IDENTIFICATION AND CLASSIFICATION OF BACTERIAL AUTOTRANSPORTERS
DOI:
https://doi.org/10.11113/jt.v78.8242Keywords:
Pathogenicity, bacterial autotransporter, adhesions, esterase, proteasesAbstract
Bacterial Autotransporters is a huge and assorted super family of proteins present in external boundary of the gram negative bacteria. Autotransporters consists of C-terminal beta domain, N-terminal passenger domain and a pathogenic function. The beta domain of the bacterial autotransporter is associated with pathogenicity with three types of functions namely adhesions, esterase and proteases. Identification and classification of a bacterial autotransporter is important in order to target it and hinder its ability to cause disease. Identification and classification therefore has become a challenging problem due to its homology sequence. In this paper, we ought to establish a web based tool that could identify and classify bacterial autotransporters on basis of homology with already reported bacterial autotransporters. The input required for tool amino acid sequence in FASTA format. The tool will analyze the query based on similarity with already reported sequence in database and generate output with information in terms of function, organism, class, length, protein ID and symbol. Further, this user friendly and freely accessible tool is integrated with NCBI, InterPro, Pfam, pubmed. Moreover, newly detected bacterial autotransporter can be easily added in the Bacterial Autotransporter Detector tool database with authentic referenceReferences
Celik, N., C. T. Webb, D. L. Leyton, K. E. Holt, E. Heinz, R. l. Gorrel, T. Kwok, T. Naderer, R. A. Strugnell, T. P. Speed, R. D. Teasdale, V. A. Likić, and T. Lithgow. 2012. A Bioinformatic Strategy For The Detection, Classification And Analysis Of Bacterial Autotransporters. PLoS One. 7(8): e43245,
Leo, J. C., I. Grin, and D. Linke. 2012. Type V Secretion: Mechanism(S) Of Autotransport Through The Bacterial Outer Membrane. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 367(1592): 1088–101.
Henderson, I. R., F. Navarro-Garcia, and J. P. Nataro. 1998. The Great Escape: Structure And Function Of The Autotransporter Proteins. Trends Microbiol. 6: 370-378.
Oliver, D. C., G. Huang, E. Nodel, S. Pleasance, and R. C. Fernandez. 2003. A Conserved Region Within The Bordetella Pertussis Autotransporter Brka Is Necessary For Folding Of Its Passengerdomain. Mol. Microbiol. 47: 1367-1383.
Corpet, F., F. Servant, J. Gouzy, and D. Kahn. 2000. ProDom and ProDom-CG: Tools For Protein Domain Analysis And Whole Genome Comparisons. Nucleic Acids Res. 28: 267-269
Bernstein, H. D. 2007. Are Bacterial “Autotransporters‟ Really Transporters?. Trends Microbiol. 15(10): 441–447.
Benz and M. A. Schmidt. 2011. Structures And Functions Of Autotransporter Proteins In Microbial Pathogens. Int. J. Med. Microbiol. 301(6): 461–468.
Leo, J. C., I. Grin, and D. Linke. 2012. Type V Secretion: Mechanism(s) of Autotransport Through The Bacterial Outer Membrane. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 367(1592): 1088–1101.
Parton, R. Review Of The Biology of Bordetella pertussis. Biologicals. 1999. 27: 71 –76.
Charles, I. G., G. Dougan, D. Pickard, S. Chatfield, M. Smith, P. Novotny, P. Morrissey, N. F. Fairweather. 1989. Molecular Cloning And Characterization Of Protective Outer Membrane Protein P.69 From Bordetella Pertussis. Proc Natl Acad Sci USA. 86(10): 3554–3558.
Li, L. J., G. Dougan, P. Novotny, I. G. Charles. 1991. P.70 Pertactin, An Outer-Membrane Protein From Bordetella Parapertussis: Cloning, Nucleotide Sequence And Surface Expression in Escherichia coli. Mol Microbiol. 5(2): 409–417.
Li, J., N. F. Fairweather, P. Novotny, G. Dougan, I. G. Charles. 1992. Cloning, Nucleotide Sequence And Heterologous Expression Of The Protective Outer-Membrane Protein P.68 Pertactin from Bordetella bronchiseptica. J Gen Microbiol. 138(8): 1697–1705.
Charles, I., N. Fairweather, D. Pickard, J. Beesley, R. Anderson, G. Dougan, M. Roberts. 1994. Expression of the Bordetella pertussis P.69 Pertactin Adhesin In Escherichia Coli: Fate Of The Carboxy-Terminal Domain. Microbiology. 140(12): 3301–3308.
Figura, N. 1997. Helicobacter Pylori Factors Involved In The Development Of Gastroduodenal Mucosal Damage And Ulceration. J Clin Gastroenterol. 25: S149–S163.
Gerhard, M, N. Lehn, N. Neumayer, T. Boren, R. Rad, W. Schepp, S. Miehlke, M. Classen, C. Prinz. 1999. Clinical Relevance Of The Helicobacter Pylori Gene For Blood-Group Antigen-Binding Adhesin. Proc Natl Acad Sci USA. 96(2): 12778-12783
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