K. and Meisner C. A. 2004. Inheritance of Kernel Elongation in Rice. Int. J. Agri. Biol. 6(5):813– 815.

Gu, M., Lie, Q., Yan, C., and Tang S. 2009. Grain Quality of Hybrid Rice: Genetic Variation and Molecular Improvement. In: Accelerating Hybrid Rice Development. Edited by F. Xie and B. Hardy. Publisher IRRI. 345–356.

Guok, H. P. 1994. Hybrid Rice Research in Malaysia. In: Virmani S.S, editor. Hybrid Rice Technology: New Developments and Future Prospects. Manila (Philippines): International Rice Research Institute. 207–212.

Gupta, P.K, Rustgi, S. and Kumar, N. 2006. Genetic and Molecular Basis of Grain Size and Grain Number and Its Relevance to Grain Productivity in Higher Plants. Genome. 49: 565–571.

IRRI. 1996. 4th edition. Standard Evaluation System for Rice (SES), Manila, Philippines.

IRRI (International Rice Research Institute). http://www.knowledgebank. irri.org/rkb/index.php/rice-milling Retrieved August 07 2013.

Jena, K. K, Mackill, D. J. 2008. Molecular Markers and Their Use in Marker-assisted Selection in Rice. Crop Sci. 48: 1266–1276.

Jena, K. K. and Suh, J. P. 2009. New Genes for Brown Planthopper, Blast, and Bacterial Blight Resistance, and Marker-assisted Breeding in Rice. In: Accelerating Hybrid Rice Development. Edited by F. Xie and B. Hardy. Publisher IRRI. 149–158.

Li, S. Q, Yang, D. C. and Zhu, Y. G. 2007. Characterization and Use of Male Sterility in Hybrid Rice Breeding. J Integr. Plant Biol. 49(6): 791–804.

Li, Z.B. 1980. A Preliminary Discussion About the Classification of Male Sterile Lines of Rice in China. Acta Agronomica Sinica. 6: 17–26.

Mao, C., Shi, Y., Wei, S, Song, Z., Zhou, H., Wang, W., Xie, L. and Liu, B. 2009. How to Increase Use Efficiency of Rice Hybrids and Their Parental Lines. In: Accelerating Hybrid Rice Development. Edited by F. Xie and B. Hardy. Publisher IRRI. 159–168.

Mei, Q., Xie, R., Liu, C., Zhou, X. and Zhu, Y. 2011. Breeding and Characterization of Homokaryotic Heteroplasmic male Sterile Lines in Rice (Oryza Sativa). Pak. J. Bot. 43(3): 1691–1694.

Mohamad, O., Guok, H. P and Othman, O. 1987. Hybrid Rice Breeding in Malaysia. Malaya Appl.Biol. 16: 129-138.

Nematzadeh, G. A. and Kiani, G. 2010. Genetic Analysis of Fertility Restoration Genes for Watype Cytoplasmic Male Sterility in Iranian Restorer Rice Line DN-33-18. African Journal of Biotechnology. 9(38): 6273–6277.

Oka, H.T. and Morishima, H. 1967. The Ancestors of Cultivad Rice and Their Evolution. Japan: Department of Applied Genetics, National Institute of Genetic. 145–145.

Saad, A., Yahya, H., Nik Mohd Noor, N. S. Azmi, M., Badrulhadza, A., Siti Norsuha, M., Azimah, A.K. dan Sivapragasam A. 2012. Pengurusan Perosak Bersepadu: Prinsip, konsep dan Amalan. Bab dalam buku: Pengurusan Perosak Bersepadu Tanaman Padi Kearah Pengeluaran Lestari. Penyunting: Saad, A., Badrulhadza, A., Sariam, O., Azmi, M., Yahya, H., Siti Norsuha, M. dan Maisarah, M.S. Terbitan MARDI (Malaysia Research and Development Institute). 2–46.

Salgotra, R. K., Gupta, B. B and Singh, S. 2009. Evaluation of Various Floral Traits in Some Rice CMS Lines that Influence Seed Setting Under Subtropical Condition. SABRAO J. of Breeding and Genetic. 41(2): 115–122.

