CYTOTOXICITY EFFECTS OF TYPHONIUM FLAGELLIFORME AND CLINACANTHUS NUTANS ON BREAST CANCER CELLS
DOI:
https://doi.org/10.11113/jt.v77.6902Keywords:
Breast cancer cell (MDA-MB-231), Typhonium flagelliforme and Clinacanthus nutansAbstract
Cancers are diseases that can cause death and breast cancer is one of the prevalence cancers. Conventional treatments have been used to treat cancer. However, these treatments produced inefficient effects and low survival rate. Therefore, most cancer patients began to consume complementary and utilized alternative treatments to alleviate their pain. This study is to identify the cytotoxicity effect of methanolic leaves extract Typhonium flagelliforme and Clinacanthus nutans on breast cancer cells (MDA-MB-231). MTT assay has been used to determine the cytotoxicity effect of both plants on MDA-MB-231 cells and CHO cells (non-cancerous cell), which acted as the positive control cells. Result revealed that T. flagelliforme extract has shown higher cytotoxic effect on MDA-MB-231cells (IC50: 0.11 mg/mL) compared to C. nutans extract (IC50: 0.17 mg/mL). Then, the IC50 values of T. flagelliforme and C. nutans extract on CHO cells were 0.10 mg/mL and 0.24 mg/mL respectively. Based on these results, T. flagelliforme represented higher toxicity effect on CHO compared to the MDA-MB-231 cells. Thus, in future CHO cell (as a positive control) can be replaced with a normal breast cell line such as HCC1395 (epithelial mammary duct of normal breast cells) to understand clearly the toxicity effect of T. flagelliforme extract towards the normal breast cell. Moreover, identification of potential compounds that can inhibit MDA-MB-231 cells growth is also important for future research.
References
Ad’hiah, A. H., Al-Bederi, O. N., and Al-Sammarrae, K. W. 2013. Cytotoxic Effects Of Agrimonia Eupatoria L. Against Cancer Cell Lines In Vitro. Journal of the Association of Arab Universities for Basic and Applied Sciences. 14(1): 87-92.
Agarwal, G., Pradeep, P. V., Aggarwal, V., Yip, C. H., and Cheung, P. S. 2007. Spectrum of Breast Cancer in Asian Women. World J Surg. 31(5): 1031-1040.
Austride, I. 2008. Effect Of 8, 9-Epoxy Eicosapentaenoic Acid On Human Breast Cancer Cell Line MDA-MB-231. Master Dissertation. Retrieved from http://hdl.handle.net/10037/1572.
Ayob, Z., Samad, A. A., and Bohari, S. P. M. 2013. Cytotoxicity Activities in Local Justicia gendarussa Crude Extracts against Human Cancer Cell Lines. Jurnal Teknologi. 64(2).
Cameron, C. S. 1956. The Truth About Cancer: What Is Cancer? Englewood Cliffs: Prentice-Hall.
Chan, L. K., Koh, W. Y. and Tengku-Muhammad, T. S. 2005. Comparison of cytotoxic activities betweenin vitro and field grown plants of Typhonium flagelliforme (Lodd.) blume. Journal of Plant Biology. 48: 25-31.
Chin, J. H., P’ng, X. W. and Akowuah, G. A. 2012 . Acute Oral Toxicity Study Of Clinacanthus Nutans In Mice. International Journal of Pharmaceutical Science and Research. 3(11):4202-4205.
Choo, C. Y., Chan, K. L., Sam, T. W., Hitotsuyanagi, Y. and Takeya, K. 2001a. The Cytotoxicity and Chemical Constituents of the Hexane Fraction of Typhonium flagelliforme (Araceace). Journal of Ethnopharmacology. 77: 129-131.
Choo, C. Y., Chan, K. L., Takeya, K. and Itokawa, H. 2001b. Cytotoxic Activity of Typhonium flagelliforme (Araceae). Phytotherapy Research. 15: 260-262.
