OPTIMIZATION OF COCOA BUTTER EQUIVALENT PRODUCTION FROM FORMULATED HARD PALM OIL MID-FRACTION AND CANOLA OIL BLENDS
DOI:
https://doi.org/10.11113/jt.v78.9243Keywords:
Cocoa butter equivalent, hard palm oil mid-fraction, canola oil, enzymatic interesterificationAbstract
The study to find cocoa butter equivalent (CBE) as an alternative to cocoa butter (CB) from available and low cost commercial oils or fats has been increased recently. Current study investigates the blending of hard palm oil mid-fraction (PMF) with canola oil to produce high nutritional CBE using immobilized lipase from Rhizomucor miehei. The experiments were designed using Response Surface Methodology (RSM) to optimize the percentage of saturated-unsaturated-saturated (StUSt) triacylglycerols (TAGs). The experiment was performed at hard PMF concentration of 50 to 90% (w/w), lipozyme load between 5% and 10% (based on the weight of substrate) with a reaction time between 2 to 14 hours. The best reaction conditions to attain this target was 89.35% (w/w) of hard PMF concentration, 2 hours of reaction time, and 5% (based on the weight of substrate) of lipozyme load, resulting CBE which contains 64.44±1.18% of StUSt. The addition of canola oil improved the nutritional value of CBE which was marked by the higher percentage of linoleic acid (omega-6, 4.53±0.06%) and linolenic acid (omega-3, 0.74±0.14%) in CBE than CB (omega-6, 2.68±0.34%). Enzymatic interesterification was not altering fatty acid content in the CBE, especially linoleic acid (omega-6) and linolenic acid (omega-3) which was characterized by no significant difference (p > 0.05) between the fatty acid profile of initial mixture (before interesterification) and CBE (after interesterification).
References
Lipp, M., Simoneau, C., Ulberth, F., Anklam, E., Crews, C., Brereton, P., De Greyt, W., Schwack, W., and Wiedmaier, C. 2001. Composition of Genuine Cocoa Butter and Cocoa Butter Equivalents. Journal of Food Composition and Analysis. 14(2001): 399-408.
Wang, H. X., Wu, H., Ho, C. T. and Weng, X. C. 2006. Cocoa Butter Equivalent from Enzymatic Interesterification of Tea Seed Oil and Fatty Acid Methyl Esters. Food Chemistry. 97(2006): 661-665.
Liu, K. J., Cheng, H. M., Chang, R. C., and Shaw, J. F. 1997. Synthesis of Cocoa Butter Equivalent by Lipase-Catalyzed Interesterification In Supercritical Carbon Dioxide. Journal of American and Oil Chemist’ Society. 74(11): 1477-1482.
Simmons, R. 2011. World Cocoa and CBE Markets: LMC International. Retrieved October 01, 2013. from www.lmc.co.uk, 21th June 2011.
International Cocoa and Commodities Organisation. 2013. World Cocoa Bean Production, Grindings and Stocks. Quarterly Bulletin of Cocoa Statistic vol. XXXIX, no 2 Cocoa Year 2012/2013. 39:13.
Jahurul, M. H. A., Zaidul, I. S. M., Norulaini, N. A. N., Sahena, F., Jinap, S., Azmir, J., Sharif, K. M., Mohd Omar, A. K. 2013. Cocoa Butter Fats and Possibilities of Substitution in Food Products Concerning Cocoa Varieties, Alternative Sources, Extraction Methods, Composition, and Characteristics. Journal of Food Engineering. 117(2013): 467-476.
David Illingworth. 2002. Fractionation of Fats. In Alejandro G. Marangoni and Suresh S. Narine(eds.)Physical Properties of Lipids. New York: Marcel Dekker Inc.
Khumalo, L. W., Majoko, L., Read, J. S., and Ncube, I. 2002. Characterisation of Some Underutilised Vegetable Oils and Their Evaluation as Starting Materials for Lipase-Catalysed Production of Cocoa Butter Equivalents. Journal of Industrial Crops and Products. 16(2002): 237-244.
R. Prsybylski, T. Mag, N. A. M. Eskin, and B. E. McDonald. 2005. Canola oil. In Fereidoon Shahidi (ed.) Bailey’s Industrial Oil and Fat Products, 6 Volume Set. 6th Edition. London: John Wiley and Sons Inc.
Derrick Rousseau and Alejandro G. Marangoni. 2002. Chemical Interesterification of Food Lipids: Theory and Practice. In Casmir C. Akoh and David B. Min (eds.) Food Lipids: Chemistry, Nutrition, and Biotechnology. Second Edition, Revised and Expanded. New York: Marcel Dekker Inc.
Wendy M. Willis and Alejandro G. Marangoni. 2002. Enzymatic Interesterification. In Casmir C. Akoh and David B. Min (eds.). Food Lipids: Chemistry, Nutrition, and Biotechnology. Second Edition, Revised and Expanded. New York: Marcel Dekker Inc.
Chang, M. K., Abraham, G. and John, V. T. 1990. Production of Cocoa Butter-Like Fat from Interesterification of Vegetable Oils. Journal of American and Oil Chemist’ Society. 67(11): 832-834.
Soekopitojo, S. 2011. Interesterifikasi Enzimatik Bahan Baku Berbasis Minyak Sawit untuk Produksi Cocoa Butter Equivalents. Institut Pertanian Bogor, Ph.D. Thesis.
Naessens, L. 2012. Production of Cocoa Butter Equivalent Through Enzymatic Acidolysis. Gent University, Master. Thesis.
Kadivar, S., De Clerq, N., de Walle, D. V., and Dewettinck, K. 2014. Optimisation of Enzymatic Synthesis of Cocoa Butter Equivalent from High oleic Sunflower Oil. Journal of the Science of Food and Agriculture. 94(7): 1325-1331.
Nielsen, S. S. 2010. Food Analysis Laboratory Manual. Second Edition. New York: Springer.
Whitaker, J. 2001. Current Protocols in Food Analytical Chemistry. California: John Wiley and Sons Inc.
Soekopitojo, S., Hariyadi, P., Muchtadi, T. R., and Andarwulan, N. 2009. Enzymatic Interesterification of Palm Oil Midfraction Blends for the Production of Cocoa Butter Equivalents. Asian Journal of Food Agro-Industry. 2(04): 807-816.
Li, D., Adhikari, P., Shin, J. A., Lee, J. H., Kim, Y. J., Zhu, X. M., Hu, J. N., Jin, J., Akoh, C. C., Lee, K. T. 2010. Lipase-Catalyzed Interesterification of High Oleic Sunflower Oil and Fully Hydrogenated Soybean Oil Comparison of Batch and Continuous Reactor for Production of Zero Trans Shortening Fats. LWT – Food Science and Technology. 43(2010): 458-464.
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