CYTOTOXIC EFFECT OF HEPCIDIN (TH1-5) ON HUMAN BREAST CANCER CELL LINE (MCF7)
DOI:
https://doi.org/10.11113/jt.v77.6009Keywords:
Hepcidin (TH1-5), antimicrobial peptide, cytotoxicity, MCF7Abstract
Hepcidin (TH1-5) is a cysteine-rich antimicrobial peptide originally isolated from the freshwater fish Oreochromis mossambicus. A synthesized form of the peptide has been reported to exhibit cytotoxic activity against few human cancer cell lines. This study investigated the potential cytotoxicity of the peptide against human breast cancer cell line and normal mouse embryonic fibroblast cell line (NIH/3T3) using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Morphological changes by acridine orange and propidium iodide (AO/PI) double staining were also studied to determine apoptotic evidences. Hepcidin (TH1-5) showed cytotoxic activity against MCF7 with an IC50 of 20 mg/mL but no significant effect against NIH/3T3. This outcome indicates hepcidin (TH1-5) to be a promising cytotoxic peptide that warrants further studies as a potential anticancer agent for breast cancer therapy.
References
Leuschner, C. and Hansel, W. 2004. Membrane Disrupting Lytic Peptides for Cancer Treatments. Current Pharmaceutical Design. 10(19): 2299-2310.
Kamysz, W., Okrój, M. and Åukasiak, J. 2005. Novel Properties of Antimicrobial Peptides. Acta Biochimia Polinica. 50(2): 461-469.
Pushpanathan, M., Gunasekaran, P. and Rajendhran, J. 2013. Antimicrobial Peptides : Versatile Biological Properties. International Journal of Peptides. 1-15.
Najafian, L. and Babji, A. S. 2012. Peptides A Review of Fish-derived Antioxidant and Antimicrobial Peptides : Their Production , Assessment, and Applications. Peptides. 33(1): 178-185.
Hancock, R. E. W. and Patrzykat, A. 2002. Clinical Development of Cationic Antimicrobial Peptides: From Natural to Novel Antibiotics. Current Drug Targets Infectious Disorders. 2(1): 79-83.
Shi, J. and Camus, A. C. 2006. Hepcidins in Amphibians and Fishes: Antimicrobial Peptides or Iron-regulatory Hormones? Developmental & Comparative Immunology. 30: 746-755.
Ganz, T. 2003. Hepcidin, a Key Regulator Of Iron Metabolism and Mediator af Anemia of Inflammation. Blood. 102(3): 783-788.
Douglas, S. E., Gallant, J. W., Liebscher, R. S., Dacanay, A. and Tsoi S.C. 2003. Identification and Expression Analysis of Hepcidin-like Antimicrobial Peptides in Bony Fish. Developmental & Comparative Immunology. 27(6-7): 589-601.
Hunter, H. N., Bruce, F. D., Ganz, T. and Vogel, H. J. 2002. The Solution Structure of Human Hepcidin, a Peptide Hormone with Antimicrobial Activity that is Involved in Iron Uptake and Hereditary Hemochromatosis. Journal of Biological Chemistry. 277(40): 37597-37603.
Brogden, K. A. 2005. Antimicrobial Peptides: Pore Formers or Metabolic Inhibitors in Bacteria? Nature Reviews Microbiology. 3(3): 238-250.
Huang, P. H., Chen, J. Y. and Kuo, C. M. 2007. Three Different Hepcidins from Tilapia, Oreochromis mossambicus: Analysis of their Expressions and Biological Functions. Molecular Immunology. 44(8): 1922-1934.
Chang, W-T., Pan, C-Y., Rajanbabu, V., Cheng, C-W. and Chen, J-Y. 2011. Tilapia (Oreochromis mossambicus) Antimicrobial Peptide, Hepcidin 1-5, Shows Antitumor Activity in Cancer Cells. Peptides. 32(2): 342-52.
Chen, J., Lin, W-J. and Lin, T-L. 2009. A Fish Antimicrobial Peptide, Tilapia Hepcidin TH2-3, Shows Potent Antitumor Activity Against Human Fibrosarcoma Cells. Peptides. 30(9): 1636-42.
Boyle, P. and Lewin, B. World Cancer Report 2008.
Ebrahim, K., Shirazi, F.H., Vatanpour, H., Kobarfard, F. and Rabiei, H. 2014. Anticancer Activity of Cobra Venom Polypeptide, Cytotoxin-II, Against Human Breast Adenocarcinoma Cell Line ( MCF-7 ) via the Induction of Apoptosis. Journal of Breast Cancer. 17(4): 314-322.
Mathers, C. D. and Loncar, D. 2006. Projections of Global Mortality and Burden of Disease from 2002 to 2030. PLoS Medicine. 3(11): 2011-2030.
Patel, N. M., Nozaki, S., Shortle, N.H., Bhat-Nakshatri, P., Newton, T.R., Rice, S., Gelfanov, V., Boswell, S.H., Goulet Jr, R.J., Sledge Jr, G.W., and Nakshatri, H. 2000. Paclitaxel Sensitivity of Breast Cancer Cells with Constitutively Active NF-kappaBis Enhanced by IkappaBalpha Super-repressor and Parthenolide. Oncogene. 19: 4159-4169.
Tajudin, T. J. S. A., Mat, N., Siti-Aishah, A.B., Yusran, A. A. M., Alwi, A. and Ali, A. M. 2012. Cytotoxicity, Antiproliferative Effects, and Apoptosis Induction of Methanolic Extract of Cynometra cauliflora Linn. Whole Fruit on Human Promyelocytic Leukemia HL-60 cells. Evidence-based Complementary and Alternative Medicine. doi.org/10.1155/2012/127373.
Mosmann, T. 1983. Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays. Journal of Immunological Methods. 1983. 65(1-2): 55-63.
Muhammad, N. N., Abdul, A. B., Sukari, M. A., Abdelwahab, S. I., Eid, E. E. M. and Mohan, S. et al. 2013. Inclusion Complex of Zerumbone with Hydroxypropyl-Î’-Cyclodextrin Induces Apoptosis in Liver Hepatocellular HepG2 Cells via Caspase 8/Bid Cleavage Switch and Modulating Bcl2/Bax Ratio. Evidence-based Complementary and Alternative Medicine. doi:org/10.1155/2013/816632.
Li, Y., Huang, W., Huang, S., Du, J. and Huang, C. 2012. Screening of Anti-cancer Agent Using Zebrafish: Comparison with the MTT Assay. Biochemical and Biophysical Research Communications. 422(1): 85-90.
Chan, K. M., Rajab, N. F., Ishak, M. H. A., Ali, A. M., Yusoff, K., Din L.B. et al. 2006. Goniothalamin Induces Apoptosis in Vascular Smooth Muscle Cells. Chemico-Biological Interaction. 159(2): 129-40.
Downloads
Published
Issue
Section
License
Copyright of articles that appear in Jurnal Teknologi belongs exclusively to Penerbit Universiti Teknologi Malaysia (Penerbit UTM Press). This copyright covers the rights to reproduce the article, including reprints, electronic reproductions, or any other reproductions of similar nature.
