• Faiznur Mohd Fuad School of Chemical Engineering, Universiti Sains Malaysia, Engineering Campus, 14300 Nibong Tebal, Seberang Perai, Penang, Malaysia
  • Mashitah Mat Don School of Chemical Engineering, Universiti Sains Malaysia, Engineering Campus, 14300 Nibong Tebal, Seberang Perai, Penang, Malaysia




Calophyllum inophyllum, seed oil, ultrasonic-assisted extraction, optimization


Calophyllum inophyllum (C. inophyllum) is a plant known as “Penaga Laut†in Malaysia. Its seeds contain valuable oil that serve as a potential alternative sources for vegetable oil. In this study, C. inophyllum seeds oil was extracted using an ultrasonic-assisted extraction (UAE) technique. The optimization of extraction parameters namely different types of solvent, extraction time, ultrasonic power, extraction temperature and liquid to solid (L/S) ratio were performed using one-factor-at-a-time (OFAT) method. The optimum extraction conditions obtained were n-hexane as a solvent, extraction time 20 min, ultrasonic power 210 W, extraction temperature 40°C and L/S ratio 20 ml/g, with an oil yield 55.44 ± 0.53 %.


Hien, H. M., P. W. Heng, N. V. Thi, N. K. Q. Cu, and V. T. B. Hue. 2011. Development and Validation of a GC-MS Method for Rapid Determination of Calophyllolide in Calophyllum inophyllum L.: A Quality Control Approach. Chinese Journal of Natural Medicines. 9(6): 429-434.

Fattah, I. M. R., H. H. Masjuki, M. A. Kalam, M. A. Wakil, A. M. Ashraful, and S. A. Shahir. 2014. Experimental Investigation Of Performance And Regulated Emissions Of A Diesel Engine With Calophyllum Inophyllum Biodiesel Blends Accompanied By Oxidation Inhibitors. Energy Conversion and Management. 83(0): 232-240.

Praveena, C., R. S. Swaroopa, and C. Veeresham. 2013. Phytochemical Investigation of Calophyllum inophyllum Linn. Natural Products Chemistry & Research. 1(4): 1-4.

Chavan, S. B., R. R. Kumbhar, and R. B. Deshmukh. 2013. Callophyllum Inophyllum Linn (“honneâ€) Oil, A Source For Biodiesel Production. Research Journal of Chemical Sciences. 3(11): 24-31.

Ha, M. H., V. T. Nguyen, K. Q. C. Nguyen, E. L. Cheah, and P.W. Heng. 2009. Antimicrobial Activity Of Calophyllum Inophyllum Crude Extracts Obtained By Pressurized Liquid Extraction. Asian Journal of Traditional Medicines. 4(4): 141-146.

Ong, H. C., T. M. I. Mahlia, H. H. Masjuki, and R. S. Norhasyima. 2011. Comparison of palm oil, Jatropha curcas and Calophyllum inophyllum for biodiesel: A review. Renewable and Sustainable Energy Reviews. 15(8): 3501-3515.

Jahirul, M. I., J. R. Brown, W. Senadeera, N. Ashwath, C. Laing, J. Leski-Taylor, and M. G. Rasul. 2013. Optimisation of Bio-oil Extraction Process from Beauty Leaf (Calophyllum inophyllum) Oil Seed as a Second Generation Biodiesel Source. Procedia Engineering. 56(0): 619-624.

Friday, J. B. and D. Okano. 2006. Species Profiles for Pacific Island Agroforestry: Calophyllum Inophyllum (kamani). Hawaii, USA: Permanent Agriculture Resources (PAR).

Dalvi, S., S. Sonawane, and R. Pokharkar. 2012. Preparation of Biodiesel of Undi seed with In-situ Transesterification. Leonardo Electronic Journal of Practices and Technologies. 11(20): 175-182.

Spino, C., M. Dodier, and S. Sotheeswaran. 1998. Anti-HIV Coumarins From Calophyllum Seed Oil. Bioorganic & Medicinal Chemistry Letters. 8(0): 3475-3478.

Yimdjo, M. C., A. G. Azebaze, A. E. Nkengfack, A. M. Meyer, B. Bodo, and Z.T. Fomum. 2004. Antimicrobial And Cytotoxic Agents From Calophyllum inophyllum. Phytochemistry. 65(0): 2789-2795.

