EVALUATION OF THREE RNA EXTRACTION METHODS FROM THREE CULTIVARS OF MALAYSIAN UPLAND RICE

Authors

  • Shahkila Mohd Arif Department of Biotechnology and Medical Engineering, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia
  • Nyuk Ling Ma School of Fundamental Science, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia
  • Alina Wagiran Department of Biotechnology and Medical Engineering, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia
  • Zaidah Rahmat Department of Biotechnology and Medical Engineering, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia Innovation Center in Agrotechnology for Advanced Bioprocessing (ICA), Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia

DOI:

https://doi.org/10.11113/jt.v78.4564

Keywords:

Malaysian upland rice, RNA extraction, guanidine isothiocyanate, purity, downstream applications

Abstract

Rice (Oryza sativa L.) can be divided into two major categories, which is upland rice and lowland rice. Apart from being the staple food for more than half of the world population, it is also known as model plant for functional genomics study. However, it possess high amount of starch and polysaccharide that makes the isolation of good quality RNA for downstream purposes often a difficult task. While there are many studies being carried out for lowland rice extraction, none has been reported for upland rice. This study is the first to report on evaluation of three RNA extraction methods for three Malaysian upland rice cultivars in order to determine the best method to isolate high grade RNA. The result obtained demonstrated that good quality RNA in terms of integrity, purity and quality can be isolated from young leaves of these cultivars  by using guanidine isothiocyanate based extraction method that is fast, simple and efficient and had been proven suitable for further downstream applications. 

 

 

Author Biographies

  • Shahkila Mohd Arif, Department of Biotechnology and Medical Engineering, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia
    Department of Biotechnology and Medical Engineering, PhD student
  • Nyuk Ling Ma, School of Fundamental Science, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia
    School of Fundamental Science, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia
  • Alina Wagiran, Department of Biotechnology and Medical Engineering, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia
    Department of Biotechnology and Medical Engineering, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia
  • Zaidah Rahmat, Department of Biotechnology and Medical Engineering, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia Innovation Center in Agrotechnology for Advanced Bioprocessing (ICA), Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia

    Department of Biotechnology and Medical Engineering, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia

    Innovation Center in Agrotechnology for Advanced Bioprocessing (ICA), Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia

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Published

2015-12-22

Issue

Vol. 78 No. 1: January 2016

Section

Science and Engineering

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How to Cite

EVALUATION OF THREE RNA EXTRACTION METHODS FROM THREE CULTIVARS OF MALAYSIAN UPLAND RICE. (2015). Jurnal Teknologi, 78(1). https://doi.org/10.11113/jt.v78.4564