Couceiro, M. A., Afreen, F., Zobayed, S. M. A., and Kozai, T. 2006. Variation in Concentrations Of Major Bioactive Compounds Of St. John's Wort: Effects Of Harvesting Time, Temperature And Germplasm. Plant Science. 170(1): 128-134.
Doughari, J. H. 2012. Phytochemicals: Extraction Methods, Basic Structures and Mode of Action as Potential Chemotherapeutic Agents. In Rao, V. (Ed.). Phytochemicals - A Global Perspective of Their Role in Nutrition and Health. Retrieved February 6, 2014 from: http://www.intechopen.com/download/get/type/pdfs/id/32936.
Edmunds, S. C. 2007. The Biology Of Cancer: Genetics and Cancers. In Gabriel, J. 2nd Ed. England: John Wiley and Son Ltd.
Farida, Y., Suwarso, W. P., and Sumaryono, W. 2011. Biological Activity Test of Extracts and Fractions There of Keladi Tikus Leaves Typhonium flagelliforme (Lodd) Blume. The 2nd International Symposium on Temulawak and The 40th National Seminar on Indonesian Medicinal Plants. Bogor-Indonesia on May 26-27.
Farooqui, M, Hassali M. A., Knight A., Shafie A.A, Farooqui, M.A., Saleem, F., Haq, N., and Aljadhey H. 2013. A Qualitative Exploration Of Malaysian Cancer Patients’ Perceptions Of Cancer Screening. BMC Public Health. 13: 48.
Fatemeh, K., and Khosro, P. 2013. In vitro Cytotoxic Activity of Aqueous Root Extract of Althea kurdica against Endothelial Human Bone Marrow Cells (line k562) and Human Lymphocytes. Bull. Env. Pharmacol. Life Sci. 2(6): 23-29.
Gurjar, M. S., Ali, S., Akhtar, M., and Singh, K. S. 2012. Efficacy of Plant Extracts In Plant Disease Management. Agricultural Sciences. 3: 425.
Hosmane, N. S., Maguire, J. A., Zhu, Y. and Takagaki, M. 2012. Boron and Gadolinium Neutron Capture Therapy For Cancer Treatment. World Scientific.
Jayapal, K. P., Wlaschin, K. F., Hu, W., & Yap, M. G. 2007. Recombinant Protein Therapeutics From CHO Cells-20 Years And Counting. Chemical Engineering Progress. 103(10): 40.
Kang, K. S., Morita, I., Cruz, A., Jeon, Y. J., Trosko, J. E., & Chang, C. C. 1997. Expression of Estrogen Receptors In A Normal Human Breast Epithelial Cell Type With Luminal And Stem Cell Characteristics And Its Neoplastically Transformed Cell Lines. Carcinogenesis. 18(2): 251-257.
King, R. J. and Robins, M. W. 2006. Cancer Biology. 3rd edition. Edinburgh Gate, England: Pearson Education.
Lai, C. S., Mas, R. H. M. H., Nair, N. K., Majid, M. I. A., Mansor, S. M. and Navaratnam, V. 2008. Typhonium flagelliforme Inhibits Cancer Cell Growth In Vitro And Induces Apoptosis: An Evaluation By The Bioactivity Guided Approach. Journal of Ethnopharmacology. 118: 14-20.
Lai, C.S., Mas, R. H., Nair, N. K., Mansor, S. and Navaratnam, V. 2010. Chemical Constituents And In Vitro Anticancer Activity of Typhonium flagelliforme (Araceae). Journal of Ethnopharmacology. 127: 486-494.
Lopez, A. D., Mathers, C. D., Ezzati, M., Jamison, D. T., and Murray, C. J. 2006. Global and Regional Burden Of Disease And Risk Factors, 2001: Systematic Analysis Of Population Health Data. The Lancet. 367(9524): 1747-1757.