Venkanna, B. K. and R. C. Venkataramana. 2009. Biodiesel Production And Optimization From Calophyllum inophyllum linn oil (honne oil) – A Three Stage Method. Bioresource Technology. 100(21): 5122-5125.

Tiwari, S. 2008. Plants: A Rich Source of Herbal Medicine. Journal of Natural Products. 1(0): 27-35.

Avelar, M. H. M., D. M. J. Cassimiro, K. C. Santos, R. C. C. Domingues, H. F. de Castro, and A. A. Mendes. 2013. Hydrolysis of Vegetable Oils Catalyzed By Lipase Extract Powder From Dormant Castor Bean Seeds. Industrial Crops and Products. 44(0): 452-458.

Trindade, A. S. N., A. F. Dantas, D. C. Lima, S. L. C. Ferreira, and L. S. G. Teixeira. 2015. Multivariate Optimization Of Ultrasound-Assisted Extraction For Determination Of Cu, Fe, Ni And Zn In Vegetable Oils By High-Resolution Continuum Source Atomic Absorption Spectrometry. Food Chemistry. 185(0): 145-150.

Straccia, M. C., F. Siano, R. Coppola, F. L. Cara, and M. G. Volpe. 2012. Extraction and Characterization of Vegetable Oils from Cherry Seed by Different Extraction Processes. Chemical Engineering Transactions. 27(0): 391-396.

Nair, M. K. M., P. Vasudevan, and K. Venkitanarayanan. 2005. Antibacterial Effect Of Black Seed Oil On Listeria Monocytogenes. Food Control. 16(5): 395-398.

Cordova-Albores, L. C., M. Y. Rios, L. L. Barrera-Necha, and S. Bautista-Baños. 2014. Chemical Compounds Of A Native Jatropha Curcas Seed Oil From Mexico And Their Antifungal Effect On Fusarium oxysporum f. sp. gladioli. Industrial Crops and Products. 62(0): 166-172.

Niu, L., J. Li, M.-S. Chen, and Z.-F. Xu. 2014. Determination Of Oil Contents In Sacha Inchi (Plukenetia Volubilis) Seeds At Different Developmental Stages By Two Methods: Soxhlet Extraction And Time-Domain Nuclear Magnetic Resonance. Industrial Crops and Products. 56(0): 187-190.

Mediratta, P. K., K. K. Sharma, and S. Singh. 2002. Evaluation Of Immunomodulatory Potential Of Ocimum Sanctum Seed Oil And Its Possible Mechanism Of Action. Journal of Ethnopharmacology. 80(1): 15-20.

Badea, G., I. Lăcătuşu, N. Badea, C. Ott, and A. Meghea. 2015. Use Of Various Vegetable Oils In Designing Photoprotective Nanostructured Formulations For UV Protection And Antioxidant Activity. Industrial Crops and Products. 67(0): 18-24.

Melo-Espinosa, E. A., R. Piloto-Rodríguez, L. Goyos-Pérez, R. Sierens, and S. Verhelst. 2015. Emulsification Of Animal Fats And Vegetable Oils For Their Use As A Diesel Engine Fuel: An Overview. Renewable and Sustainable Energy Reviews. 47(0): 623-633.

Bhuiya, M. M. K., M. G. Rasul, M. M. K. Khan, N. Ashwath, A. K. Azad, and M. A. Hazrat. 2014. Second Generation Biodiesel: Potential Alternative to-edible Oil-derived Biodiesel. Energy Procedia. 61(0): 1969-1972.

Takase, M., T. Zhao, M. Zhang, Y. Chen, H. Liu, L. Yang, and X. Wu. 2015. An Expatiate Review Of Neem, Jatropha, Rubber And Karanja As Multipurpose Non-Edible Biodiesel Resources And Comparison Of Their Fuel, Engine And Emission Properties. Renewable and Sustainable Energy Reviews. 43(0): 495-520.

Ullah, K., M. Ahmad, Sofia, F. A. Qureshi, R. Qamar, V. K. Sharma, S. Sultana, and M. Zafar. 2015. Synthesis And Characterization Of Biodiesel From Aamla Oil: A Promoting Non-Edible Oil Source For Bioenergy Industry. Fuel Processing Technology. 133(0): 173-182.

