ANTIDIABETIC EFFECTS OF KNEMA GLAUCA LEAF EXTRACT TOWARD INHIBITIONS OF Α-AMYLASE AND Α-GLUCOSIDASE ASSAYS
DOI:
https://doi.org/10.11113/jt.v78.8520Keywords:
Postprandial hyperglycaemia, Knema glauca leaf, α-amylase, α-glucosidaseAbstract
This study was part of our preliminary work in the search for biologically active compounds from endemic plants of Kuala Keniam National Park, Pahang, Malaysia. Postprandial hyperglycaemia is linked to the development of diabetes complications such as micro and macro vascular diseases. Inhibitions of carbohydrate digestive key enzymes, α-amylase and α-glucosidase using plant extracts are possible therapeutic strategies to suppress postprandial hyperglycaemia. The aim of this study was to investigate the effects of the dichloromethane leaf extract of Knema glauca (DLKG) in vitro and in vivo α-amylase and α-glucosidase inhibitory activities. The antidiabetic property of DLKG was investigated by using in vitro α-amylase and α-glucosidase inhibitory activity assays. The effects of DLKG on oral starch and sucrose challenge tests were investigated by administering 125, 250 and 500 mg/kg of the extract to normal and diabetic rats. DLKG demonstrated potent α-amylase and α-glucosidase inhibitory activities with IC50 values of 1.26 and 4.09 µg/ml, respectively. In the oral starch challenge test, the extract significantly (p<0.05) reduced blood glucose levels in both normal and diabetic rats. On the other hand, DLKG showed significant (p<0.05) reduction of blood glucose levels in normal rats in the oral sucrose challenge test. The results showed that the leaves of K. glauca possessed a beneficial effect in reducing postprandial hyperglycaemia and have potential as an alternative antidiabetic agent.
References
Krentz, A. J., Clough, G., and Byrne, C. D. 2007. Interaction Between Microvascular And Macrovascular Disease In Diabetes: Pathophysiology And Therapeutic Implications. Diabetes Obesity Metabolism. 9(6): 781-791.
Rahimzadeh, M., Jahanshahi, S., Moein, S., Moein and M.R. 2014. Evaluation Of Α-Amylase Inhibition By Urtica Dioica And Juglan Regia Extracts. Iran J Basic Med Sci. 17: 465-469.
Dods, R. F. 2013. Understanding Diabetes: A Biochemical Perspective. New York: John Wiley & Sons.
Tundis, R., Loizzo, M. R., Menichini, F. 2010. Natural Products As A-Amylase And A-Glucosidase Inhibitors And Their Hypoglycaemic Potentials In The Treatment Of Diabetes: An Update. Mini Reviews in Medicinal Chemistry. 10(4): 315-331.
Sneha, J. A., and Sanjay, C. 2011. Alpha Amylase Inhibitory And Hypoglycemic Activity Of Clerodendrone Multiflorum Linn. Stems. Asian Journal of Pharmaceutical and Clinical Research. 4(2): 99-102.
Bedekar, A., Shah, K. and Koffas, M. 2010. Natural Products For Type II Diabetes Treatment. Advances in Applied Microbiology. 71: 21-73.
Olubomehin, O. O., Abo, K. A., and Ajaiyeoba, E. O. 2013. Alpha-amylase Inhibitory Activity Of Two Anthocleista Species And In Vivo Rat Model Anti-Diabetic Activities Of Anthocleista Djalonensis Extracts. Journal of Ethnopharmacology. 146(3): 811-814.
Ryu, H. W., Cho, J. K., Curtis-Long, M. J., Yuk, H. J., Kim, Y. S., Jung, S., Kim, Y. S., Lee, B. W., and Park, K. H. 2011. α-Glucosidase Inhibition And Antihyperglycemic Activity Of Prenylated Xanthones From Xanthones From Garcinia mangostana. Phytochemistry. 72(17): 2148-2154.
Kazeem, M. I., Adamson, J. O., and Ogunwande, I.A. 2013. Mode Of Inhibition Of Α-Amylase And Α-Glucosidase By Aqueous Extract of Morinda lucida Benth leaf. Biomedical Research International. 2013: 527570.
Rangkaew, N., Suttisri, R., Moriyasu, M., and Kawanishi, K. 2009. A New Acyclic Diterpene And Bioactive Compounds From Knema glauca. Archives of Pharmacal Research. 32(5): 685-692.
Ali, H., Houghton, P. J., and Soumyanath, A. 2006. α-Amylase Inhibitory Activity Of Some Malaysian Plants Used To Treat Diabetes; With Particular Reference To Phyllanthus amarus. Journal of Ethnopharmacology. 107(3): 449-55.
Lee, S. S., Lin, H. C., and Chen, C. K. 2008. Acylated Flavanol Monorhamnosides, Α-Glucosidase Inhibitors, From Machilus philippinensis. Phytochemistry. 69(12): 2347-2353.
