STAGE-DEPENDENT EFFECTS OF Quassia borneensis NOOT. (SIMAROUBACEAE) CHLOROFORM EXTRACTS ON ERYTHROCYTES INFECTED WITH CHLOROQUINE-RESISTANT Plasmodium berghei NK65
DOI:
https://doi.org/10.11113/jt.v77.5999Keywords:
Q. borneensis, antimalarial, P. berghei, intraerythrocytesAbstract
This study was conducted to screen the antiplasmodial activity of chloroform extracts of Quassia borneensis against different intraerythrocytic cycle of chloroquine-resistant Plasmodium berghei NK65. The antiplasmodial activity was measured using SYBR green I assay for determining the concentration that inhibited 50% (IC50) of the Plasmodium’s activity after 24 hours of treatment (0.0001-100 mg mL-1). All of the extracts showed potent activity against all of the cycles. The trophozoite and schizont stages were considerably more sensitive to the extracts than the ring-stages parasites. The values were compared to the controls treated with chloroquine and artemisinin. Hence, the chloroform extracts of roots and bark of Q. borneensis demonstrated potent activities towards each life cycle of chloroquine-resistant P. berghei and has a potential to be developed as an antimalarial agent.References
Murray, C.J., Rosenfeld, L.C., Lim, S.S., Andrew, K.G., Foreman, K.J., Haring, D, Fullman, N., Naghavi, M., Lozano, R. and Lopez, A.D. 2012. Global Malaria Mortality Between 1980 and 2010: A Systematic Analysis. Lancet. 379: 413-431.
Health Facts. 2000. Department of Public Health, Ministry of Health, Malaysia. [Online]. From: http://dph.gov.my/Division/dcd/fakta/MSIA%20Facts.htm/. [Acessed on 3 January 2015].
Bertani, S.E., Houel, D., Stien, L., Chevolot, V., Jullian, G., Garavito, G., Bourdy, and Deharo, E. 2006. Simalikalactone D Is Responsible for The Antimalarial Properties of An Amazonian Traditional Remedy Made With Quassia amara L. (Simaroubaceae). Journal of Ethnopharmacology. 108: 155–157.
Chan, K.L., Choo, C.Y., Abdullah, N.R. and Ismail, Z. 2004. Antiplasmodial Studies of Eurycoma longifolia Jack Using The Lactate Dehydrogenase Assay of Plasmodium falciparum. Journal of Ethnopharmacology. 92: 223–227.
Apers, S., Cimanga, K., Berghe, D.V., Meenen, E.V., Longanga, A.O., Foriers, A., Vlietinck, A. and Peters, L. 2002. Antiviral Activity of Simalikalactone D, A Quassinoid from Quassia africana. Planta Med. 68: 20-24.
Gillin, F.D., Reiner, D.S. and Suffness, M. 1982. Bruceantin, A Potent Amoebicide From A Plant, Brucea antidvsenterica. Antimicrob Antimicrobial Agents and Chemotherapy. 22: 342-345.
Keene, A. T., Harris, A., Phillipson, J.D. and Warhurst, D.C. 1986. In Vitro Amoebicidal Testing of Natural Products; Part I. Methodology. Planta Medica. 52: 278-285.
Polonsky, J. 1983. Chemistry and Biological Activity of the Quassinoids. In: Chemistry and Chemical Taxonomy of The Rutales. London: Academic Press. 247-266.
Hall, I.H., Lee, K.H., Imakura, Y., Okano, M. and Johnson, A. 1983. Antiinflammatory Agents III. Structure-Activity Relationships of Brusatol and Related Quassinoids. Journal of Pharmaceutical Sciences. 12: 1282-1284.
Stiffness, M. and Douros, J.D. 1979. Drugs of plant origin. In: Method in Cancer Research. New York: Academic Press. 73-126.
Pierre, A., Robert-Gero, M., Tempete, C. and Polonsky, J. 1980. Structural Requirements of Quassinoids for The Inhibition of Cell Transformation. Biochemical & Biophysical Research Communications. 93: 675-686.
Guru, P.Y., Warhurst, D.C., Harris, A. and Phillipson, J.D. 1983. Antimalarial Activity of Bruceantin In Vitro. Annals of Tropical Medicine and Parasitology. 77: 433-435.
Fandeur, T., Moretti, C. and Polonsky, J. 1985. In Vitro and In Vivo Assessment of The Antimalarial Activity of Permeability of Individual Quassinoids or from Inherent Geolide. Planta Medica. 51: 20-23.