Nicolau, A., R. M. Mariath, and D. Samios. 2009. Study Of The Properties Of Polymers Obtained From Vegetable Oil Derivatives By Light Scattering Techniques. Materials Science and Engineering: C. 29(2): 452-457.

Alam, M., D. Akram, E. Sharmin, F. Zafar, and S. Ahmad. 2014. Vegetable Oil Based Eco-Friendly Coating Materials: A Review Article. Arabian Journal of Chemistry. 7(4): 469-479.

Asbahani, A. E., K. Miladi, W. Badri, M. Sala, E. H. A. Addi, H. Casabianca, A. E. Mousadik, D. Hartmann, A. Jilale, F. N. R. Renaud, and A. Elaissari. 2015. Essential Oils: From Extraction To Encapsulation. International Journal of Pharmaceutics. 483(1-2): 220-243.

Chou, S.-T., C.-P. Lai, C.-C. Lin, and Y. Shih. 2012. Study Of The Chemical Composition, Antioxidant Activity And Anti-Inflammatory Activity Of Essential Oil From Vetiveria zizanioides. Food Chemistry. 134(1): 262-268.

Raut, J. S. and S. M. Karuppayil. 2014. A Status Review On The Medicinal Properties Of Essential Oils. Industrial Crops and Products. 62(0): 250-264.

Rabiah Nizah, M. F., Y. H. Taufiq-Yap, U. Rashid, S. H. Teo, Z. A. Shajaratun Nur, and A. Islam. 2014. Production Of Biodiesel From Non-Edible Jatropha Curcas Oil Via Transesterification Using Bi2O3–La2O3 Catalyst. Energy Conversion and Management. 88(0): 1257-1262.

Martins, I. M., M. F. Barreiro, M. Coelho, and A. E. Rodrigues. 2014. Microencapsulation Of Essential Oils With Biodegradable Polymeric Carriers For Cosmetic Applications. Chemical Engineering Journal. 245(0): 191-200.

Kyarimpa, C. M., S. Böhmdorfer, J. Wasswa, B. T. Kiremire, I. O. Ndiege, and J. D. Kabasa. 2014. Essential oil and composition of Tagetes minuta from Uganda. Larvicidal activity on Anopheles gambiae. Industrial Crops and Products. 62(0): 400-404.

Uquiche, E., M. Jeréz, and J. Ortíz. 2008. Effect Of Pretreatment With Microwaves On Mechanical Extraction Yield And Quality Of Vegetable Oil From Chilean Hazelnuts (Gevuina avellana Mol). Innovative Food Science & Emerging Technologies. 9(4): 495-500.

Liu, W., Y.-J. Fu, Y.-G. Zu, M.-H. Tong, N. Wu, X.-L. Liu, and S. Zhang. 2009. Supercritical Carbon Dioxide Extraction Of Seed Oil From Opuntia dillenii Haw. And Its Antioxidant Activity. Food Chemistry. 114(0): 334-339.

Qu, X.-J., Y.-J. Fu, M. Luo, C.-J. Zhao, Y.-G. Zu, C.-Y. Li, W. Wang, J. Li, and Z.-F. Wei. 2013. Acidic pH Based Microwave-Assisted Aqueous Extraction Of Seed Oil From Yellow Horn (Xanthoceras sorbifolia Bunge). Industrial Crops and Products. 43(0): 420-26.

Eller, F. J., S. C. Cermak, and S. L. Taylor. 2011. Supercritical Carbon Dioxide Extraction Of Cuphea Seed Oil. Industrial Crops and Products. 33(0): 554-557.

Ruttarattanamongkol, K., S. Siebenhandl-Ehn, M. Schreiner, and A. M. Petrasch. 2014. Pilot-Scale Supercritical Carbon Dioxide Extraction, Physico-Chemical Properties And Profile Characterization Of Moringa Oleifera Seed Oil Incomparison With Conventional Extraction Methods. Industrial Crops and Products. 58(0): 68-77.

Gai, Q.-Y., J. Jiao, F.-Y. Wei, M. Luo, W. Wang, Y.-G. Zu, and Y.-J. Fu. 2013. Enzyme-Assisted Aqueous Extraction Of Oil From Forsythia Suspense Seed And Its Physicochemical Property And Antioxidant Activity. Industrial Crops and Products. 51(0): 274-278.

