INVESTIGATION ON THE DIFFERENCE EXPRESSION OF TYPE IV COLLAGEN ï¡1(IV)- ï¡6(IV) CHAIN MRNA IN NORMAL FIBROBLAST AND IN SKIN CELL MALIGNANT MELANOMA
DOI:
https://doi.org/10.11113/jt.v77.6754Keywords:
Collagen, type IV collagen, melanoma cell lines, qPCR, gene expressionAbstract
Collagen IV is the major basement membrane protein that influences adhesion, proliferation, and migration of cells. The collagen composed of a network chains a1 to a6. The characterization of this collagen IV will correlates the relationship of collagen gene expression and cancer. This is important in order to provide more detailed understanding of the expression of collagen in tumor cells. . The aim of this study is to determine the a1 to a6 (IV) mRNA expression in the cell lines obtained from skin and melanoma cell. To investigate the mRNA expression, the RNA was extracted from the fibroblast and melanoma (A375) cell lines. The RNA was subjected to reverse transcription and then synthesized. The mRNA expression levels were measured using real time PCR with related to internal control, GAPDH. The study identified that a1, a2, a4, a5 and a6 of the a1-a6 (IV) were expressed in skin fibroblast. This corresponds to the a1a1a2 and a5a5a6 networks. However, in melanoma cell lines the collagen IV a2, a4, a5 and a6 mRNA was observed in low level compared to a1 and this suggested that the tumor has affected the expression of collagen and basement membrane of the cell.
References
Maynes, R. (Ed.). 2012. Structure and Function of Collagen Types. Elsevier.
Monboisse, J. C., Oudart, J. B., Ramont, L., Brassart-Pasco, S., & Maquart, F. X. 2014. Matrikines from Basement Membrane Collagens: A New Anti-Cancer Strategy. Biochimica et Biophysica Acta (BBA)-General Subjects. 1840(8): 2589-2598.
Hudson, B. G., Reeders, S. T. and Tryggvason, K. 1993. Type IV Collagen: Structure, Gene Organization, and Role in Human Diseases- Molecular Basis of Goodpasture and Alpert Syndromes and Diffuse Leiomyomatosis. J Biol Chem. 268(35): 26033-6.
Timpl, R. and Brown, J. C. 1996. Supramolecular Assembly of Basement Membranes. Bioessays. 18(2): 123-32.
Peissel, B., Gong, L., KaJJuri, R., Kashtan, C., Rennke, H. G., Ga(lo, G, R., Yoshioka, K, Sun, M. J., Hudson, B. G., NeiJson, E. G. and et al. 1995. Comparative Dlstribution of the Alpha 1(JV), Alpha 5(IV), and Alpha 6(JV) Collagen, Chains in Normaj Human Adult and Fetal Tissues and In Kidneys from X- Linked Alport Syndrome Patlents. J Cjin Invest. 96(4): 1948-57.
Harvey, S. J., Zheng, K., Sado, Y., Naito, I., Ninomiya, Y., Jacobs, R. M., Hudson, B. G., Thorner, P. S. 1998. Role of Distinct Type IV Collagen Networks in Glomerular Development and Function. Kidney Int. 54(6): 1857-66.
Timpl, R., Wiedemann, H., van Delden, V., Furthmayr, H., and Kuhn, K. 1981. A Network Model for the Organization of Type IV Collagen Molecules in Basement Membranes. Eur J Biochem. 120(2): 203-11.
Ninomiya, Y., Kagawa, M., lyama, K., Naito, I., Kishiro, Y., Sayer, J. M., Sugimoto, M., Oohashi, T. and Sado, Y. 1995. Differential Expression of Two Basement Membrane Collagen Genes, COL4A6 and COL4A5, Demonstrated by Immunofluorescence Staining Using Peptide-Speciftc Monoclonal Antibodies. J Cell Biol. 130(5): 1219-29.
Adams, J. C. and Watt, F. M. 1993. Regulation of Development and Differentia- Tion by the Extracellular Matrix. Development. 117: 1183-1198
Furcht, L. T., Skubitz, A. P. N. and Fields, G. B. 1994. Tumor Cell Invasion, Matrix Metalloproteinases, and the Dogma. Lab. Invest. 70: 781-783
Rowe & Weiss. 2008. Rowe, R. G., & Weiss, S. J. 2008. Breaching the Basement Membrane: Who, When and How?. Trends in Cell Biology. 18(11): 560-574.
Luparello, C. 2013. Aspect of Collagen Changes in Breast Cancer. Carcinogenesis & Mutagenesis. S13: 007.
Diaconescu, D. 2014. Evaluation of Type IV Collagen Expression in Squamous Cell Carcinoma of the Larynx. ulletin of the Transilvania University of Brasov, Seriels VI: Medical Sciences. 7(1).
