RANDOM AMPLIFICATION POLYMORPHIC DNA-PCR (RAPD) ANALYSIS OF Vibrio alginolyticus STRAINS ISOLATED FROM GREEN MUSSELS (Perna viridis) IN MARUDU BAY, SABAH

Authors

  • M. Munirah Institute of Marine Biotechnology, Universiti Malaysia Terengganu, Malaysia
  • Muhd Danish-Daniel School of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu, Malaysia
  • C. S. Vairappan Institute for Tropical Biology and Conservation, Universiti Malaysia Sabah, Malaysia
  • A. W. M. Effendy Institute of Marine Biotechnology, Universiti Malaysia Terengganu, Malaysia

DOI:

https://doi.org/10.11113/jt.v77.6738

Keywords:

Vibrio alginolyticus, green mussels (Perna viridis), random amplified polymorphic DNA (RAPD)

Abstract

Vibrio alginolyticus infection is common in aquatic species. The species has been isolated from infected green mussels (Perna viridis) cultivated at Marudu Bay, Sabah.  The infection of V. alginolyticus was prevalent since 2009 and caused more than 60% mortality of the mollusc. This study was conducted on V. alginolyticus isolated from clinically infected P. viridis at cultivation area in Marudu Bay from 2013. Twenty isolates were randomly selected and subjected to RAPD-PCR analysis using 20 sets of RAPD primers. Results showed that the most suitable primers were OPA 3, OPA 5, OPA 9, OPA 10 and OPA 12 where the significant bands ranging from 100-2500 bp. Genetic variability among the isolates as revealed by these primers indicated that RAPD-PCR method has good discriminative ability and can be used as a rapid typing method of differentiating V. alginolyticus strains isolated from infected green mussels for epidemiological investigation.

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Published

2015-12-13

How to Cite

Munirah, M., Danish-Daniel, M., Vairappan, C. S., & Effendy, A. W. M. (2015). RANDOM AMPLIFICATION POLYMORPHIC DNA-PCR (RAPD) ANALYSIS OF Vibrio alginolyticus STRAINS ISOLATED FROM GREEN MUSSELS (Perna viridis) IN MARUDU BAY, SABAH. Jurnal Teknologi, 77(25). https://doi.org/10.11113/jt.v77.6738