INTRUSION OF POLYETHYLENE GLYCOL DURING OSMOTIC TESTS: IDENTIFYING CELLULOSE ACETATE DEGRADING MICROBES

Authors

  • Mohd Yuhyi Mohd Tadza Faculty of Civil Engineering & Earth Resources, Universiti Malaysia Pahang, Malaysia
  • Mohd Fazli Farida Asras Faculty of Industrial Science & Technology, Universiti Malaysia Pahang, Malaysia
  • Muhammad Afiq Mohd Tadza Faculty of Industrial Science & Technology, Universiti Malaysia Pahang, Malaysia
  • Jamil Ismail Faculty of Industrial Science & Technology, Universiti Malaysia Pahang, Malaysia
  • Nurhidayah Mahazam Faculty of Civil Engineering & Earth Resources, Universiti Malaysia Pahang, Malaysia
  • Nurul Syafiqah Mohd Azmi Faculty of Civil Engineering & Earth Resources, Universiti Malaysia Pahang, Malaysia

DOI:

https://doi.org/10.11113/jt.v78.9604

Keywords:

Clay, microbes, suction, osmotic, cellulose, polyethylene glycol

Abstract

Cellulose acetate semipermeable membranes and polyethylene glycol (PEG) solutions are commonly used to apply suction in soils using the osmotic technique. The structural integrity of the membrane is crucial to maintain a consistent suction value throughout a test. The membrane however, is vulnerable to microbial attack, which in turn could lead to intrusion of PEG into soil specimens. In this study, osmotic test was carried out on initially saturated Andrassy bentonite specimen. PEG 6000 and membrane with molecular weight cut-off (MWCO) value of 3500 was used to apply suction of 3.4 MPa. Soil specimen and PEG solution after the osmotic test were examined for the presence of any potential cellulose or acetate degrading microbes. Test results indicated that cellulose degrading fungi such as Paecilomyces L. and Trichoderma A. were observed in the PEG solutions. Addition of penicillin was found to be less effective in removing these microbes. However, 70% ethanol may be used to prevent cross contamination during handling of specimens. It is anticipated that eliminating these microbes is crucial to prevent intrusion of PEG in osmotic tests

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Published

2016-08-25

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Section

Science and Engineering

How to Cite

INTRUSION OF POLYETHYLENE GLYCOL DURING OSMOTIC TESTS: IDENTIFYING CELLULOSE ACETATE DEGRADING MICROBES. (2016). Jurnal Teknologi, 78(8-5). https://doi.org/10.11113/jt.v78.9604