Sattari, M., Kathiresan, A., Gregorio, G.B. and Virmani, S. S. 2008. Comparative Genetic Analysis and Molecular Mapping of Fertility Restoration Genes for WA, Dissi, and Gambiaca Cytoplasmic Male Sterility Systems in Rice. Euphytica. 160: 305–315.

Taillebois, J. and Guimaraes, E. 1986. Improving Outcrossing Rate in Rice (Oryza sativa L.). n: Hybrid Rice. Proceedings of the International Symposium on Hybrid Rice 6-10 October 1986 Changsha, Hunan, China. 175–180.

Tan, X., Hong, R., Tan, Y., Jin S., Xu, H., Guo, A., Chen, L., Huang, D., Zhang, Z., Tao, G., Wang, X., Zhang, S., Shi, C., and Li, Z. 2009. Research on the Development of CMSD1-based Japonica Hybrid Rice with High Yield Potential for the Yun-Gui Plateau. In: Accelerating Hybrid Rice Development. Edited F. Xie and B. Hardy. Publisher IRRI. 131–138.

Tan, Y. F., Xing, Y. Z., Li, J. X., Yu, S. B., Xu, C. G and Zhang, Q. 2000. Genetic Bases of Appearance Quality of Rice Grains in Shanyou 63, An Elite Rice Hybrid. Theor. Appl. Genet. 101: 823–829.

Unnevehr, L. J., Duff, B. and Juliano, B. O. 1992. Consumer Demand for Rice Grain Quality: Introduction and Major Findings. In: Consumer Demand for Rice Grain Quality. Terminal Report of IDRC Projects National Grain Quality (Asia). Edited by L. J. Unnevehr, B. Duff, and B. O. Juliano. Publisher International Rice Research Institute. 5–20.

Virmani, S. S, Choudhury, R. C, Khush, G. S. 1981. Current Outlook on Hybrid Rice. Oryza. 18: 87–92.

Virmani, S. S., Viraktamath, B. C., Casal, C. L., Toledo, R. S., Lopez, M. T. and Manalo, J. O. 1997. Hybrid Rice Breeding Manual. International Rice Research Institute (IRRI). Los Banos, Philippines. 37–43.

Xie, F. 2009. Priorities of IRRI Hybrid Rice Breeding. In: Accelerating Hybrid Rice Development. Edited F. Xie and B. Hardy. Publisher International Rice Research Institute. 49–62.

Yuan, L. P. 1995. Current Status of Hybrid Rice in China and Future Strategies for 21st Century. In: Ahmed M. I., Viraktamath B. C. (Eds). Hybrid Rice Seed Production Technology. Directorate of Rice Research, Hyderabad, India.

Zhou, P. H., Tan, Y. F., He, Y. Q., Xu, C. G. and Zhang, Q. 2003. Simultaneous Improvement for Four Quality Traits Of Zhenshan 97, An Elite Parent Of Hybrid Rice, by Molecular Marker-assisted Selection. Theor. Appl. Genet. 106: 326–331.

Zhou, J. H., Wang, J. L., Xu, J. C., Lei, C. L. and Ling, Z. Z. 2004. Identification and Mapping of a Rice Blast Resistance Gene Pi-G(T) In The Cultivar Guangchangzhan. Plant Pathol. 53: 191–196.

Zhu, Z., Min J., Zhang, B., Chen, N., and Wang, D. 2009. Hybrid Rice Quality in China. In: Accelerating Hybrid Rice Development. Edited by F. Xie and B. Hardy. Publisher IRRI. 357–366.

Zhu, Y. G. 1979. Studies on Male Sterile Lines of Rice with Different Cytoplasms. Acta Agronomica Sinica. 5: 29–38.

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Published

2014-09-19

How to Cite

Ciri Morfologi, Fisiko-Kimia dan Kerintangan Dua Titisan Padi Kemandulan Jantan Sitoplasma (CMS) Baru. (2014). Jurnal Teknologi (Sciences & Engineering), 70(6). https://doi.org/10.11113/jt.v70.3530