A KINETIC STUDY OF ENZYMATIC HYDROLYSIS OF OIL PALM BIOMASS FOR FERMENTABLE SUGAR USING POLYETHYLENE GLYCOL (PEG) IMMOBILIZED CELLULASE

Authors

  • Umi Aisah Asli Centre of Hydrogen Energy, Institute of Future Energy, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Malaysia
  • Isah Nwaha Faculty of Chemical & Energy Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Malaysia
  • Hazirah Hamid Faculty of Chemical & Energy Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Malaysia
  • Zainul Akmar Zakaria Institute of Bioproduct Development, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Malaysia
  • Aziatul Niza Sadikin Faculty of Chemical & Energy Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Malaysia
  • Mohd Johari Kamaruddin Centre of Hydrogen Energy, Institute of Future Energy, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Malaysia

DOI:

https://doi.org/10.11113/jt.v78.9565

Keywords:

Oil Palm Empty Fruit Bunch (EFB), hydrolysis, fermentable sugars, immobilized cellulase, Glutaraldehyde Coupling Method

Abstract

In this work, enzymatic hydrolysis by cellulase in a soluble and an immobilized form was studied to convert lignocellulosic oil palm empty fruit bunch (EFB) biomass into fermentable sugars as a feedstock for bioethanol production. The cellulase was covalently immobilized with activated and functionalized polyethylene glycol (PEG) via glutaraldehyde coupling method. As a whole, the immobilized cellulase displayed 50% higher efficiency over free cellulase, in reducing sugar recovery during hydrolysis reactions at pH of 4.8 and temperature of 50°C. From the kinetic study, it showed that Michaelis constant (Km) and limiting velocity (Vmax) of immobilized cellulase were 179.2 mg/ml and 33.5 mg/ml.min respectively, comparable with the value for free cellulose, 171.8 mg/ml and 34.5 mg/ml.min respectively. This result could be attributed to the effect of PEG on the binding cellulase to substrate desorb substrates, and enables free interaction of cellulase to hydrolyse cellulose maximally

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Published

2016-08-10

How to Cite

A KINETIC STUDY OF ENZYMATIC HYDROLYSIS OF OIL PALM BIOMASS FOR FERMENTABLE SUGAR USING POLYETHYLENE GLYCOL (PEG) IMMOBILIZED CELLULASE. (2016). Jurnal Teknologi, 78(8-3). https://doi.org/10.11113/jt.v78.9565