CYTOTOXIC EFFECT OF HEPCIDIN (TH1-5) ON HUMAN BREAST CANCER CELL LINE (MCF7)

Authors

  • Mohammed Al-kassim Hassan Faculty of Pharmaceutical Sciences, Bayero University, Kano
  • Wan-Atirah Azemin Faculty of Bioresources and Food Industry, Universiti Sultan Zainal Abidin, Tembila Campus, 22200 Besut, Terengganu, Malaysia
  • Saravanan Dharmaraj Faculty of Medicine, Universiti Sultan Zainal Abidin, Medical Campus, Jalan Sultan Mahmud, 20400 Kuala Terengganu, Terengganu, Malaysia
  • Khamsah Suryati Mohd Agriculture Production and Food Innovation Research Institute, AGROPOLIS, UniSZA, Malaysia

DOI:

https://doi.org/10.11113/jt.v77.6009

Keywords:

Hepcidin (TH1-5), antimicrobial peptide, cytotoxicity, MCF7

Abstract

Hepcidin (TH1-5) is a cysteine-rich antimicrobial peptide originally isolated from the freshwater fish Oreochromis mossambicus. A synthesized form of the peptide has been reported to exhibit cytotoxic activity against few human cancer cell lines. This study investigated the potential cytotoxicity of the peptide against human breast cancer cell line and normal mouse embryonic fibroblast cell line (NIH/3T3) using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Morphological changes by acridine orange and propidium iodide (AO/PI) double staining were also studied to determine apoptotic evidences. Hepcidin (TH1-5) showed cytotoxic activity against MCF7 with an IC50 of 20 mg/mL but no significant effect against NIH/3T3. This outcome indicates hepcidin (TH1-5) to be a promising cytotoxic peptide that warrants further studies as a potential anticancer agent for breast cancer therapy.

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Published

2015-10-28

How to Cite

CYTOTOXIC EFFECT OF HEPCIDIN (TH1-5) ON HUMAN BREAST CANCER CELL LINE (MCF7). (2015). Jurnal Teknologi, 77(3). https://doi.org/10.11113/jt.v77.6009