INVESTIGATION ON THE DIFFERENCE EXPRESSION OF TYPE IV COLLAGEN ï¡1(IV)- ï¡6(IV) CHAIN MRNA IN NORMAL FIBROBLAST AND IN SKIN CELL MALIGNANT MELANOMA

Authors

  • Mardhiah Mohammad Integrated Cellular and Molecular Biology Cluster (iMolec), Integrated Centre for Animal Care and Use, International Islamic University Malaysia, Indera Mahkota Campus, Jalan Sultan Ahmad Shah, 25200 Kuantan, Pahang, Malaysia
  • Afzan Mat Yusof Integrated Cellular and Molecular Biology Cluster (iMolec), Integrated Centre for Animal Care and Use, International Islamic University Malaysia, Indera Mahkota Campus, Jalan Sultan Ahmad Shah, 25200 Kuantan, Pahang, Malaysia
  • Syahirah Kaja Mohideen Department of Biomedical Science, Kulliyyah of Allied Health Sciences, International Islamic University of Malaysia Indera Mahkota Campus, Jalan Sultan Ahmad Shah, 25200 Kuantan, Pahang, Malaysia
  • Sharifah Norbaizura Syed Bahrom Department of Biomedical Science, Kulliyyah of Allied Health Sciences, International Islamic University of Malaysia Indera Mahkota Campus, Jalan Sultan Ahmad Shah, 25200 Kuantan, Pahang, Malaysia
  • Ridhwan Roshdi Department of Biomedical Science, Kulliyyah of Allied Health Sciences, International Islamic University of Malaysia Indera Mahkota Campus, Jalan Sultan Ahmad Shah, 25200 Kuantan, Pahang, Malaysia
  • Adibah Parman Department of Biomedical Science, Kulliyyah of Allied Health Sciences, International Islamic University of Malaysia Indera Mahkota Campus, Jalan Sultan Ahmad Shah, 25200 Kuantan, Pahang, Malaysia

DOI:

https://doi.org/10.11113/jt.v77.6754

Keywords:

Collagen, type IV collagen, melanoma cell lines, qPCR, gene expression

Abstract

Collagen IV is the major basement membrane protein that influences adhesion, proliferation, and migration of cells. The collagen composed of a network chains a1 to a6. The characterization of this collagen IV will correlates the relationship of collagen gene expression and cancer. This is important in order to provide more detailed understanding of the expression of collagen in tumor cells. . The aim of this study is to determine the a1 to a6 (IV) mRNA expression in the cell lines obtained from skin and melanoma cell. To investigate the mRNA expression, the RNA was extracted from the fibroblast and melanoma (A375) cell lines. The RNA was subjected to reverse transcription and then synthesized. The mRNA expression levels were measured using real time PCR with related to internal control, GAPDH. The study identified that a1, a2, a4, a5 and a6 of the a1-a6 (IV) were expressed in skin fibroblast. This corresponds to the a1a1a2 and a5a5a6 networks. However, in melanoma cell lines the collagen IV a2, a4, a5 and a6 mRNA was observed in low level compared to a1 and this suggested that the tumor has affected the expression of collagen and basement membrane of the cell.

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Published

2015-12-13

How to Cite

INVESTIGATION ON THE DIFFERENCE EXPRESSION OF TYPE IV COLLAGEN 1(IV)- 6(IV) CHAIN MRNA IN NORMAL FIBROBLAST AND IN SKIN CELL MALIGNANT MELANOMA. (2015). Jurnal Teknologi, 77(25). https://doi.org/10.11113/jt.v77.6754