THE EFFECTS OF PHYLLANTHUS GOMPHOCARPUS HOOK. F. AQUEOUS EXTRACT SUPPLEMENTATION ON ANDROGENIC HORMONAL LEVEL AND HISTOLOGICAL TESTES MORPHOMETRY
DOI:
https://doi.org/10.11113/jt.v78.9084Keywords:
Phyllanthus gomphocarpus Hook. F, Bisphenol A (BPA), androgenic hormones, testes morphometric, spermatogenesisAbstract
Phyllanthus gomphocarpus Hook. F is a herb found in tropical rainforest countries and has been widely used in traditional medicine especially in Peninsular Malaysia to treat male infertility problems and increase human health. Ten percent aqueous extraction of PGR was prepared by heating in 40º C for 12 hours and its decoction were freeze dried to obtain crude extract. Total numbers of 24 male rats were randomly divided into 4 groups which NEC, POC, PGR50 and PGR800. Except for NEC group, other groups were induced genitotoxicity by oral supplementation of 200 mg/kg of BPA for 21 consecutive days. For PGR50 and PGR800 groups, the animals were concomitantly supplemented with 50 mg/kg and 800 mg/kg of PGR extracts respectively. Blood and testes sample were collected for hormonal and testes morphometric analysis. Results clearly showed that that testosterone (T), the most important hormone in spermatogenesis process were significantly increased in PGR supplemented groups (p<0.05) compared to NEC and POC groups. For the level of DHT, FSH, and LH hormonal level, the results were varies and can be explained with the deterioration effects of BPA and the hormonal regulations itself. Testes morphometric study showed no significant difference in the seminiferous tubule diameter in each groups but was significantly increased (p<0.05) in the epithelium height, which indicates higher rate of spermatogenesis process. In addition, PGR possessed a protective effect against the toxicity effects of BPA. In conclusion, PGR was suggested to enhance male fertility through improvement of androgenic hormones and possessed a protective effect against genitotoxicity of BPA.
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