Fiori, L., V. Lavelli, K. S. Duba, P. S. C. S. Harsha, H. B. Mohamed, and G. Guella. 2014. Supercritical CO2 Extraction Of Oil From Seeds Of Six Grape Cultivars:Modeling Of Mass Transfer Kinetics And Evaluation Of Lipid Profiles And Tocol Contents. Journal of Supercritical Fluids. 94(0): 71-80.

Guo, Y. X., J. Han, D. Y. Zhang, L. H. Wang, and L. L. Zhou. 2013. Aqueous two-Phase System Coupled With Ultrasound For The Extraction Of Lignans From Seeds Of Schisandra chinensis (turcz.) Baill. Ultrasonics Sonochemistry. 20(0): 125-132.

Rombaut, N., R. Savoire, B. Thomasset, T. Bélliard, J. Castello, É.V. Hecke, and J.-L. Lanoisellé. 2014. Grape Seed Oil Extraction: Interest Of Supercritical Fluid Extraction And Gas-Assisted Mechanical Extraction For Enhancing Polyphenol Co-Extraction In Oil. Comptes Rendus Chimie. 17(0): 284-292.

Wang, L. J. 2010. Advances In Extraction Of Plant Products In Nutraceutical Processing. In Handbook Of Nutraceuticals: Volume II: Scale Up, Processing And Automation. Boca Raton, FL: CRC Press, Taylor & Francis.

Zhu, K.-X., X.-H. Sun, and H.-M. Zhou. 2009. Optimization Of Ultrasound-Assisted Extraction Of Defatted Wheat Germ Proteins By Reverse Micelles. Journal of Cereal Science. 50(2): 266-271.

Vetal, M. D., V. G. Lade, and V. K. Rathod. 2013. Extraction Of Ursolic Acid From Ocimum Sanctum By Ultrasound: Process Intensification And Kinetic Studies. Chemical Engineering and Processing: Process Intensification. 69(0): 24-30.

Bimakr, M., R. Abdul Rahman, F. S. Taip, N. M. Adzahan, M. Z. Sarker, and A. Ganjloo. 2012. Optimization Of Ultrasound-Assisted Extraction Of Crude Oil From Wintermelon (Benincasa Hispida) Seed Using Response Surface Methodology And Evaluation Of Its Antioxidant Activity, Total Phenolic Content And Fatty Acid Composition. Molecules. 17(0): 11748-11762.

Samaram, S., H. Mirhosseini, C. P. Tan, and H. M. Ghazali. 2014. Ultrasound-assisted Extraction And Solvent Extraction Of Papaya Seed Oil: Crystallization And Thermal Behavior, Saturation Degree, Color Andoxidative Stability. Industrial Crops and Products. 52(0): 702-708.

Rathod, S. S. and V. K. Rathod. 2014. Extraction Of Piperine From Piper Longum Using Ultrasound. Industrial Crops and Products. 58(0): 259-264.

Zhu, C.-p., X.-c. Zhai, L.-q. Li, X.-x. Wu, and B. Li. 2015. Response surface Optimization Of Ultrasound-Assisted Polysaccharides Extraction From Pomegranate Peel. Food Chemistry. 177(0): 139-146.

Khan, M. K., M. Abert-Vian, A.-S. Fabiano-Tixier, O. Dangles, and F. Chemat. 2010. Ultrasound-assisted extraction of polyphenols (flavanone glycosides) from orange (Citrus sinensis L.) peel. Food Chemistry. 119(2): 851-858.

Ghitescu, R.-E., I. Volf, C. Carausu, A.-M. Bühlmann, I.A. Gilca, and V.I. Popa. 2015. Optimization Of Ultrasound-Assisted Extraction Of Polyphenols From Spruce Wood Bark. Ultrasonics Sonochemistry. 22(0): 535-541.

Jovanovic-Malinovska, R., S. Kuzmanova, and E. Winkelhausen. 2015. Application Of Ultrasound For Enhanced Extraction Of Prebiotic Oligosaccharides From Selected Fruits And Vegetables. Ultrasonics Sonochemistry. 22(0): 446-453.

Stanisavljević, I. T., M. L. Lazić, and V. B. Veljković. 2007. Ultrasonic Extraction Of Oil From Tobacco (Nicotiana Tabacum L.) Seeds. Ultrasonics Sonochemistry. 14(5): 646-652.