Lyons, L. 2007. The Biology Of Cancer: Diagnosis and Treatment of Cancer. In Bozzone, D. M. (Ed). New York: Chelsea House.
Machana, S., Weerapreeyakul, N., Barusrux, S., Nonpunya, A., Sripanidkulchai, B., & Thitimetharoch, T. 2011. Cytotoxic and Apoptotic Effects Of Six Herbal Plants Against The Human Hepatocarcinoma (Hepg2) Cell Line. Chin Med. 6: 39.
Manual, S. S. 2007. A Step by Step Guide to Data Analysis using SPSS for Windows. Julie Pallant. 3rd edition. Open University Press.
Mohan, S., Abdul, A. B., Abdelwahab, S. I., AL-Zubairi, A. S., SukariI, M. A., Abdullah, R., Elhassan Taha, M. M., Ibrahim, M. Y. & Syam, S. 2010. Typhonium flagelliforme Induces Apoptosis In Cemss Cells Via Activation Of Caspase-9, PARP Cleavage And Cytochrome C Release: Its Activation Coupled With G0/G1 Phase Cell Cycle Arrest. Journal of Ethnopharmacology. 131: 592-600.
Moongkarndi, P., Kosem, N., Kaslungka, S., Luanratana, O., Pongpan, N., and Neungton, N. 2004. Antiproliferation, Antioxidation And Induction Of Apoptosis By Garcinia Mangostana (Mangosteen) On SKBR3 Human Breast Cancer Cell Line. Journal of Ethnopharmacology. 90(1): 161-166.
Mosmann, T. 1983. Rapid Colorimetric Assay For Cellular Growth And Survival: Application To Proliferation And Cytotoxicity Assays. Journal of Immunological Methods. 65: 55-63.
Pieroni, A. and Vandebroek, I. 2007. Traveling Cultures and Plants: The Ethnobiology And Ethnopharmacy Of Human Migrations. New York: Berghahn Books.
Rates, S. 2001. Plants As Source Of Drugs. Toxico. 39: 603-613.
Ridley, H. N. 1967. The Flora of the Malay Peninsula: Monocotyledons (concluded), Gymnospermeae and General Indices, London: V. L. Reeve and Co. Ltd.
Sadeghi-Aliabadi, H., Sajjadi, S. E., and Khodamoradi, M. 2009. Cytotoxicity of Euphorbia Macroclada On MDA-MB-468 Breast Cancer Cell Line. Iranian Journal of Pharmaceutical Sciences. 5(2): 103-108.
Society A. C. 2009. Breast Cancer Facts & Figures 2009-2010. Atlanta: American Cancer Society.
Teo, C. K. H., and Ch’ng, B. I. 1999. Cancer - Yet They Live, revised ed. Care C.A., Penang, Malaysia.
Wirotesangthong, M. and Rattanakiat, S. 2006. Anti-herpes Simplex Virus Type 2 Activities Of Some Thai Medicinal Plants. Thai Journal Pharmacy Science. 30: 19-27.
Wolfgang, A. S. 2005. Molecular Biology Of Human Cancer An Advanced Student’s Textbook. Breast Cancer. Springer. North, Central and South America.
Workman, L. 2006. Cancer Therapies: The Biological Basis of Cancer. In Barton-Burke, M. (Ed.). London: Jones and Bartlett.
Yip, C. H., Taib, N. A. M., and Mohamed, I. 2006. Epidemiology of Breast Cancer in Malaysia. Asian Pacific Journal of Cancer Prevention. 7.
Yong, Y. K., Tan, J. J., Teh, S. S., Mah, S. H., Ee, G. C. L., Siong, H., Chiong, Z. A. and Ahmad, Z. 2013. Clinacanthus nutans Extracts are Antioxidant with Anti-proliferative Effect on Cultured Human Cancer Cell Lines. Evidence-Based Complementary and Alternative Medicine. 8.
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