A PRELIMINARY STUDY ON MOSQUITO LARVICIDAL EFFICACY OF ROTENONE EXTRACTED FROM MALAYSIA Derris sp.
DOI:
https://doi.org/10.11113/jt.v76.3953Keywords:
Derris elliptica, rotenone, liquid crude extract, mosquito larvae, biological activity, mortalityAbstract
Rotenone is a bio-active compound extracted from Derris elliptica (locally known as ‘Tuba’ plant). It has long been used as bio-pesticide, which is more environmental friendly than the commercially available pesticides and has the potential to be used in eliminating mosquito larvae. Therefore, the objective of this study is to determine the mosquito larvicidal activity (LC50) through the usage of liquid crude extract of Derris plant root. The rotenone liquid crude extract was extracted using normal soaking extraction (NSE) method. Two different solvent ratios were used to extract rotenone namely: (A) methyl chloride: methanol (1:1) and (B) methyl chloride: methanol (1:9). The extracts were concentrated using rotary evaporator at 40 oC with vacuum pressure of 800 mbar prior to the reverse-phase high performance liquid chromatography analysis (RP-HPLC) and biological activity (LC50) study. Next, the diluted extracts were subjected to the biological activity treatment for 6 hrs. The results showed that the concentrated liquid crude extracts of methyl chloride: methanol (1:1) which contained the highest rotenone content produced the lowest treatment concentration of 0.024 mg/ml to achieve 50% mortality within 3 hrs of treatment (p<0.05). The rapid mortality (as indicated by the LC50 value) of the mosquitoes’ larvae against rotenone extracted from Derris plant roots has proven that it has the potential to be used as larvicide to control vector-borne diseases especially from mosquitoes.
References
World Health Organization. World Malaria Report 2010. <http://www.who.int/malaria/world_malaria_report_2010/en/index.html>; 2011 [Accessed: 18.01.2014].
Mace, K. E., M. F. Lynch, J. R. MacArthur, S. P. Kachur, L. Slutsker, R. W. Steketee, T. Popovic. 2011. Grand Rounds: The Opportunity for and Challenges to Malaria Eradication. Morbidity and Mortality Weekly Report CDC. 60(15): 476-80.
Cantrell, C. L., F. E. Dayan and S. O. Duke. 2013. Natural Products as Sources for New Pesticides. J. Nat. Prod. 75(6): 1231-1242.
Becker N., D. Petric, M. Zgomba, C. Boase, M. Madon, C. Dahl, A. Kaiser. 2010. Mosquitoes and Their Control. Springer, New York.
Suraphon, V. and M. Manthana, 2001. Effects of root extract from Derris (Derris elliptica Benth) on Mortality And Detoxification Enzyme Levels in the Diamondback Moth Larvae (Plutella xylostella Linn.). Am. Ent. 8: 100-120.
Radad, K., W.-D. Rausch, G. Gille. 2006. Rotenone Induces Cell Death In Primary Dopaminergic Culture By Increasing ROS Production And Inhibiting Mitochondrial Respiration. Neurochem. Intern. 49: 379-386.
Zubairi, S .I., M. R. Sarmidi and R. A. Aziz. 2014. The Effects of Raw Material Particles Size, Types of Solvents and Solvent-To-Solid Ratio on the Yield of Rotenone Extracted From Derris Elliptica Roots. Sains Malaysiana. 43(5): 707-713.
Zubairi, S. I., M. R. Sarmidi and R. A. Aziz. 2014. Identification of Bio-active Constituents from Derris Elliptica Liquid Crude Extract Using Vacuum Liquid Chromatography. Adv. Environ. Biol. 8(2): 437-440.
Zubairi, S. I., M. R. Sarmidi and R. A. Aziz. 2014. A Preliminary Study of Rotenone Exhaustive Extraction Kinetic From Derris Elliptica Dried Roots Using Normal Soaking Extraction (NSE) Method. Adv. Environ. Biol. 8(4): 910-915.
Zubairi, S. I., M. R. Sarmidi and R. A. Aziz. 2015. A Thermal Degradation (Thermolysis) Study of Rotenone Extracted from Derris elliptica Roots Using Reverse-Phase High Performance Liquid Chromatography (RP-HPLC). Sains Malaysiana. 44(1): 161-166.
Zubairi, S. I., M. R. Sarmidi and R. A. Aziz. 2014. Bio-active Constituents of Rotenoids Resin Extracted from Derris Elliptica Roots: Comparison Between Local Plant Extract and SAPHYR (France) Cube Resin. Adv. Environ. Biol. 8(4): 904-909.
Armitage, P., G. Berry and J. N. S. Matthews. 2001. Statistical Methods in Medical Research. 4th Edition. Wiley-Blackwell Scientific Publications, Oxford.
Finney, D. J. 2009. Probit Analysis. 1st Edition (digitally printed version). Cambridge University Press, New York.
Zubairi, S. I., M. R. Sarmidi and R. A. Aziz. 2014. Biological Activity on the Extract of Derris elliptica: An Optimization Approach to Investigate the Effect of Processing Parameters on Mortality of Artermia salina. Adv. Environ. Biol. 8(10): 9018-924.
Ottoboni, M. A. 1991. The Dose Makes The Poison. 2nd edn, Van Nostrand Reinhold, New York.
Cheung, Y. K. 2011. Dose Finding by the Continual Reassessment Method. 1st Edition. Chapman & Hall/CRC Biostatistics Series.
Farnesi, L. C., J. M. Brito, J. G. Linss, M. Pelajo-Machado and D. Valle. 2012. Physiological and Morphological Aspects of Aedes aegypti Developing Larvae: Effects of the Chitin Synthesis Inhibitor Novaluron. PLoS ONE. 7(1): e30363. DOI:10.1371/journal.pone.0030363.
Cabizza, M., A. Angioni, M. Melis, M. Cabras, C. V. Tuberoso and P. Cabras. 2004. Rotenone and Rotenoids in Cube Resins, Formulations and Residues on Olives. J. Agric. Food Chem. 52: 288-293.
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