Samaram, S., H. Mirhosseini, C. P. Tan, and H. M. Ghazali. 2013. Ultrasound-Assisted Extraction (UAE) and Solvent Extraction of Papaya Seed Oil: Yield, Fatty Acid Composition and Triacylglycerol Profile. Molecules. 18(0): 12474-12487.

Abdullah, M. and A.B. Koc. 2013. Kinetics of Ultrasound-Assisted Oil Extraction From Black Seed (Nigella sativa). Journal of Food Processing and Preservation. 37(5): 814-823.

Fang, X., J. Wang, Y. Wang, X. Li, H. Zhou, and L. Zhu. 2014. Optimization Of Ultrasonic-Assisted Extraction Of Wedelolactone And Antioxidant Polyphenols from Eclipta prostrate L Using Response Surface methodology. Separation and Purification Technology. 138(0): 55-64.

Hathurusingha, S., N. Ashwath, and D. Midmore. 2011. Periodic variation In Kernel Oil Content And Fatty Acid Profiles Of Calophyllum Inophyllum L.: A Potential Biodiesel Feedstock In Australia. Biomass and Bioenergy. 35(8): 3448-3452.

Nde, D. B., D. Boldor, and C. Astete. 2015. Optimization Of Microwave Assisted Extraction Parameters Of Neem (Azadirachta Indica A. Juss) Oil Using The Doehlert’s Experimental Design. Industrial Crops and Products. 65(0): 233-240.

Bamgboye, A. I. and S. E. Adebayo. 2012. Seed Moisture Dependent On Physical And Mechanical Properties Of Jatropha Curcas. Journal of Agricultural Technology. 8(1): 13-26.

Abdullah, M. H. R. O., P. E. Ch’ng, and T. H. Lim. 2010. Determination of Some Physical Properties of Nutmeg (Myristica fragrans) Seeds. Research Journal of Applied Sciences, Engineering and Technology. 2(7): 669-672.

Li, T., X.-y. Qu, Q.-a. Zhang, and Z.-Z. Wang. 2012. Ultrasound-assisted Extraction And Profile Characteristics Of Seed Oil From Isatis indigotica Fort. Industrial Crops and Products. 35(1): 98-104.

Hu, A. J., Q. Q. Feng, J. Zheng, X. H. Hu, C. Wu, and C.-Y. Liu. 2012. Kinetic Model and Technology of Ultrasound Extraction of Safflower Seed Oil. Journal of Food Process Engineering. 35(2): 278-294.

Rodríguez-Solana, R., J. M. Salgado, J. M. Domínguez, and S. Cortés-Diéguez. 2014. Characterization Of Fennel Extracts And Quantification Of Estragole: Optimization And Comparison Of Accelerated Solvent Extraction And Soxhlet Techniques. Industrial Crops and Products. 52(0): 528-536.

Murali, H. S., M. S. Mohan, K. S. Mania, and R. Sankaran. 1993. Polar and Nonpolar Lipids and Their Fatty Acid Composition of a Few Fusarium Species. Journal of the American Oil Chemists' Society. 70(10): 1039-1041.

Crane, S., G. Aurore, H. Joseph, Z. Mouloungui, and P. Bourgeois. 2005. Composition Of Fatty Acids Triacylglycerols And Unsaponifiable Matter In Calophyllum Calaba L. Oil From Guadeloupe. Phytochemistry. 66(15): 1825-1831.

Ajayi, I. A., R. A. Oderinde, V. O. Taiwo, and E. O. Agbedana. 2008. Short-Term Toxicological Evaluation Of Terminalia catappa, Pentaclethra macrophylla and Calophyllum inophyllum Seed Oils In Rats. Food Chemistry. 106(2): 458-465.

Zhao, S., K.-C. Kwok, and H. Liang. 2007. Investigation On Ultrasound Assisted Extraction Of Saikosaponins From Radix Bupleuri. Separation and Purification Technology. 55(0): 307-312.

Tian, Y., Z. Xu, B. Zheng, and Y.M. Lo. 2013. Optimization Of Ultrasonic-Assisted Extraction Of Pomegranate (Punica granatum L.) seed oil. Ultrasonics Sonochemistry. 20(0): 202-208.

