AN in vitro STUDY OF WOUND HEALING ACTIVITY OF Ficus deltoidea LEAF EXTRACT
DOI:
https://doi.org/10.11113/jt.v77.6008Keywords:
Ficus deltoidea, scratch assay, fibroblast, cell proliferative, wound healingAbstract
Ficus deltoidea (Mas Cotek) is one of the popular herbs that has been used traditionally to alleviate illnesses. The present study aimed to investigate the in vitro wound healing activity of  F. deltoidea leaf extract on skin cell. Cell proliferative and migration assay were done on the Human Skin Fibroblast cell (HSF 1184) which were treated with different concentrations of F. deltoidea leaf extract. The data of wound closure were collected at time intervals of 0, 6, 12 and 24 hours and analyzed using ImageJ™ software. MTT assay revealed that the F. deltoidea leaf extract could induce cell proliferation at a dose dependent manner. F. deltoidea leaf extract significantly accelerated the wound closure process in comparison to cells treated with ascorbic acid and untreated cells in scratch assay. Following 24 hours of incubation, cells treated with 50 mg/mL of F. deltoidea leaf extract showed remarkable proliferative and wound closure effect with 143.67% and 5.96%, respectively as compared to other concentrations. Collectively, these findings suggested that F. deltoidea leaf extract possesses wound healing potential and may be useful for the development of efficient wound healing drug.
References
Zakaria, Z. A., Hussain, M. K., Mohamad, A. S., Abdullah, F. C. and Sulaiman, M. R. 2012. Anti-inflammatory Activity of the Aqueous Extract of Ficus deltoidea. Biological Research for Nursing. 14(1): 90-97.
Wahid, S., Mahmud, T. M. M., Maziah, M., Yahya, A. and Rahim, M. Ab. 2010. Total Phenolics Content and Antioxidant Activity of Hot Water Extract from Dried Ficus deltoidea Leaves. Journal of Agriculture and Food Science. 38(1): 115-122.
Hamidun, B., Noriha, M. A., Siti Noraini, B., Syarul Nataqain, B. and Normah, M. N. 2014. Ficus deltoidea Jack: A Review on Its Phytochemical and Pharmalogical Importance. http://dx.doi.org/10.1155/2014/902734. [4 August 2014].
Justine, I. B. 2008. Total Phenolic Content and Scavenging Activity of Water and Methanol Extracts from the Stem of Mas Cotek (Ficus deltoidea). Faculty of Applied Science, University Teknologi Mara. 2-13.
Hasham, R., Hyun-Kyung, C., Sarmidi, M. R. and Chane-Seo, P. 2013. Protective Effects of a Ficus deltoidea (Mas Cotek) Extract against UVB-Induced Photoageing in Skin Cells. Biotechnology and Bioprocess Engineering. 18: 185-193.
Mansor, H. and Mahmood, M. 2009. Non Enzymatic and Enzymatic Antioxidant Activities in Aqueous Extract of Different Ficus deltoidea Accesions. Journal of Medicinal Plants Research. 3(3): 120-131.
Abdullah, Z., Hussain, K., Zhari, I., Rasadah, M. A., Jamaludin, F. and Sahdan R. 2009. Evaluation of Extracts of Leaf of Three Ficus deltoidea Varieties for Antioxidant Activities and Secondary Metabolites. Pharmacognosy Research. 1: 216-223.
Walter, M. N. M., Wright, K. T., Fuller, H. R., MacNeil, S. and Johnson, W. E. B. 2010. Mesenchymal Stem Cell-conditioned Medium Accelerates Skin Wound Healing: An In Vitro Study of Fibroblast and Keratinocyte Scratch Assays. Experimental Cell Research. 316: 1271-1281.
Ramamurthy, S., Kumarappan, C., Dharmalingam, S. R. and Sangeh, J. K. 2014. Phytochemical, Pharmacological and Toxicological Properties of Ficus deltoidea: A Review of a Recent Research. Annual Research & Review in Biology. 4(14): 2357-2371.
Abdulla, M. A., Ahmed, K. A-A., Abu-Luhoom, F. M. and Muhanid, M. 2010. Role of Ficusn deltoidea Extract in the Enhancement of Wound Healing in Experimental Rats. Biomedical Research. 21(3): 241-245.
Blois, M. S. 1958. Antioxidant Determinations by the Use of a Stable Free Radical. Nature. 29: 1199-1200.
Mosmann, T. 1983. Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays. Journal of Immunology Methods. 65: 55-63.
Sirisha, N., Sreenivasulu, M., Sangeeta, K. and MadhusudhanaChetty, C. 2010. Antioxidant Properties of Ficus Species-A Review. International Journal of PharmTech Research. 2(4): 2174-2182.
Nor Azurah, M. A., Chua, L. S., Fadzilah Adibah, A. M. and Mohamad Roji, S. 2011. Cytotoxicity of Aqueous and Ethanolic Extracts of Ficus deltoidea on Human Ovarian Carcinoma Cell Line. British Journal of Medicine & Medical Research. 1(4): 397-409.
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