Saftlas, A., Hoover, R., Brinton, L., Szklo, M. & Wolfe, J. 1998. Mammographic Densities as Indicators of Breast-cancer Risk. Am J Epidemiol. 128(4): 914.
Liang, Y., Diehn, M., Bollen, A. W., Israel, M. A., & Gupta, N. 2008. Type I Collagen is Overexpressed in Medulloblastoma as a Component of Tumor Microenvironment. Journal of Neuro-oncology. 86(2): 133-141.
Ohlund, D., Franklin, O., Lundberg, E., Lundin, C., & Sund, M. 2013. Type IV Collagen Stimulates Pancreatic Cancer Cell Proliferation, Migration, and Inhibits Apoptosis Through an Autocrine Loop. BMC Cancer. 13(1): 154.
Skovbjerg, H., Anthonsen, D., Lothe, I. M., Tveit, K. M., Kure, E. H., & Vogel, L. K. 2009. Collagen mRNA Levels Changes During Colorectal Cancer Carcinogenesis. BMC Cancer. 9(1): 136.
Mario, A. S., Surabhi, D. G., Amanda, J. R., & Hidayatullah, G. M. 2012. Biochemical Role of the Collagen-Rich Tumour Microenvironment in Pancreatic Cancer Progression. Biochemical Journal. 441(2): 541-552.
Dangi-Garimella, S., Krantz, S. B., Barron, M. R., Shields, M. A., Heiferman, M. J., Grippo, P.J & Munshi, H. G. 2011. Three-dimensional Collagen I Promotes Gemcitabine Resistance in Pancreatic Cancer Through MT1-Mmpmediated Expression of HMGA2. Cancer Research. 71(3): 1019-1028.
Reed, G. F., Lynn, F., & Meade, B. D. 2002. Use of coefficient Ofvariation in Assessing Variability of Quantitative Assays. Clinical & Diagnostic Laboratory Immunology. 9: 1235-1239.
Murray, L. S., Lu, Y., Taggart, A., Van Regemorter, N., Vilain, C., Abramowicz, M., Van Agtmael, T. 2014. Chemical Chaperone Treatment Reduces Intracellular Accumulation of Mutant Collagen IV and Ameliorates the Cellular Phenotype of a COL4A2 Mutation that Causes Haemorrhagic Stroke. Human Molecular Genetics. 23(2): 283-92.
Karachi, S. and Ninomiya, Y. 2005. Myocardial Infarction and Cardiac Fibrogenesis. In Razzaque, M. S. Fibrogenesis: Cellular and Molecular Basis. (83). U.S.A: Kluwer Academic/Plenum Publisher
Debbie, S. K., Labelle-Dumais, C. and Gould, D. B. 2012. COL4A1 and COL4A2 Mutations and Disease: Insights into Pathogenic Mechanisms and Potential Therapeutic Targets. Human Molecular Genetics. 21(1): 97-110.
Olsen, D. R., Mon-Li C. and Uitto, J. 1988. Expression of Basement Membrane Zone Genes Coding for Type IV Protocollagen and Laminin by Human Skin Fibroblast in Vitro: Elevated α1(IV) Collagen mRNA Levels in Lipoid Proteinosis. Journal of Investigative Dermatology. 90: 734-738.
Sado, Y., Kagawa, M., Kishiro, Y., Sugihara, K., Naito, I., Seyer, J. M., Sugimoto, M., Oohashi, T. and Ninomiya, Y. 1995. Establishment by the Rat Lymph Node Method of Epitope-defined Monoclonal Antibodies Recognizing the Six Different Alpha Chains of Human Type IV Collagen. Histochemistry and Cell Biology. 104: 267-275.
Pasco S, Brassart B, Ramont L, Maquart FX and Monboisse JC. 2005. Control of Melanoma Cell Invasion By Type IV Collagen. Cancer Detec. Prev. 29: 260-266.
Brash, D. E., Rudolph, J. A., Simon, J. A., Lin, A., Mckenna, G. J., Badent, H. P, & Pontin, J. A. N. 1991. A Role For Sunlight In Skin Cancer : UV-Induced P53 Mutations In Squamous Cell Carcinoma. 88(November): 10124-10128.
Ang, F. A. N. G. W., Ang, Y. U. W., Ing, J. I. E. D., & Ang, J. I. Y. 2005. Detection of Mutations in the COL4A5 Gene by Analyzing Cdna of Skin Fibroblasts. 67: 1268-1274.
Downloads
Published
Issue
Section
License
Copyright of articles that appear in Jurnal Teknologi belongs exclusively to Penerbit Universiti Teknologi Malaysia (Penerbit UTM Press). This copyright covers the rights to reproduce the article, including reprints, electronic reproductions, or any other reproductions of similar nature.
