FABRICATION AND TESTING OF POLYDIMETHYLSILOXANE (PDMS) MICROCHANNEL FOR LAB-ON-CHIP (LOC) MAGNETICALLY-LABELLED BIOLOGICAL CELLS SEPARATION
DOI:
https://doi.org/10.11113/jt.v78.9587Keywords:
Microfluidics channel, Lab-on-Chip (LoC), polydimethylsiloxane (PDMS), biological cells, microbeadsAbstract
Microfluidics channel of micron- to millimeter in dimension has been widely used for fluid handling in transporting, mixing and separating biological cells in Lab-on-Chip (LoC) applications. In this research, fabrication and testing of Polydimethylsiloxane (PDMS) microfluidic channel for Lab-on-chip magnetically-labelled biological cells separation is presented. The microchannel is designed with one inlet and outlet. A reservoir or chamber is proposed as an extra component of the microchannel design for ease of trapping the intended biological cells in LoC magnetic separator system. The PDMS microchannel of circular-shaped chamber geometry has been successfully fabricated using replica molding technique from SU-8 negative photoresist mold. An agglomerate-free microbeads flowing has been observed using the fabricated PDMS microchannel. Trapping of microbeads in the trapping chamber with 2.0 A current supply in the continuous microfluidics flow > 100 mL/min has also been demonstrated. In conclusion, a separation of biological cells labelled with magnetic microbeads is expected to be realized using the fabricated PDMS microchannel.
References
Plouffe, B.D., Murthy, S.K. and Lewis, L.H. 2014. Fundamentals and Application of Magnetic Particles in Cell Isolation and Enrichment: A Review. Rep. Prog. Phys. 78(1): 016601.
van Reenen, A., de Jong, A.M., den Toonder,J.M.J. and Prins, M.W.J. 2014. Integrated Lab-on-chip Biosensing Systems Based on Magnetic Particle Actuation--A Comprehensive Review. Lab Chip. 14(12): 1966–86.
Whitesides, G.M. 2006. The Origins and the Future of Microfluidics. Nature. 442(7101): 368–73.
Sia, S.K. and Whitesides, G.M. 2003. Microfluidic Devices Fabricated in Poly(dimethylsiloxane) for Biological Studies. Electrophoresis. 24(21): 3563–76.
Borlido, L., Azevedo,A.M., Roque,A.C.A. and Aires-Barros,M.R. 2013. Magnetic Separations in Biotechnology. Biotechnol. Adv. 31(8): 1374–85.
Pamme, N. 2006. Magnetism and Microfluidics. Lab Chip. 6(1): 24–38.
Abidin, U., Majlis, B.Y. and Yunas,J. 2015. Integrated High Magnetic Gradient System for Trapping Nanoparticles. J. Teknol. 75(11): 151-160.
Abidin, A., Majlis, B.Y. and Yunas, J. 2012. Design and Simulation of High Magnetic Gradient Device for Effective Bioparticles Trapping. 10th IEEE International Conference on Semiconductor Electronics (ICSE): 195–199.
Xia, Y. and Whitesides, G.M. 1998. Soft Lithography. Angew. Chemie Int. Ed. 37(5): 550–575.
Shaw, J.M., Gelorme, J.D., LaBianca, N.C., Conley, W.E. and Holmes, S.J. 1997. Negative Photoresists for Optical Lithography IBM J. Res. Dev. 41(1.2): 81–94.
Lee, K.Y. 1995. Micromachining Applications of a High Resolution Ultrathick Photoresist. J. Vac. Sci. Technol. B Microelectron. Nanom. Struct. 13(6): 3012.
Lorenz, H., Despont, M., Fahrni, N. , LaBianca,N., Renaud, P. and Vettiger, P. 1997. SU-8: A Low-cost Negative Resist for MEMS. J. Micromechanics Microengineering. 7(3): 121–124.
Tufts University. 2007. SU-8 Photoresist Processing.
MicroChem. 2000. SU-8 2000 Permanent Epoxy Negative Photoresist Processing Guidelines.
Spherotech. Magnetic Microparticles and Nanoparticles-Spherotech. http://www.spherotech. com/para_par.htm.
Haubert, K., Drier,T., and Beebe, D. 2006. PDMS Bonding by Means of a Portable, Low-cost Corona System. Lab Chip. 6(12): 1548–9.
Eddings, M.a., Johnson, M.a., and Gale,B.K. 2008. Determining the Optimal PDMS–PDMS Bonding Technique for Microfluidic Devices. J. Micromechanics Microengineering. 18(6): 067001.
Downloads
Published
Issue
Section
License
Copyright of articles that appear in Jurnal Teknologi belongs exclusively to Penerbit Universiti Teknologi Malaysia (Penerbit UTM Press). This copyright covers the rights to reproduce the article, including reprints, electronic reproductions, or any other reproductions of similar nature.
