DNA BARCODING OF CAGED PANGASIIDS IN PAHANG RIVER MALAYSIA
Keywords:Pangasidae, Pahang River, endangered fishes, COI
AbstractThe domestication aquaculture selection in farm environment can cause changes in phenotype and genotype of farmed fishes, which may later influence the demographic structure of wild populations if they accidentally escaped from cages. This is an alarming situation for conservation of native species of Pangasius sp. in Pahang River. Hence, the present study was aimed to investigate genetic variation among the fishes collected from different cages from Pahang River. We adopted conventional taxonomical approach to identify species and cross-examined using universal barcode gene Cytochrome Oxydase Subunit 1 (COX1) gene. Samples were collected from 6 commercial cages from Pahang River. Haplotype and genetic diversity among the fishes from different cages were determined. Results from Neighbor Joining tree showed that most of the samples were identified to be Pangasianodon hypothalamus despite having different morphometric character. This study also revealed that most of the caged Pangasius cultured in Pahang River are exotic and non-native to Malaysia. Thus, a continuous monitoring through studies on genetic variation of Pangasius sp. is an essential need for the sustainable development of this endangered fishes in Pahang River.
Abdul Muneer PM, G. A. 2011. Genetic Variation and Phylogenetic Relationship between Two Species of Yellow Catfish, Horabagrus brachysoma and H.nigricollaris (Teleostei: Horabagridae) based on RAPD and Microsatellite Markers. Molecular Biology Reports. 38(4): 2225-2232.
Mukherjee, T. K. 2001. Genetics for Improvement of Fish in Malaysia. Fish Genetics Research in Member Countries and Institution of the International Network on Genetics in Aquaculture. 65-70.
Tanya, C., & Kumar, R. (Published Online October, 2010). Advances in Bioscience and Biotechnology. Retrieved from (http://www.SciRP.org/journal/abb/)FAO. (2010, January 14) Cultured Aquatic Species Information Programme Pangasius Hypothalmus, Cultured Aquatic Species information Programme.
FAO Fisheries and Aquaculture Department [online]. Rome: http://www.fao.org/fishery/culturedspecies/ PangasiusHypothalmus/enRetrieved July 14, 2014, from Text by Griffiths, D., Van Khanh, P., Trong, T.Q.
Thalathiah. 1988. Induced Spawning Techniques Practiced at Batu Berendam, Melaka, Malaysia. Aquaculture. 23-33.
Mohd Zafri, H., Christianus, A., Siraj, S. S., & Daud, K. S. 2005. The Patinof Pahang River. Fishmail. 13(4, January-March 2005): 6-7.
Ivanova, N. V. 2007. Universal Primer Cocktails for Fish DNA Barcoding. Molecular Ecology Notes. 7: 544-548.
Tamura, K., & Nei, M. 1993. Estimation of the Number of Nucleotide Substitutions in the Control Region of Mitochondrial DNA in Humans and Chimpanzees. Molecular Biology and Evolution. 10: 512-526.
Haslawati, B. 2004. Mengenali Ikan Patin. Retrieved September, 2014, from Forest Research Institute: http://www.pppat.gov.my/fish/kenalipatin.pdf.
Abdul Muneer PM, G. A. 2011. Genetic Variation and Phylogenetic Relationship Beteen Two Species of Yellow Catfish, Horabagrus brachysoma and H.nigricollaris (Teleostei: Horabagridae) based on RAPD and Microsatellite Markers. Molecular Biology Reports. 38(4): 2225-2232.
Roberts, T., &Vidhayanon, C. 1991. Systematic Revision of the Asian Catfish family Pangasiidae, with Biological Observation and Description of Three New Species. Philad. Proc. Acad. Nat. Sci. 143: 97-143.
Pouyad, L., Teugels, G. G., & Legendre, M. 1999. Description of a New Pangasiid Catfish from South-East Asia (Siluriformes).Cybium. 23(3): 247-258.
Mohd, Z. H. 2007. Morphology and General Reproductive Stages of Pangasiusnasutus from Sg. Pahang in Maran District, Pahang, Malaysia. Master's Thesis (UPM). 139.
Anuar, H., MohdAzmi, Agus Putra, & A., S. 2011. Crossbreeding of Pangasianodo nhypothalmus( Sauvage,1878) and Pangasiusnasatus (Bleeker, 1863) and Their Larval Development. Journal of Sustainability Science and Management. 6(1): 28-35.
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