• Che Puteh Osman Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia
  • Nor Hadiani Ismail Atta-ur-Rahman Institute for Natural Product Discovery, Universiti Teknologi MARA, 42300 Bandar Puncak Alam, Selangor, Malaysia
  • Rohaya Ahmad Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia
  • Noorjahan Banu Alitheen Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
  • Mohamad Jemain Mohamad Ridwan Kolej Sains Kesihatan Bersekutu Sultan Azlan Shah, 31250 Tanjung Rambutan, Ulu Kinta, Perak, Malaysia
  • Talat Maakhmor Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan



Rennellia elliptica, anthraquinones, antioxidant, antidiabetic, cytotoxic


Rennellia elliptica is a small tropical shrub that commonly known as ‘segemuk’. The root decoction is traditionally used for health vitality. We have previously isolated Rubia-type anthraquinones from the root extract of Rennellia elliptica. The root extract is a potent antiplasmodial and some of the anthraquinones were strong inhibitors with the IC50 values of less than 2 µM. In continuation of our investigation on the pharmacological potential of R. elliptica, the root extracts and the anthraquinones were screened for antioxidant, antidiabetic and cytotoxic activities. Dichloromethane extract is a strong lipid peroxidation inhibitor but showed no activity against 3T3, MCF7 and 4T1 cancer cell lines and α-glucosidase. The anthraquinones showed weak radical scavenging activities against DPPH radicals. 2-Formyl-3-hydroxy-9,10-anthraquinones, damnacanthal and 2-methyl-3-hydroxy-9,10-anthraquinones showed moderate activity when screened for cytotoxicity using 3T3 cell lines at 30 µg/ml with 74.15, 67.34 and 50.40 %, respectively. When screened using 4T1 and MCF7 cell lines, only 2-formyl-3-hydroxy-9,10-anthraquinone showed moderate cytotoxicity after 72 hours of incubation. 1,2-Dimethoxy-6-methyl-9,10-anthraquinones and damnacanthal showed weak α-glucosidase inhibitory activity with 21.3 and 19.9 %, respectively, when screened at 10 µg/ml. 


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