MORPHOMETRIC BRAIN MEASUREMENT OF PRENATAL ULTRASOUND-INDUCED RABBIT FETUS USING MICRO-COMPUTED TOMOGRAPHY (MICRO-CT)
DOI:
https://doi.org/10.11113/jt.v78.9077Keywords:
Brain, fetal, gestation, in-utero, micro-CT, morphometric, prenatal, ultrasoundAbstract
Prenatal ultrasound is widely used and became an integral part of the clinical practice, particularly in obstetrics and gynecology. However, the advances in the capability of ultrasound equipment nowadays, trigger a greater image processing power, hence might as well increase the fetal exposure. Hence premises the morphometric brain measurement to be assessed in this study as an evidence of the ultrasound interruptions during the fetal neuronal development. This research randomly analyzed a total of 18 ultrasound-induced rabbit fetuses for fetal brain volume and surface after being exposed prenatally to the ultrasound exposure (duration=60 minutes; frequency = 7.09 MHz; spatial peak temporal average intensity (ISPTA) = 49.4 W/cm2; power = 56.0 W; thermal index (TI) = 0.2; mechanical index (MI) = 1.0). The fetuses were analyzed for morphometric brain measurement of brain volume and surface using Skyscanâ„¢ 1176 Micro-computed Tomography (Micro-CT). There were significant differences in the measurement of brain volume and surface at the 2nd and 3rd stage of gestation (P < 0.05). Results suggested that there are significant differences in the brain volume and surface between the controls and the 2nd and 3rd stage of gestation. There are also a significant reduction in the brain volume and surface in the exposed groups at all stages of gestation (P < 0.05).Â
References
Saari-Kemppainen A., Karjalainen O., Ylöstalo P. and Heinonen O. P. 1990. Ultrasound Screening and Perinatal Mortality: Controlled Trial of Systematic One-Stage Screening in Pregnancy. The Helsinki Ultrasound Trial. Lancet. 336(8712): 387-391.
Woodcock, J. P. 1979. Ultrasonics: Medical Physics Handbooks. Bristol: Adam Higler.
Abramowicz, J. S., Lewin, P. A., and Goldberg, B. B. 2008. Ultrasound Bioeffects for the Perinatologist. Glob. Libr. Women’s Med.
Burd, I., Srinivas, S., Pare, E., Dharan, V. and Wang, E. 2009. Is Sonographic Assessment of Fetal Weight Influenced by Formula Selection? J. Ultrasound Med. 28: 1019-1024.
Johnsen, S. L., Rasmussen, S., Sollien, R., and Kiserud, T. 2004. Fetal Age Assessment Based on Ultrasound Head Biometry And Effect of Maternal and Fetal Factors. Acta Obstet. Gynegologica Scand. 83: 716-723.
Zhang, J., Sundaram, R., Sun, W., and Troendle, J. 2008. Fetal Growth and Timing of Parturition in Humans. Am. J. Epidemiol. 168: 946-951.
Sonek, J. 2007. First Trimester Ultrasonography in Screening and Detection of Fetal Anomalies. Am. J. Med. Genet. Part C (Seminars Med. Genet. 145: 45-61.
McHugo, J. 2000. Obstetric and Gynecology Ultrasonography. Aids To Radiological Differential Diagnosis. 3rd ed., S. Chapman and R. Nakielny, Eds. UK: W. B. Saunders, 2000. 480-504.
John, H. M. 1996. The Central Nervous System. in Neuroanatomy: Text and Atlas. 3rd ed. McGraw-Hill. 1-25.
Pomeroy, S. L. and Ulrich, N. J. 2004. Neurology: Development of Nervous System. in Fetal and Neonatal Physiology. 3rd ed. R. A. Polin, W. W. Fox, and S. H. Abman, Eds. Philadelphia, Pennsylvania: Saunders. 2: 1675-1698.
Kieler, H., Cnattingius, S., Haglund, B., Palmgren, J., and Axelsson, O. 2001. Sinistrality- A Side-Effect Of Prenatal Sonography: A Comparative Study Of Young Men. Epidemiology. 12(6): 618-623.
Ovalle, W. K. and Nahirney, P. C. 2008. Nervous Tissue. Netter’s Essential Histology. Saunders Elsevier. 101-130.
Swerdlow, A. J. 2010. Health Effects Of Exposure To Ultrasound And Infrasound: Report Of The Independent Advisory Group On Non-Ionising Radiation. UK. 1-196.
Zaiki, F. W. A. and Dom, S. M. 2014. The Effect Of Prenatal Ultrasound Heating Throughout Gestation On Rabbit Fetal Weight. Int. J. Bio-Science Bio-Technology. 6(3): 71-80.
Lebas, F., Coudert P., Rouvier R., and de Rochambeau H. 1986. The Rabbit: Husbandry, Health And Production. Food and Agriculture Organization (FAO) Animal Production and Health Series. 21.
Metscher, B. D. 2009. MicroCT For Comparative Morphology: Simple Staining Methods Allow High-Contrast 3D Imaging Of Diverse Non-Mineralized Animal Tissues. BMC Physiol. 9(11): 1-14.
Edwards, M. J., Saunders, R. D., and Shiota, K. 2003. Effect Of Heat On Embryos And Foetuses. Int. J. Hyperth. 19(3): 295-324.
Bicher, H. I., Mitagvaria, N., Devdariani, M., Davlianidze, L., Nebieridze, M., and Momtselidze, N. 2013. Autoregulation Of The Brain Temperature During Whole Body Hyperthermia. Conf. Pap. Med. 1-5.
Sharma, H. S. 2006. Hyperthermia Induced Brain Oedema: Current Status & Future Perspectives. Indian J. Med. Res. 629-652.
Suneetha, N. and Kumar, R. P. S. 1993. Ultrasound-induced Enhancement Of ACh, AChE and GABA in Fetal Brain Tissue Of Mouse. Ultrasound Med. Biol. 19(5): 411-413.
Zaiki, F. W. A., Dom, S. M., Razak, H. R. A., and Hassan, H. F. 2013. Prenatal Ultrasound Heating Impacts On Fluctuations In Haematological Analysis Of Oryctolagus Cuniculus. Quant. Imaging Med. Surg. 3(5): 262-8.
Gent, R. 1997. Biological Effects And Safety Of Diagnostic Ultrasound. Applied Physics And Technology Of Diagnostic Ultrasound. 1st ed. Australia: Milner. 301-316.
Duck, F. A. 2008. Hazards , Risks And Safety Of Diagnostic Ultrasound. Med. Eng. Phys. 30: 1338-1348.
Duck, F. A. 2003. Safety Aspects Of The Use Of Ultrasound In Pregnancy. Cambridge Journals Online. 14: 1-21.
Harcourt-Brown, F. 2002. Textbook Of Rabbit Medicine. UK: Reed Educational and Professional Publishing.
Zaiki, F. W. A., Dom, S. M., Razak, H. R. A., and Hassan, H. F. 2013. Prenatal Ultrasound Heating Influences On Fetal Weight Assessment Of Oryctolagus Cuniculus Throughout Pregnancy. Proceedings International Workshop of Bioscience and Medical Research 2013. December 11-13, 2013. Jeju Island, Korea. 33: 5-11.
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