Nuddin, J.A., Indim@Hindin, D., Tajudin, M.H.A., Rahman, N.A.A., Rahman, S.S.A., Ahmad, L.W.Z., Kulip, J., Ghazali, A.R., Adam, A. and Hamzah, A.S. 2010. Simaroubaceae of Sabah: The Next Tongkat Ali? Conservation of Biodiversity in Trus Madi Forest Reserve Workshop, pg. 133-145.
Ghazali, A.R., Nuddin, J.A., Sabireen, S.N., Razali, W.M.W. and Ishak, S.A. 2013. Ex vivo Antiplasmodial Activity of Quassia borneensis Noot. (Simaroubaceae) Extracts Against Mice Erythrocytes Infected by Plasmodium berghei NK65. International Journal of Undergraduates Studies. 2(3): 24-31.
Kirby, G.C., O’Neill, M.J., Phillipson, J.D. and Warhurst, D.C. 1989. In vitro Studies on The Mode of Action of Quassinoids with Activity Against Chloroquine-Resistant Plasmodium falciparum. Biochemical Pharmacology. 38(24): 4367-4374.
Awang, N., Jumat, H., Ishak, S.A., & Kamaludin, N.F. 2014. Evaluation of The Ex vivo Antimalarial Activity of Organotin (IV) Ethylphenyldithiocarbamate on Erythrocytes Infected with Plasmodium berghei Nk 65. Pakistan Journal of Biological Sciences. 17(6): 836-842.
Sriprawat, K., Kaewpongsri, S., Suwanarusk, R., Leimanis, M.L., Lek-Uthai, U., Phyo, A.P., Snounou, G., Russell, B., Renia, L., and Nosten, F. 2009. Effective and Cheap Removal of Leukocytes and Platelets from Plasmodium vivax Infected Blood. Malaria Journal. 8: 115.
Lambros, C., and Vanderberg, J.P. 1979. Synchronization of Plasmodium falciparum Erythrocytic Stages in Culture. Journal of Parasitology. 65: 418–420.
Smilkstein, M., Sriwilaijaroen, N., Kelly, J.X., Wilairat, P. and Riscoel, M. 2004. Simple and Inexpensive Fluorescence-Based Technique for High-Throughput Antimalarial Drug Screening. Antimicrobial Agents and Chemotherapy. 48(5): 1803-1806.
Pink, R., Hudson, A., Mouries, M.A. and Bending, M. 2005. Opportunities and Challenges in Antiparasitic Drug Discovery. Nature Reviews Drugs Discovery. 4: 727-740.
Jonville, M.C., Kodja, H., Humeau, L., Fourne, J., De Mol, P., Cao, M., Angenot, L. and Frederich, M. 2008. Screening of Medicinal Plants from Reunion Island for Antimalarial and Cytotoxic Activity. Journal Ethnopharmacology. 120: 382-386.
Wright, C.W., 2004. Pharmacological Properties of The Active Constituents of Some Traditional Herbal Antimalarials. In: Traditional Medicinal Plants and Malaria. London : CRC Press. 265-291.
Yayon, A., Waa, J.A.V., Yayon, M., Geary, T.G., and Jensen, J.B. 1983. Stage-Dependent Effects of Chloroquine on Plasmodium falciparum In vitro. Journal of Protozoology 30(4): 642-647.
Cachet, N., Hoakwie, F., Bertani, S., Bourdy, G., Deharo, E., Stien, D., Houel, E., Gornitzka, H., Fillaux, J., Chevalley, S., Valentin, A. and Jullian, V. 2009. Antimalarial Activity of Simalikalactone E, A New Quassinoid from Quassia amara L. (Simaroubaceae). Antimicrobial Agents and Chemotherapy. 53: 4393-4398.
Bertani, S., Houel, E., Jullian, V., Bourdy, G., Valentin, A., Stien, D. and Deharo, E. 2012. New Findings on Simalikalactone D, An Antimalarial Compound from Quassia amara L. (Simaroubaceae). Experimental of Parasitology. 130: 341.
Landau, I., Lepers, J.P, Ringwald, P., Rabarison, P., Ginsburg, H., and Chabaud, A. 1992. Chronotherapy of Malaria: Improved Efficacy of Timed Chloroquine Treatment of Patients with Plasmodium falciparum Infections. Transactions of the Royal Society of Tropical Medicine and Hygiene. 86: 374–375.
Enosse, S., Butcher, G.A., Margos, G., Mendoza, J., Sinden, R.E., and Hogh, B. 2000. The Mosquito Transmission of Malaria: The Effects of Atovaquone-Proguanil (Malarone) and Chloroquine. Transactions of the Royal Society of Tropical Medicine and Hygiene. 94: 77-82.
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