Subramanian, R., Zaini, M. A., and Amirin, S. 2008. In Vitro Α-Glucosidase And Α-Amylase Enzyme Inhibitory Effects Of Andrographis Paniculata Extract And Andrographolide. Acta Biochimica Polonica. 55(2): 391-398.
Das, S., Das, S. and De, B. 2012. In Vitro Inhibitions Of Key Enzymes Related To Diabetes By The Aqueous Extracts Of Some Fruits Of West Bengal, India. Current Nutrition and Food Science. 8(1): 19-24.
Copeland, R. A. 2013. Evaluation Of Enzyme Inhibitors In Drug Discovery: A Guide For Medicinal Chemists And Pharmacologists. 2th Edition. New York: John Wiley & Sons. 15-20.
Lee, A., Kang, M., Choe, E., and Kim, J. 2015. Hypoglycemic And Antioxidant Effects Of Doraesoon (Actinidia Arguta Shoot) In Animal Models Of Diabetes Mellitus. Nutrition Research and Practice. 9(3): 262-267.
Rios, J. L., Francini, F., and Schinella, G. R. 2015. Natural Products For The Treatment Of Type 2 Diabetes Mellitus. Planta Medica. 81: 975-994.
Chiasson, J. L. 2006. Acarbose For The Prevention Of Diabetes, Hypertension And Cardiovascular Disease In Subjects With Impaired Glucose Tolerance: Study to Prevent Non-Insulin-Dependent Diabetes Mellitus (STOP-NIDDM) Trial. Endocrine Practice. 12(1): S25-S30.
Liu, L., Yu, Y. L., Liu, C., Wang, X. T., Liu, X.D., and Xie, L., 2011. Insulin Deficiency Induces Abnormal Increase In Intestinal Disaccharidase Activities And Expression Under Diabetic States, Evidences From In Vivo And In Vitro Study. Biochemical Pharmacology. 82(12): 1963-1970.
El-Amin, Virk, P., Elobeid M. A., Almarhoon, Z. M., Hassan, Z. K., Omer, S. A, Merghani, N. M., Daghestani, M. H., Al-Olayan, E. M. 2013. Antidiabetic effect of Murraya koenigii (L) and Olea europaea (L) leaf Extracts On Streptozotocin-Induced Diabetic Rats. Pak J Pharm Sci. 26: 359-365.
Li Liu, Yun-Li Yu, Can Liu, Xin-Ting Wang, Xiao-Dong Liu, Lin Xie. 2011. Insulin Deficiency Induces Abnormal Increase In Intestinal Disaccharidase Activities And Expression Under Diabetic States, Evidences From In Vivo And In Vitro Study. Biomedical Pharmacology. 82(12): 1963-1970.
Manzari-Tavakoli, A., Pouraboli, I., Yaghoobi, M.M., Mehrabani, M., and Mirtadzadini, S.M. 2013. Antihyperglycemic, Antilipid Peroxidation, And Insulin Secretory Activities Of Otostegia Persica Shoot Extract In Streptozotocin-Induced Diabetic Rats And In Vitro C187 Pancreatic β-cells. Pharmaceutical Biology. 51: 253-259.
Badole, S. L., and Bodhankar, S. L. 2007. Interaction Of Aqueous Extract Of Pleurotus ulmonarius (Fr.) Quel.-Champ. With Acarbose In Alloxan Induced Diabetic Mice. Journal of Application Biomedicine. 3(5): 157-166.
Picot, C. M., Subratty, A. H., Mahomoodally, M. F. 2014. Inhibitory Potential Of Five Traditionally Used Native Antidiabetic Medicinal Plants On Α-Amylase, Α-Glucosidase, Glucose Entrapment, And Amylolysis Kinetics In Vitro. Advances In Pharmacological Sciences. 739834.
Lawag, I. L., Aguinaldo, A. M., Naheed, S. and Mosihuzzaman, M. 2012. Alpha-glucosidase Inhibitory Activity Of Selected Philippine Plants. Journal of Ethnopharmacology. 144: 217-219.
Sawadjoon, S., Kittakoop, P., Kirtikara, K., Vichai, V., Tanticharoen, M., and Thebtaranonth, Y. 2002. Atropisomeric Myristinins: Selective COX-2 Inhibitors And Antifungal Agents From Myristica Cinnamomea. Journal of Organic Chemistry. 67(16): 5470-5475.
Maloney, D. J., Deng, J. Z., Starck, S. R., Gao, Z., and Hecht, S. M. 2005. (+)-Myristinin A, A Naturally Occurring DNA Polymerase Beta Inhibitor And Potent DNA- Damaging Agent. Journal of the American Chemical Society. 27(12): 4140-4141.
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