Zou, Y., C. Xie, G. Fan, Z. Gu, and Y. Han. 2010. Optimization Of Ultrasound-Assisted Extraction Of Melanin From Auricularia Auricula Fruit Bodies. Innovative Food Science & Emerging Technologies. 11(4): 611-615.

Dong, J., Y. Liu, Z. Liang, and W. Wang. 2010. Investigation On Ultrasound-Assisted Extraction Of Salvianolic Acid B From Salvia Miltiorrhiza Root. Ultrasonics Sonochemistry. 17(0): 61-65.

Maran, J. P., B. Priya, and C. V. Nivetha. 2015. Optimization Of Ultrasound-Assisted Extraction Of Natural Pigments From Bougainvillea Glabra Flowers. Industrial Crops and Products. 63(0): 182-189.

Xie, P.-j., L.-x. Huang, C.-h. Zhang, F. You, and Y.-l. Zhang. 2015. Reduced Pressure Extraction Of Oleuropein Fromolive Leaves (Olea Europaea L.) With Ultrasound Assistance. Food and Bioproducts Processing. 93(0): 29-38.

Wu, H., J. Zhu, W. Diao, and C. Wang. 2014. Ultrasound-Assisted Enzymatic Extraction And Antioxidant Activity Ofpolysaccharides From Pumpkin (Cucurbita Moschata). Carbohydrate Polymers. 113(0): 314-324.

Ordóñez-Santos, L. E., L. X. Pinzón-Zarate, and L. O. González-Salcedo. 2015. Optimization Of Ultrasonic-Assisted Extraction Of Total Carotenoids From Peach Palm Fruit (Bactris Gasipaes) By-Products With Sunflower Oil Using Response Surface Methodology. Ultrasonics Sonochemistry. http://dx.doi.org/10.1016/j.ultsonch.2015.04.010.

Zou, T.-B., M. Wang, R.-Y. Gan, and W.-H. Ling. 2011. Optimization of Ultrasound-Assisted Extraction of Anthocyanins from Mulberry, Using Response Surface Methodology. International Journal of Molecular Sciences. 12(0): 3006-3017.

Wei, M.-C. and Y.-C. Yang. 2014. Extraction Characteristics And Kinetic Studies Of Oleanolic And Ursolic Acids From Hedyotis Diffusa Under Ultrasound-Assisted Extraction Conditions. Separation and Purification Technology. 130(0): 182-192.

Ramandi, N. F., A. Ghassempour, N. M. Najafi, and E. Ghasemi. 2012. Optimization Of Ultrasonic Assisted Extraction Of Fatty Acids From Borago Officinalis L. Flower By Central Composite Design. Arabian Journal of Chemistry. http://dx.doi.org/10.1016/j.arabjc.2012.06.009.

Feng, S., Z. Luo, B. Tao, and C. Chen. 2015. Ultrasonic-Assisted Extraction And Purification Of Phenolic Compounds From Sugarcane (Saccharum ofï¬cinarum L.) rinds. LWT - Food Science and Technology. 60(2): 970-976.

Lou, Z., H. Wang, M. Zhang, and Z. Wang. 2010. Improved Extraction Of Oil From Chickpea Under Ultrasound In A Dynamic System. Journal of Food Engineering. 98(1): 13-18.

Xu, Y., L. Zhang, Y. Bailina, Z. Ge, T. Ding, X. Ye, and D. Liu. 2014. Effects Of Ultrasound And/Or Heating On The Extraction Of Pectin From Grapefruit Peel. Journal of Food Engineering. 126(0): 72-81.

Zhang, Q.-A., Z.-Q. Zhang, X.-F. Yue, X.-H. Fan, T. Li, and S.-F. Chen. 2009. Response Surface Optimization Of Ultrasound-Assisted Oil Extraction From Autoclaved Almond Powder. Food Chemistry. 116(0): 513–518.

Chao, Z., Y. Ri-fu, and Q. Tai-qiu. 2013. Ultrasound-Enhanced Subcritical Water Extraction Of Polysaccharides from Lycium barbarum L. Separation and Purification Technology. 120(0): 141-147.

Zhao, Z.-Y., Q. Zhang, Y.-F. Li, L.-L. Dong, and S.-L. Liu. 2015. Optimization Of Ultrasound Extraction Of Alisma Orientalis Polysaccharides By Response Surface Methodology And Their Antioxidant Activities. Carbohydrate Polymers. 119(0): 101-109.






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